Influence of freezing and non-freezing temperature on somatic embryogenesis and vinblastine production in Catharanthus roseus (L.) G.Don.

• Autorzy: Aslam J. , Mujib A. , Sharma M. P.
• Języki publikacji: EN

Abstrakty

EN

Catharanthus roseus (L.) G. Don. (Apocynaceae) is an important dicotyledonous medicinal plant. It produces vinblastine and vincristine, two alkaloids that are being used against a variety of cancers. In the present study, the freezing (-196, 4, 15°C) and non-freezing (25°C) temperature was imposed on embryogenic cultures, and later in vitro embryogeny and vinblastine production in C. roseus was studied. Somatic embryo (SE) production was maximum at 15°C, but the SE maturation was high at 4°C. The SEs, grown at 25°C, showed highest germination and plantlet conversion. Quantitative estimation of vinblastine was carried out using high-performance liquid chromatography in various in vitro raised tissues (embryogenic callus), embryo stages (proliferated, matured and germinated embryos)], and SE-derived plantlets (leaf, shoot, root and whole plant) after various freezing- and non-freezing temperature treatments. Vinblastine synthesis was temperature dependent in C. roseus that has been discussed in this present article.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 2
• Opis fizyczny: p.473-480,fig.,ref.

Twórcy

autor

Aslam J.

• Cellular Differentiation and Molecular Genetic Section, Department of Botany, Hamdard University, New Delhi 110062, India

autor

Mujib A.

• Cellular Differentiation and Molecular Genetic Section, Department of Botany, Hamdard University, New Delhi 110062, India

autor

Sharma M. P.

• Cellular Differentiation and Molecular Genetic Section, Department of Botany, Hamdard University, New Delhi 110062, India

Bibliografia

• Bewley JD, Black M (1994) Seeds: physiology of development and germination. Springer, Berlin
• Bhat MA, Ahmad S, Junaid A, Mujib A, Mahmooduzzafar (2008) Salinity stress enhanced production of solasodine in Solanum nigrum L. Chem Pharm Bull 56(1):17–21
• Carraway DT, Merkle SA (1997) Plantlet regeneration from somatic embryos of American chestnut. Can J For Res 27:1805–1812
• Cheong EJ, Pooler MR (2004) Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink. Plant Cell Rep 22:810–815
• Chu I, Bodnar JA, White EL, Bowman RN (1996) Quantification of vincristine and vinblastine in Catharanthus roseus plants by capillary zone electrophoresis. J Chromatogr 755:281–288
• Corredoira E, Ballester A, Vieitez AM (2003) Proliferation, maturation and germination of Castanea sativa Mill somatic embryos originated from leaf explants. Anal Bot 92:129–136
• Datta A, Srivastava PS (1997) Variation in vinblastine production by Catharanthus roseus during in vivo and in vitro differentiation. Phytochemistry 46:135–137
• Engelmann F (1997) In vitro conservation methods. In: Callow JA, Ford-Lloyd BV, Newbury HJ (eds) Biotechnology and plant genetic resources. CAB International, Oxford, pp 119–161
• Favretto D, Piovan A, Filippini R, Caniato R (2001) Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow-injection electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 15(5):364–369
• Hagmann HM, Ryynanen LA, Aronen TS, Krajinakova J (1998) Cryopreservation of embryogenic cultures of Scots pine. Plant Cell Tissue Organ Cult 54:45–53
• Hincha DK, Zuther E, Hundertmark M, Heyer AG (2006) The role of compatible solutes in plant freezing tolerance: a case study on Raffinose. In: Chen THH, Uemura M, Fujikawa S (eds) Cold hardiness in plants: molecular genetics, cell biology and physiology. CAB International, Oxford, pp 203–217
• Holliday C, Merkle SA (2000) Preservation of American chestnut germplasm by cryostorage of embryogenic cultures. J Am Chestnut Found 14:46–52
• Joy IV, Yeung RWEC, Kong L, Thorpe TA (1991) Development of white spruce somatic embryos: I. Storage product deposition. In Vitro Cell Dev Biol Plant 27:32–41
• Junaid A, Bhatt MA, Mujib A, Sharma MP (2006) Somatic embryo proliferation, maturation and germination in Catharanthus roseus. Plant Cell Tissue Organ Cult 84:325–332
• Junaid A, Mujib A, Sharma MP, Samaj J (2007a) Somatic embryogenesis and plant regeneration in Catharanthus roseus. Biol Plant 51(4):641–646
• Junaid A, Mujib A, Sharma MP, Tang W (2007b) Growth regulators affect primary and secondary somatic embryogenesis in Madagaskar periwinkle (Catharanthus roseus (L.) G Don.) at morphological and biochemical levels. Plant Growth Regul 51:271–281
• Junaid A, Mujib A, Fatima S, Sharma MP (2008) Culture condition affect somatic embryogenesis in Catharanthus roseus L (G.) Don. Plant Biotechnol Rep 2:179–190
• Junaid A, Mujib A, Nasim SA, Sharma MP (2009) Screening of vincristine yield in ex vitro and in vitro somatic embryos derived plantlets of Catharanthus roseus L. (G) Don. Sci Hortic 119:325–329
• Karam NS, Al-Majathoub M (2000) Direct shoot regeneration and microtuberization in wild Cyclamen persicum Mill using seedling tissue. Sci Hortic 86:235–246
• Kuboyama T, Yokoshima S, Tokuyama H, Fukuyama T (2004) Stereocontrolled total synthesis of (+) vincristine. PNAS 101:11966–11970
• Lizbeth A, Castro-Concha L, Maria Escobedo R, de Lourdes Miranda-Ham M (2005) Measurement of cell viability protocol. Plant Cell Cult Protocol 318:71–76
• Mathur G, Alkutkar VA, Nadgauda RS (2003) Cryopreservation of embryogenic culture of Pinus roxburghii. Biol Plant 46:205–210
• Meijer AH, Verpoorte R, Hoge JHC (1993) Regulation of enzymes and genes involved in terpenoids indole alkaloid biosynthesis in Catharanthus roseus. J Plant Res 3:145–164
• Merkle SA, Parrot WA, Flinn BS (1995) Morphogenetic aspects of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer, Dordrecht, pp 155–203
• Miura Y, Hirata K, Kurano KN, Miyamoto K, Chiida K (1988) Formation of vinblastine in multiple shoot culture of Catharanthus roseus. Planta Med 54:18–20
• Moreno PRH, Van der Heijden R, Verpoorte R (1995) Cell, tissue cultures of Catharanthus roseus; a literature survey II. Updating from 1988–1993. Plant Cell Tissue Organ Cult 42:1–25
• Moriguchi T, Kozaki I, Yamaki S, Sanada T (1990) Low temperature storage of Pear shoots in vitro. Bull Fruit Tree Res Stn 17:11–18
• Mujib A, Samaj J (2006) Somatic embryogenesis. Springer, Berlin Mukherjee AK, Basu S, Sarkar N, Ghosh AC (2001) Advances in cancer therapy with plant based natural products. Curr Med Chem 8:1467–1486
• Pan ZG, Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2003) Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra. Plant Sci 165:681–687
• Pande D, Purohit M, Srivastava PS (2002) Variation in xanthotoxin content in Ammi majus L. cultures during in vitro flowering and fruiting. Plant Sci 162:583–587
• Pasquali G, Goddijin OJM, De Wall A, Verpoorte R, Schilperoort RA, Hoge JHC (1992) Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors. Plant Mol Biol 18:1121–1131
• Pullman GS, Gupta PK, Timmis R, Carpenter C, Kreitinger M, Welty E (2005) Improved Norway spruce somatic embryo development through the use of abscisic acid combined with activated carbon. Plant Cell Rep 24:271–279
• Ramage CM, Williams RR (2004) Cytokinin induced abnormal shoot regeneration is associated with elevated knottedl—type homeobox gene expression in tobacco. Plant Cell Rep 22:919–924
• San-José MC, Ballester A, Vieitez AM (2001) Effect of thidiazuron on multiple shoot induction and plant regeneration from cotyledonary nodes of chestnut. J Hortic Sci Biotechnol 76:588–595
• Stasolla C, Loukanina N, Ashihara H, Yeung EC, Thorpe TA (2002) Pyrimidine nucleotide biosynthesis and nucleic acid metabolism in embryos and megagametophytes of white spruce (Picea glauca) during germination. Physiol Plant 115:155–165
• Thorpe TA (1995) In vitro embryogenesis in plants. Kluwer, Dordrecht
• Van der Heijden R, Verpoorte R, Ten Hoopen HJG (1989) Cell and tissue cultures of Catharanthus roseus (L) G. Don: a literature survey. Plant Cell Tissue Organ Cult 18:231–280
• Van der Heijden R, Jacobs DT, Snoeijer W, Hallard D, Verpoorte R (2004) The Catharanthus alkaloids: pharmacognosy and biochemistry. Curr Med Chem 11:607–628
• Verpoorte R, Van der Heijden R, Schripsema J, Hoge JHC, Ten Hoopen HJG (1993) Plant biotechnology for the production of alkaloids: present status and prospects. J Nat Prod 56:186–207
• Winkelmann T, Mubmann V, Serek M (2004) Cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Mill. Plant Cell Rep 23:1–8
• Xiao L, Koster KL (2001) Desiccation tolerance of protoplasts isolated from pea embryos. J Exp Bot 52:2105–2114
• Xin Z, Browse J (2000) Cold comfort farm: the acclimation of plants to freezing temperatures. Plant Cell Environ 23:893–902
• Xing Z, Powell WA, Maynard CA (1999) Development and germination of American chestnut somatic embryos. Plant Cell Tissus Organ Cult 57:47–55

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