Cryptosporidiosis outbreak on a dairy farm: Detection of Cryptosporidium parvum as a causative agent in the water source

• Autorzy: Karakavuk M. , Can H. , Doskaya M. , Karakavuk T. , Erkunt-Alak S. , Koseoglu A.E. , Gul A. , Un C. , Guruz Y. , Degirmenci-Doskaya A.
• Języki publikacji: EN

Abstrakty

EN

Diarrhea caused by parasitic agents is common in neonatal calves and leads to significant economic losses in cattle farms worldwide. Cryptosporidium spp. is one of the most frequently detected parasitic agents causing diarrhea in neonatal calves. The aim of this study was to investigate the presence of Cryptosporidium spp. on a dairy farm which a has major diarrhea problem. Samples were collected from calves, cows, drinking bowls, and two different artesian water sources, as well as from the environment. All fecal samples were investigated using Kinyoun acid-fast stained slides and real-time PCR targeting the Cryptosporidium spp. COWP gene. In addition, species identification was performed by nested PCR targeting the Cryptosporidium spp. COWP gene and sequencing. Cryptosporidium spp. was detected in 11 calves (30.55%; 11/36) by real-time PCR and the cows were negative. Among real-time PCR positive samples, only five were also found positive by microscopy. Moreover, Cryptosporidium spp. was found in one of the two artesian water sources and five environmental samples by real-time PCR. Among these positive samples, eight were sequenced. According to the RFLP pattern, BLAST and, phylogenetic analyses, all sequenced samples were Cryptosporidium parvum. These findings show the importance of C. parvum as a cause of calf diarrhea on dairy farms.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2021
• Tom: 24
• Numer: 3
• Opis fizyczny: p.323-333,fig.,ref.

Twórcy

autor

Karakavuk M.

• Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey
• Veterinary technology programs, Ege University, Ödemiş Vocational School, Ödemiş, Izmir, Turkey

autor

Can H.

• Department of Biology, Molecular Biology Section, Ege University Faculty of Science, Bornova, İzmir, Turkey

autor

Doskaya M.

• Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey

autor

Karakavuk T.

• Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey

autor

Erkunt-Alak S.

• Department of Biology, Molecular Biology Section, Ege University Faculty of Science, Bornova, İzmir, Turkey,

autor

Koseoglu A.E.

• Department of Biology, Molecular Biology Section, Ege University Faculty of Science, Bornova, İzmir, Turkey

autor

Gul A.

• Department of Bioengineering, Ege University Faculty of Engineering, Bornova, İzmir, Turkey

autor

Un C.

• Department of Biology, Molecular Biology Section, Ege University Faculty of Science, Bornova, İzmir, Turkey

autor

Guruz Y.

• Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey

autor

Degirmenci-Doskaya A.

• Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey

Identyfikatory

DOI

10.24425/pjvs.2021.137669