Indirect organogenesis from bud explants of Juniperus communis L.: Effects of genotype, gender, sampling time and growth regulator combinations

• Autorzy: Kocer Z.A. , Gozen A. G. , Onde S. , Kaya Z.
• Języki publikacji: EN

Abstrakty

EN

Common juniper (Juniperus communis L.) is a valuable tree species in forestry and source of many natural products. However, natural regeneration of common juniper is difficult. To develop micropropagation procedures for this species callus induction and organogenesis experiments were carried out. It was found that genotype, gender, sampling time and different growth regulator-combinations had significant effects on callus induction in common juniper. Murashige and Skoog medium supplemented with Benzylaminopurine 0.1 mg l–1and indole-3-butyric acid at concentrations ranging between 0.5–4.0 mg l–1 were the best among the treatments to induce callus formation when spring buds were used as explant sources. In organogenesis experiments, the combination of 2 mg l–1 Benzylaminopurine and 1 mg l–1 2,4-Dichlorophenoxyacetic acid was found to be essential to bring about adventitious bud and shoot development from calli. Genotype, gender and sampling times had significant effects on promoting adventitious bud and shoot development. Although several experimental trials with 0.005, 0.03 and 0.05 mg l–1 indole-3-butyric acid and varying media compositions produced adventitious root like structures, none of these structures further developed into a true root system. However, the results of this pioneering study provide a foundation for further experiments concerning the in vitro regeneration of common juniper.

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2011
• Tom: 66
• Opis fizyczny: p.33-40,fig.,ref.

Twórcy

autor

Kocer Z.A.

autor

Gozen A. G.

autor

Onde S.

autor

Kaya Z.

• Departmnet of Biological Sciences; Middle East Technical University; 06800 Ankara, Turkey

Bibliografia

• Bergmann B.A., Stomp A. 1994. Effect of genotype on in vitro adventitious shoot formation in Pinus radiata and correlations between pairs of phenotypic traits during in vitro shoot development. Plant Cell Tissue and Organ Culture 39: 185–194.
• Bonga J.M., von Aderkas P. 1992. In vitro Culture of Trees. In: Forestry Sciences Vol. 38, Kluwer Publishers, the Netherlands, pp. 2–3 and 74–76.
• Clifton S.J., Ward L.K., Ranner D.S. 1997. The status of juniper Juniperus communis L. in north-east England. Biological Conservation 79: 67–77.
• Confalonieri M., Balestrazzi A., Bisoffi S., Carbonera D. 2003. In vitro culture and genetic engineering of Populus spp.: synergy for forest tree improvement. Plant Cell Tissue and Organ Culture 72: 109–138.
• Diotte M., Bergeron Y. 1989. Fire and the distribution of Juniperus communis L. in the boreal forest of Quebec, Canada. Journal of Biogeography 16: 91-96.
• Ekim T., Koyuncu M., Vural H., Duman H., Aytaç Z., Adýgüzel N. 2000. Red Data Book of Turkish Plants (Pteridophyta and Spermatophyta). Turkish Association for the Conservation of Nature and Van Centennial University, pp. 152.
• Garcia D., Zamora R., Hodar J.A., Gomez J.M. 1999. Age structure of Juniperus communis L. in the Iberian peninsula: Conservation of remnant populations in Mediterranean mountains. Biological Conservation 87: 215–220.
• Garcia D. 2001. Effects of seed dispersal on Juniperus communis recruitment on a Mediterranean mountain. Journal of Vegetation Science 12: 839–848.
• Gomez M.P., Segura J. 1994. Factors controlling adventitious bud induction and plant regeneration in mature Juniperus oxycedrus leaves cultured in vitro In Vitro Cellular and Developmental Biology-Plant 30P: 210–218.
• Gomez M.P., Segura J. 1995a. Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees. Tree Physiology 15: 625–628.
• Gomez M.P., Segura J. 1995b. In vitro control of adventitious bud differentiation by inorganic media components in leaves of mature Juniperus oxycedrus. In Vitro Cellular and Developmental Biology-Plant 31: 179–182.
• Helmersson A., von Arnold S. 2009. Embryogenic cell lines of Juniperus communis; easy establishment and embryo maturation, limited germination. Plant Cell Tissue and Organ Culture 96: 211–217.
• Harry I.S., Pulido C.M., & Thorpe T.A. 1995. Plantlet regeneration from mature embryos of Juniperus cedrus. Plant Cell Tissue and Organ Culture 41: 75–78.
• Kaya Z., Campbell R.K., Adams W.T.1989. Correlated responses of height increment and components of increment in 2-year-old Douglas-fir seedlings. Canadian Journal of Forest Research 19: 1124–1130.
• Kyte L. 1987. Plants from test tubes (Revised Edition) An introduction to micropropagation. Timber Press Inc. Portland. Oregon.
• Lambardi M., Harry I.S., Menabeni D. Thorpe T.A. 1995. Organogenesis and somatic embryogenesis in Cupressus sempervirens. Plant Cell Tissue and Organ Culture 40: 179–182.
• Murashige T., Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–479.
• Negussie A. 1997. In vitro induction of multiple buds in tissue culture of Juniperus excelsa. Forest Ecology and Management 98: 115–123.
• Rout G.R., Samantaray S., Das P. 2000. In vitro manipulation and propagation of medicinal plants. Biotechnology Advances 18: 91–120.
• SAS Inst. Inc. 2002-2003. SAS/STAT User’s Guide. Release 9.1. Edition, Cary, NC. USA.
• Tereso S., Goncalves S., Marum L., Oliveira M., Maroco J., Miguel C. 2006. Improved axillary and adventitious bud regeneration from Portuguese genotypes of Pinus pinaster Ait. Propagation of Ornamental Plants 6: 24–33.
• Tirmenstein D. 1999. Juniperus communis. In: Fire Effects Information System, [Online]. U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Fire Sciences Laboratory (Producer). Available: http://www.fs.fed.us/database/feis/ Cited 22 March 2010.
• Torres K.C. 1989. Tissue culture techniques for horticultural crops. Van Nostrand Reinhold. New York.
• Ward L.K. 1973. The conservation of juniper: present status of juniper in southern England. Journal of Applied Ecology 14: 165–178.
• Yiqun L., Wagner M.R. 1995. Tissue culture of mature ponderosa pine: Callus induction and differentiation. In: Charles van Riper III (ed.). Proceedings of the Second Biennial Conference on Research in Colorado Plateau National Parks. Transactions and proceedings series (United States. National Park Service) — NPS/NRNAU/NRTP-95/11 pp. 225.

Uwagi

rekord w opracowaniu