Genetic variability of Pinus sylvestris populations from IUFRO 1982 provenance trial

• Autorzy: Androsiuk P. , Urbaniak L.
• Języki publikacji: EN

Abstrakty

EN

Provenance trials were designed to analyse the quantitative responses of tree species to environmental variables found in different experiment location. However, we have still limited knowledge how natural and artificial selection affects genetic variation of the species populations gather in such experimental sites. We have used bulked DNA-based RAPD and ISJ analysis to investigate genetic diversity and differentiation of Scots pine populations from two Polish locations of IUFRO 1982 provenance trial placed in Kórnik and in Supraśl. Applied categories of DNA markers differed in terms of revealing genetic diversity of the species. Ten RAPD primers applied in the study yielded a total of 75 bands, of which 21 (28%) and 15 (20%) were polymorphic in Kórnik and in Supraśl, respectively. Six ISJ primers revealed 42 bands of which 4 (9.52%) and 14 (33.3%) were polymorphic in Kórnik and in Supraśl, respectively. The genetic diversity and differentiation was low, as expressed by He=0.071 and He=0.085, and by genetic distance values which ranged from 0.0 to 0.240 (on average 0.081) and from 0.017 to 0.188 (on average 0.094) for Kórnik and Supraśl, respectively. Location of provenance trial appeared to have a significant influence on revealed level of genetic polymorphism and pattern of interpopulation differentiation. However, genetic structure found for analysed Scots pine provenances from IUFRO 1982 in Kórnik was also confirmed for Supraśl experimental site. In the light of available data we also discussed the influence of historical migration routes and gene flow on observed genetic variation of the species.

Słowa kluczowe

EN

genetic variability; Scotch pine; Pinus sylvestris; plant population; molecular marker; provenance trial

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2014
• Tom: 71
• Opis fizyczny: p.23-33,fig.,ref.

Twórcy

autor

Androsiuk P.

• Department of Plant Physiology, Genetics, and Biotechnology, University of Warmia and Mazury in Olsztyn, Pl.Lodzki 3, 10-719 Olsztyn, Poland

autor

Urbaniak L.

• Department of Genetics, Adam Mickiewicz University, Poznan, Poland

Bibliografia

• Androsiuk P., Kaczmarek Z., Urbaniak L. 2011a. The morphological traits of needles as markers of geographical differentiation in European Pinus sylvestris populations. Dendrobiology 65: 3–16.
• Androsiuk P., Zieliński R., Polok K. 2011b. B-SAP markers derived from the bacterial KatG gene differentiate populations of Pinus sylvestris and provide new insights into their postglacial history. Silva Fennica 45: 3–18.
• Barzdajn W. 2000. A provenance experiment on variability of Scots pine (Pinus sylvestris L.) in the IUFRO 1982 series in the Supraśl Forest District. Sylwan 146: 41–52. (in Polish with English summary)
• Barzdajn 2008. Results of a 24 years provenance trial in the Supraśl Forest District. Sylwan 152: 21–29. (in Polish with English summary)
• Bennett K.D., Tzedakis P.C., Willis K.J. 1991. Quaternary refugia of north European trees. Journal of Biogeography 18: 103–115.
• Birks H.J.B., Line J.M. 1993. Glacial refugia of European trees a matter of chance? Dissertationes Botanicae 196: 283–291.
• Boratyński A. 1993. Systematics and geographical distribution. In: Biology of Scots pine. Białobok S., Boratyński A., Bugała W. (eds.). Instytut Dendrologii PAN – Sorus, Poznań. pp.: 45–70.
• Cheddadi R., Vendramin G.G., Litt T., François L., Kageyama M., Lorentz S., Laurent J.M., Beaulieu J.L, Sadori L., Jost A., Lunt D. 2006. Imprints of glacial refugia in the modern genetic diversity of Pinus sylvestris. Global Ecology and Biogeography 15: 271–282.
• Excoffier L., Smouse P., Quattro J.M. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479–491.
• Excoffier L., Laval G., Schneider S. 2005. Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1: 47–50.
• García-Gil M.R., Mikkonen M., Savolainen O. 2003. Nucleotide diversity at two phytochrome loci along a latitudinal cline in Pinus sylvestris. Molecular Ecology 12: 1195–1206.
• Giertych M. 1979. Summary of results on Scots pine (Pinus sylvestris L.) height growth in IUFRO provenance experiments. Silvae Genetica 28: 136–152.
• Giertych M., Mátyás, Cs. (ed.). 1991. Genetics of Scots Pine. Budapest: Akadémiai Kiadó. pp. 280.
• Giertych M., Oleksyn J. 1992. Studies on Genetic Variation in Scots pine (Pinus sylvestris L.) coordinated by IUFRO. Silvae Genetica 41: 133–143.
• Goto S, Miyahara F., Ide Y. 2001. A fast method for checking the genetic identity of ramets in a clonal seed orchard by RAPD analysis with a bulking procedure. Silvae Genetica 50: 271–275.
• Herrmann D., Boller B., Widmer F., Kolliker R. 2005. Optimization of bulked AFLP analysis and its application for exploring diversity of natural and cultivated populations of red clover. Genome 48: 474–486.
• Huntley B., Birks H.J.B. 1983. An atlas of past and present pollen maps for Europe: 0–13000 years ago. Cambridge University press, Cambridge.
• Kożuchowski K., Degirmendžić J. 2005. Contemporary changes of climate in Poland: trends and variation in thermal and solar conditions related to plant vegetation. Polish Journal of Ecology 53: 283–297.
• Luoma S. 1997. Geographical pattern in photosynthetic light response of Pinus sylvestris in Europe. Functional Ecology 11: 273–281.
• Meusel H., Jäger E., Weinert E. 1965. Vergleichende Chorologie der Zentraleuropäischen Flora: Text/Hrsg. von Hermann Meusel gemainsam mit E.Jäger und E. Weinert. Jena: Veb Gustav Fisher Verlag, pp. 583.
• Murray M.G., Thompson W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research 8: 4321–4325.
• Myczko Ł. 2005. Changes in genetic structure of groups of Pinus sylvestris L. seedlings as a result of silvicultural actions. Doctoral dissertation, Adam Mickiewicz University, Poznań. (in Polish)
• Naydenov K., Senneville S., Beaulieu J., Tremblay F., Bousquet J. 2007. Glacial vicariance in Eurasia: mitochondrial DNA evidence from Scots pine for a complex heritage involving genetically distinct refugia at mid-northern latitudes and in Asia Minor. BMC Evolutionary Biology 7: 233.
• Nei M. 1972. Genetic distance between populations. American Naturalist 106: 283–292.
• Newton A.C., Allnutt T.R., Gilles A.C.M., Lowe A.J., Ennos R.A. 1999. Molecular phyleography, intraspecific variation and the conservation of tree species. Trends in Ecology and Evolution 14: 140–141.
• Newton A.C., Allnutt T.R., Dvorak W.S., Del Castillo R.F., Ennos R.A. 2002. Patterns of genetic variation in Pinus chiapensis, a threatened Mexican pine, detected by RAPD and mitochondrial DNA RFLP markers. Heredity 89: 191–198.
• Oleksyn J. 1988. Report on the IUFRO 1982 provenance experiment on Scots pine (Pinus sylvestris L.). Arboretum Kórnickie 33: 211–229.
• Oleksyn J. 1993. Differentiated susceptibility to harmful abiotic factors. In: Biology of Scots pine. Białobok S., Boratyński A., Bugała W. (eds.). Sorus Press, Poznań–Kórnik, pp. 395–404. (in Polish)
• Oleksyn J., Tjoelker M.G., Reich P.B. 1992a. Growth and biomass partitioning of populations of European Pinus sylvestris L. under simulated 50° and 60° N daylengths: evidence for photoperiodic ecotypes. New Phytologist 120: 561–574.
• Oleksyn J., Tjoelker M.G., Reich P.B. 1992b. Whole-plant CO2 exchange of seedlings of two Pinus sylvestris L. Provenances grown under simulated photoperiodic conditions of 500 and 600 N. Trees 6: 225–231.
• Oleksyn J., Prus-Głowacki W., Giertych M., Reich P.B. 1994. Relation between genetic diversity and pollution impact in a 1912 experiment with East European Pinus sylvestris provenances. Canadian Journal of Forest Research 24: 2390–2394.
• Oleksyn J., Reich P.B., Chalupka W., Tjoelker M.G. 1999. Differential above- and belowground biomass accumulation of European Pinus sylvestris populations in a 12-year-old provenance experiment. Scandinavian Journal of Forest Research 14: 7–17.
• Oleksyn J., Reich P.B., Rachwał L., Tjoelker M.G., Karolewski P. 2000. Variation in aboveground net primary production of diverse European Pinus sylvestris populations. Trees 14: 415–421.
• Oleksyn J., Reich P.B., Tjoelker M.G., Chalupka W. 2001. Biogeographic differences in shoot elongation pattern among European Scots pine populations. Forest Ecology and Management 148: 207–220.
• Oleksyn J., Reich P.B., Zytkowiak R., Karolewski P., Tjoelker M.G. 2003. Nutrient conservation increases with latitude of origin in European Pinus sylvestris populations. Oecologia 136: 220–135.
• Polok K., Urbaniak L., Korzekwa K., Androsiuk P., Ciągło S., Kubiak K., Zieliński R. 2005. Genetic similarity of Pinus sylvestris populations on the base of DNA markers. In: Prus-Głowacki W., Pawlaczyk E. (eds.). Variability and Evolution – New Perspectives: 253–267. Wyd. UAM. Poznań.
• Polok K. 2007. Molecular evolution of the genus Lolium L. SQL, Olsztyn. 318 pp.
• Pravdin L.F. 1964. Scots pine variation, intraspecific taxonomy and selection. Academia Nauk SSSR. 208 p. [English translation TT69-55066. Springfield, VA: USDC CFSTI].
• Prus-Głowacki W., Stephan B.R. 1994. Genetic variation of Pinus sylvestris from Spain in relation to other European populations. Silvae Genetica 43: 7–14.
• Prus-Głowacki W., Urbaniak L., Żubrowska-Gil M. 1993. Allozyme differentiation in some European populations of Scots pine (Pinus sylvestris L.). Genetica Polonica 34: 159–176.
• Reich P.B., Oleksyn J., Tjoelker M.G. 1994. Seed mass effects on germination and growth of diverse European Scots pine populations. Canadian Journal of Forest Research 24: 306–320.
• Reif J.C., Hamrit S., Heckenberger M., Schipprack W., Maurer H.P., Bohn M., Melchinger A.E. 2005. Genetic structure and diversity of European flint maize populations determined with SSR analyses of individuals and bulks. Theoretical and Applied Genetics 111: 906–913.
• Rzeźnik Z. 1991. Scots pine (Pinus sylvestris L.) of European provenances in the Supraśl forest inspectorate. Roczniki Akademii Rolniczej w Poznaniu Rozprawy Naukowe 219: 55–67. (in Polish with English summary)
• Sinclair W.T., Morman J.D., Ennos R.A.1999. The postglacial history of Scots pine (Pinus sylvestris L.) in western Europe: evidence from mitochondrial DNA variation. Molecular Ecology 8: 83–88.
• Soranzo N., Alia R., Provan J., Powell W. 2000. Patterns of variation at a mitochondrial sequence-tagged-site locus provides new insights into the postglacial history of European Pinus sylvestris populations. Molecular Ecology 9: 1205–1211.
• Stephan B.R., Liesbach M. 1996. Results of the IUFRO 1982 Scots pine (Pinus sylvestris L.) provenance experiment in southwestern Germany. Silvae Genetica 45: 342–349.
• Szczecińska M., Sawicki J., Polok K., Hołdyński Cz., Zieliński R. 2006. Comparison of the Polygonatum species from Poland based on DNA markers. Annales Botanici Fennici 43: 379–388
• Szczecińska M., Sawicki J., Wąsowicz K, Hołdyński Cz. 2009. Genetic variation of the relict and endangered population of Chamaedaphne calyculata (Ericaceae) in Poland. Dendrobiology 62: 23–33.
• Szmidt A.E., Wang X.-R., Lu M.-Z. 1996. Empirical assessment of allozyme and RAPD variation in Pinus sylvestris L. using haploid tissue analysis. Heredity 76: 412–420.
• Urbaniak L. 1997. Biometric characters of seeds and wings as markers of geographical differentiation between European Scots pine (Pinus sylvestris L.) provenances. Acta Societatis Botanicorum Poloniae 66: 371–378.
• Urbaniak L. 1998. Differentiation of Scots pine (Pinus sylvestris L.) from the area of Eurasia on the basis of anatomical and morphological characters of needles. Adam Mickiewicz University Press, Poznań. (in Polish with English summary)
• Weining S., Langridge P. 1991. Identification and mapping of polymorphisms in cereals based on polymerase chain reaction. Theoretical and Applied Genetics 82: 209–216.
• Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
• Wójnicka-Półtorak A. 1997. Microevolutionary processes in Pinus sylvestris L. populations under industrial polutions. Doctoral dissertation, Adam Mickiewicz University, Poznań. (in Polish)
• Xia T., Meng L., Mao K., Tian B., Miehe G., Liu J. 2008. Genetic variation in the Qinghai-Tibetan Plateau endemic and endangered conifer Cupressus gigantea, detected using RAPD and ISSR markers. Silvae Genetica 57: 85–92.
• Yeh F.C., Yang R.C., Boyle T.B.J., Ye Z.H., Mao J.X. 2000. POPGENE 32, Microsoft Windows based Software for Population Genetic Analysis. Version 1.32, Molecular Biology and Biotechnology Centre, University of Alberta, Alberta, Canada

Identyfikatory

DOI

10.12657/denbio.071.003