Osthole improves collagen-induced arthritis in a rat model through inhibiting inflammation and cellular stress

• Autorzy: Xu R. , Liu Z. , Hou J. , Huang T. , Yang M.
• Języki publikacji: EN

Abstrakty

EN

Background: Osthole is a natural product that has multiple bioactive functions and has been reported to exert potent immunosuppressive effects. However, the therapeutic effect of osthole on arthritis has not been explored. In the present study, a collagen-induced arthritis rat model, IL-1β-stimulated SW982 cells, and RA-like fibroblast-like synoviocytes (FLS) were employed to investigate the effect and possible mechanism of osthole on arthritis in vivo and in vitro. Results: 20 and 40 mg/kg osthole significantly alleviated collagen-induced arthritic symptoms based on histopathology and clinical arthritis scores, and improved erosion using HE staining. 20 and 40 mg/kg osthole decreased the level of IL-1β, TNF-α and IL-6 in rats and ameliorated oxidative stress in serum evaluated using ELISA kits. In addition, treatment with 50 and 100 μM osthole for 48 h inhibited 10 ng/ml IL-1β-stimulated proliferation and migration of SW982, and significantly inhibited the expression of matrix metalloproteinases, such as MMP-1, MMP-3 and MMP-13, as detected by western blot. 50 and 100 μM osthole also blocked the generation of IL-6 and TNF-α in IL-1β-stimulated SW982 cells. The NF-κB and MAPK pathways were also inhibited by osthole in IL-1β-treated SW982 cells. Conclusion: These results collectively demonstrated that osthole improves collagen-induced arthritis in a rat model and IL-1β-treated SW982 cells through inhibiting inflammation and cellular stress in vivo and in vitro, and osthole might be a promising therapeutic agent for RA.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-11,fig.,ref.

Twórcy

autor

Xu R.

• Department of Osteology, YeDa Hospital, Taishan Road No. 11 Economic and Technological District of Yantai, Yantai 264006, China

autor

Liu Z.

• Department of Osteology, YeDa Hospital, Taishan Road No. 11 Economic and Technological District of Yantai, Yantai 264006, China

autor

Hou J.

• Department of Osteology, YeDa Hospital, Taishan Road No. 11 Economic and Technological District of Yantai, Yantai 264006, China

autor

Huang T.

• Department of Osteology, YeDa Hospital, Taishan Road No. 11 Economic and Technological District of Yantai, Yantai 264006, China

autor

Yang M.

• Department of Osteology, YeDa Hospital, Taishan Road No. 11 Economic and Technological District of Yantai, Yantai 264006, China

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Identyfikatory

DOI

10.1186/s11658-018-0086-0