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2009 | 53 | 4 |

Tytuł artykułu

Antibiotic resistance and Beta-lactamases of Yersinia enterocolitica isolated from pigs in Poland

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Sixty-two isolates of pig-derived Yersinia enterocolitica belonging to 1A and 4 biotypes and non-typeable isolates were subjected to antimicrobial resistance testing by the disc diffusion method. The presence of β-lactamase A (Bla-A) and B (Bla-B) was detected by the double disc diffusion test, and the β-lactamase genes blaA and blaB in these isolates were detected by PCR. The isolates were resistant or moderately susceptible mostly to penicillins (except piperacillin) and first-generation cephalosporins. The majority of the isolates of biotype 1A were resistant or moderately susceptible to amoxicillin/clavulanic acid. Three isolates of biotype 4 were also resistant to amoxicillin/clavulanic acid. Most of Y. enterocolitica isolates were resistant or intermediately susceptible to second-generation cephalosporins. Cefotaxime was the most active third-generation cefalosporin against the tested Y. enterocolitica. No isolate was resistant to imipenem. Tetracyclines, aminoglycosides, and quinolones showed high activity against Y. enterocolitica. The presence of the Bla-A enzyme was found in 27/31 isolates of biotype 1A, 22/28 isolates of biotype 4, and in all untypeable isolates. In these isolates the blaA gene was detected by PCR amplification. In all Y. enterocolitica isolates blaB gene was detected but not all isolates of biotype 4 showed the presence of Bla-B.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

53

Numer

4

Opis fizyczny

p.603-607,fig.,ref.

Twórcy

autor
  • Department of Microbiology, Institute of Biology, University of Podlasie, 08-110 Siedlce, Poland
autor
  • Department of Microbiology, Institute of Biology, University of Podlasie, 08-110 Siedlce, Poland

Bibliografia

  • 1. Abdel-Haq N.M., Papadopol R., Asmar B.I., Brown W.J.: Antibiotic susceptibilities of Yersinia enterocolitica recovered from children over a 12-year period. Int J Antimicrob Agents 2006, 27, 449-452.
  • 2. Baumgartner A., Kűffer M., Suter D., Jemmi T., Rohner P.: Antimicrobial resistance of Yersinia enterocolitica strains from human patients, pigs and retail pork in Switzerland. Int J Food Microbiol 2007, 115, 110-114.
  • 3. Bottone E.J.: Yersinia enterocolitica: overview and epidemiologic correlates. Microbes Infect 1999, 1, 323- 333.
  • 4. Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial disk susceptibility tests; approved standard. Wayne, USA, 2006.
  • 5. EFSA (European Food Safety Agency): Foodborne antimicrobial resistance as a biological hazard. EFSA J 2008, 765.
  • 6. EFSA (European Food Safety Agency): The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2005. EFSA J 2006, 94.
  • 7. Kechagia N., Nicolaou C., Ioannidou V., Kourti E., Ioannidis A., Legakis N.J., Chatzipanagiotou S.: Detection of chromosomal and plasmid - encoded virulence determinants in Yersinia enterocolitica and other Yersinia spp. isolated from food animals in Greece. Int J Food Microbiol 2007, 118, 326-331.
  • 8. Kot B., Jakubczak A., Piechota M.: Isolation and characterization of Yersinia enterocolitica rods from pig tonsils. Medycyna Wet 2008, 64, 283-287.
  • 9. Pham J.N., Bell S.M., Martin L., Carniel E.: The β- lactamases and β-lactam antibiotic susceptibility of Yersinia enterocolitica. J Antimicrob Chemother 2000, 46, 951-957.
  • 10. Rastawicki W., Gierczyński R., Jagielski M., Kałużewski S., Jeljaszewicz J.: Susceptibility of Polish clinical strains of Yersinia enterocolitica serotype O3 to antibiotics. Int J Antimicrob Agents 2000, 13, 297-300.
  • 11. Sharma S., Mittal S., Mallik S., Virdi J.S.: Molecular characterization of β-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A. FEMS Microbiol Lett 2006, 257, 319-327.
  • 12. Sharma S., Ramnani P., Virdi J.S.: Detection and assay of β-lactamases in clinical and non-clinical strains of Yersinia enterocolitica biovar 1A. J Antimicrob Chemother 2004, 54, 401-405.
  • 13. Simonova J., Borilova G., Steinhauserova I.: Occurrence of pathogenic strains of Yersinia enterocolitica in pigs and their antimicrobial resistance. Bull Vet Inst Pulawy 2008, 52, 39-43.
  • 14. Stock I., Heisig P., Wiedemann B.: Expression of β- lactamases in Yersinia enterocolitica strains of biovars 2, 4 and 5. J Med Microbiol 1999, 48, 1023-1027.
  • 15. Stock I., Heisig P., Wiedemann B.: β-lactamase expression in Yersinia enterocolitica biovars 1A, 1B and 3. J Med Microbiol 2000, 49, 403-408.
  • 16. White D.G., Zhao S., Simjee S., Wagner D.D., McDermott P.F.: Antimicrobial resistance of foodborne pathogenes. Microbes Infect 2002, 4, 405-412.
  • 17. Zheng H., Sun Y., Lin S., Mao Z., Jiang B.: Yersinia enterocolitica infection in diarrheal patients. Eur J Clin Microbiol Infect Dis 2008, 27, 741-752.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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