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2011 | 14 | 4 |
Tytuł artykułu

Real-time PCR detection of Mycoplasma felis in domestic cats suffering from chronic conjunctivitis (Poland)

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Real-time PCR directed to intergenic spacer (IGS) noncoding region between 16S and 23S rRNA genes was used for species specific detection of Mycoplasma felis in conjunctival scrapings. Samples were collected from 57 cats suffering from chronic conjunctivitis in 2008-2010 (Wrocław, Poland). Samples from 36 cats (63.2%) were shown to be positive for Mycoplasma felis. Our research gives a first insight in the occurrence of Mycoplasma felis among domestic cats in Poland suggesting that this pathogen may constitute an underestimated cause of chronic conjunctivitis.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
14
Numer
4
Opis fizyczny
p.679-681,fig.,ref.
Twórcy
  • Department of Epizootiology with Clinic for Birds and exotic Animals, Wroclaw University of Environment and Life Sciences, Norwida 31, Wroclaw, Poland
autor
autor
Bibliografia
  • Chalker VJ, Owen WM, Paterson CJ, Brownlie J (2004) Develompent of a polymerase chain reaction for the detection of Mycoplasma felis in domestic cats. Vet Microbiol 100: 77-82.
  • Hartmann AD, Hawley J, Werckenthin C, Lappin MR, Hartmann K (2010) Detection of bacterial and viral organisms from the conjunctiva of cats with conjunctivitis and upper respiratory tract disease. J Feline Med Surg 12: 775-782.
  • Low HC, Powell CC, Veir JK, Hawley JR, Lappin MR (2007) Prevalence of feline herpesvirus 1, Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis. Am J Vet Res 68: 643-648.
  • Maggs DJ (2008) Conjunctiva. In: Maggs DJ, Miller PA, Ofri R (eds) Slatter's Fundamentals of Veterinary Ophthalmology, 4th ed., Saunders Elsevier, St. Louis, pp 135-150.
  • Sandmeyer LS, Waldner CL, Bauer BS, Wen X, Bienzle D (2010) Comparison of polymerase chain reaction tests for diagnosis of feline herpesvirus, Chlamydophila felis, and Mycoplasma spp. infection in cats with ocular disease in Canada. Can Vet J 51: 629-633.
  • Sjödahl-Essen T, Tidholm A, Thorén P, Persson-Wadman A, Bölske G, Aspán A, Berndtsson LT (2008) Evaluation of different sampling methods and results of real-time PCR for detection of feline herpes virus-1, Chlamydophila felis and Mycoplasma felis in cats. Vet Ophthalmol 11: 375-380.
  • Sykes JE (2005) Feline chlamydiosis. Clin Tech Small Anim Pract 20: 129-134.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-6e26a54f-8ab8-459e-9463-135ce70f369e
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