A microsatellite study in the Łęgucki Młyn/Popielno hybrid zone reveals no genetic differentiation between two chromosome races of the common shrew (Sorex araneus)

• Autorzy: Moska M. , Wierzbicki H. , Macierzynska A. , Strzala T. , Maslak R. , Warchalowski M.
• Języki publikacji: EN

Abstrakty

EN

This study investigated a chromosome hybrid zone between two chromosomal races of the common shrew (Sorex araneus). Gene flow and genetic structure of the hybrid zone, located in the northeast of Poland, were studied using seven polymorphic autosomal microsatellite loci (L9, L14, L33, L45, L67, L68, L97) and a Y-linked microsatellite locus (L8Y). Seventy-five animals (46 of the Łęgucki Młyn race and 29 of the Popielno race) from nine different localities were examined and the data were analyzed using hierarchical AMOVA and F-statistic. The studied microsatellite loci and races (divided into nine geographical populations) were characterized by observed heterozygosity (H O), expected heterozygosities within (H S), and between (H T) populations, inbreeding coefficient (F IS), fixation index (F ST), and average allelic richness (A). We found that genetic structuring within and between the two chromosome races were weak and non-significant. This finding and unconstrained gene flow between the races indicates a high level of migration within the Łęgucki Młyn/Popielno hybrid zone, suggesting that evolutionarily important genetic structuring does not occur in interracial zones where races which are not genetically distinct come into contact.

• Wydawca: -
• Czasopismo: Acta Theriologica
• Rocznik: 2011
• Tom: 56
• Numer: 2
• Opis fizyczny: p.117-122,fig.,ref.

Twórcy

autor

Moska M.

• Department of Genetics and Animal Breeding, Wroclaw University of Environmental and Life Sciences, Kozuchowska 7, 51-631 Wroclaw, Poland

autor

Wierzbicki H.

• Department of Genetics and Animal Breeding, Wroclaw University of Environmental and Life Sciences, Kozuchowska 7, 51-631 Wroclaw, Poland

autor

Macierzynska A.

• Department of Genetics and Animal Breeding, Wroclaw University of Environmental and Life Sciences, Kozuchowska 7, 51-631 Wroclaw, Poland

autor

Strzala T.

• Department of Genetics and Animal Breeding, Wroclaw University of Environmental and Life Sciences, Kozuchowska 7, 51-631 Wroclaw, Poland

autor

Maslak R.

• Institute of Zoology, Wroclaw University, Sienkiewicza 21, 50-335 Wroclaw, Poland

autor

Warchalowski M.

• Department of Genetics and Animal Breeding, Wroclaw University of Environmental and Life Sciences, Kozuchowska 7, 51-631 Wroclaw, Poland

Bibliografia

• Andersson A-C (2004) Postglacial population history of the common shrew (Sorex araneus) in Fennoscandia. Ph.D. Thesis, Uppsala University
• Andersson A-C, Narain Y, Tegelström H, Fredga K (2004) No apparent reduction of gene flow in a hybrid zone between the West and North European karyotypic groups of the common shrew, Sorex araneus. Mol Ecol 13:1205–1215. doi:10.​1111/​j.​1365-294X.​2004.​02146.​x
• Balloux F, Ecoffey E, Fumagalli L, Goudet J, Wyttenbach A, Hausser J (1998) Microsatellite conservation, polymorphism, and GC content in shrews of the genus Sorex (Insectivora, Mammalia). Mol Biol Evol 15:473–475
• Balloux F, Lugon-Moulin N, Hausser J (2000) Estimating gene flow across hybrid zones: how reliable are microsatellites? Acta theriol 45(suppl 1):93–101
• Basset P, Yannic G, Yang F, O'Brien PCM, Graphodatsky AS, Ferguson-Smith MA, Balmus G, Volobouev VT, Hausser J (2006) Chromosome localization of microsatellite markers in the shrews of the Sorex araneus group. Chromosome Res 14:253–262. doi:10.​1007/​s10577-006-1041-x
• Brünner H, Lugon-Moulin N, Hausser J (2002a) Alps, genes, and chromosomes: their role in the formation of species in the Sorex araneus group (Mammalia, Insectivora), as inferred from two hybrid zones. Cytogenet Genome Res 96:85–96
• Brünner H, Lugon-Moulin N, Balloux F, Fumagalli L, Hausser J (2002b) A taxonomical re-evaluation of the Valais chromosome race of the common shrew Sorex araneus (Insectivora: Soricidae). Acta Theriol 47:245–275
• Excoffier L (2000) Analysis of population subdivision. In: Balding D, Bishop M, Cannings C (eds) Handbook of statistical genetics. Wiley and Sons, New York, NY. doi:10.​1002/​0470022620.​bbc25
• Excoffier L, Smouse P, Quattro J (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial restriction date. Genetics 131:479–491
• Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinfo Online 1:47–50
• Fedyk S, Wójcik JM, Chętnicki W, Mączewski S (2008) Invalidation of Stobnica chromosome race of the common shrew, Sorex araneus. Acta Theriol 53:375–380
• Fredga K (1996) The chromosome races of Sorex araneus in Scandinavia. Hereditas 125:123–135. doi:10.​1111/​j.​1601-5223.​1996.​00123.​x
• Fredga K, Narain Y (2000) The complex hybrid zone between Abisko and Sidensjö chromosome races of Sorex araneus in Sweden. Biol J Linn Soc 70:285–307. doi:10.​1006/​bijl.​1999.​0378
• Gaggiotti OE, Lange O, Rassmann K, Gliddon C (1999) A comparison of two indirect methods for estimating average levels of gene flow using microsatellites data. Mol Ecol 8:1513–1520. doi:10.​1046/​j.​1365-294x.​1999.​00730.​x
• Jadwiszczak KA (2002) Chromosome structure of the hybrid zone between the Drnholec and Białowieża races of the common shrew, Sorex araneus Linnaeus, 1758. Ph.D. Thesis, Białystok University. [In Polish]
• Jadwiszczak KA, Ratkiewicz M, Banaszek A (2006) Analysis of molecular differentiation in a hybrid zone between chromosomally distinct races of the common shrew Sorex araneus (Insectivora: Soricidae) suggests their common ancestry. Biol J Linn Soc 89:79–90. doi:10.​1111/​j.​1095-8312.​2006.​00659.​x
• Lugon-Moulin N, Balloux F, Hausser J (2000) Genetic differentiation of common shrew Sorex araneus populations among different alpine valleys revealed by microsatellites. Acta theriol 45(suppl 1):103–117
• Lugon-Moulin N, Hausser J (2002) Phylogenetical structure, postglacial recolonization and barriers to gene flow in the distinctive Valais chromosome race of the common shrew (Sorex araneus). Mol Ecol 11:785–794
• Moska M (2003) A hybrid zone between the chromosomal races Łęgucki Młyn and Popielno of the common shrew (Sorex araneus) in north-eastern Poland: preliminary results. Acta Theriol 48(4):441–455
• Narain Y, Fredga K (1996) A hybrid zone between Hällefors and Uppsala chromosome races of Sorex araneus in central Sweden. Hereditas 125:137–145. doi:10.​1111/​j.​1601-5223.​1996.​00137.​x
• User’s Guide SAS (2000) Version 8.0 Edition. SAS InstituteInc, Cary, NC, USA
• Searle JB, Wójcik JM (1998) Chromosomal evolution: the case of Sorex araneus. In: Wójcik JM, Wolsan M (eds) Evolution of Shrews. Mammal Research Institute, Polish Academy of Sciences, Białowieża, pp 219–268
• Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite date. Mol Ecol Notes 4:535–538. doi:10.​1111/​j.​1471-8286.​2004.​00684.​x
• Weir BC, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370
• White TA, Searle JB (2007) Genetic diversity and population size: island populations of the common shrew, Sorex araneus. Mol Ecol 16:2005–2016. doi:10.​1111/​j.​1365-294X.​2007.​03296.​x
• White TA, Bordewich M, Searle JB (2010) A network approach to study karyotypic evolution: the chromosomal races of the common shrew (Sorex araneus) and house mouse (Mus musculus) as model systems. Syst Biol 59(3):262–276. doi:10.​1093/​sysbio/​syq004
• Wójcik JM (1986) Karyotypic races of the common shrew (Sorex araneus L.) from northern Poland. Experientia 42:960–962. doi:10.​1007/​BF01941780
• Wójcik JM, Borodin PM, Fedyk S, Fredga K, Hausser J, Mishta A, Orlov VN, Searle JB, Volobouev VT, Zima J (2003) The list of chromosome races of the common shrew Sorex araneus (updated 2002). Mammalia 68:169–178
• Wyttenbach A, Hausser J (1996) The fixation of metacentric chromosomes 619 during the chromosomal evolution in the common shrew (Sorex araneus, Insectivora). Hereditas 125:209–217. doi:10.​1111/​j.​1601-5223.​1996.​00209.​x
• Wyttenbach A, Narain Y, Fredga K (1999) Genetic structuring and gene flow in a hybrid zone between two chromosome races of the common shrew (Sorex araneus, Insectivora) revealed by microsatellites. Hereditas 82:79–88. doi:10.​1038/​sj.​hdy.​6884520

Identyfikatory

DOI

10.1007/s13364-011-0029-x