Utilization of a set of high-polymorphism DAMD markers for genetic analysis of a cucumber germplasm collection

• Autorzy: Hu J.-B. , Li J.-W. , Wang L.-J. , Liu L.-J. , Si S.-W.
• Języki publikacji: EN

Abstrakty

EN

Eighteen minisatellite core sequences, derived from rice, human and phage M13, were used as primers in a PCR technique, known as directed amplification of minisatellite-region DNA (DAMD), to genotype 19 cucumber (Cucumis sativus L.) accessions from a wide collection. All the primers amplified polymorphic bands across the accessions. Out of 165 bands scored, 129 were polymorphic with 78.2% polymorphism. The average of polymorphism information content of the primers was 0.844, revealing a high discrimination power in cucumber. Based on Jaccard’s similarity indices and matrix generated by the DAMD markers, a dendrogram was constructed using the unweighted pair group method using arithmetic averages and allowed for separation of the 19 accessions into four distinct groups which demonstrated genetic relationship among the different types of germplasms. Sequencing of six polymorphic amplicons resulted in the identification of only one minisatellite locus, which indicated that variation in minisatellite number was not always the factor underlying DAMD polymorphism.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 1
• Opis fizyczny: p.227-231,fig.,ref.

Twórcy

autor

Hu J.-B.

• Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China
• Key Laboratory of Fruit Plant and Cucurbit, Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

autor

Li J.-W.

• Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China
• Key Laboratory of Fruit Plant and Cucurbit, Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

autor

Wang L.-J.

• Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China
• Key Laboratory of Fruit Plant and Cucurbit, Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

autor

Liu L.-J.

• Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China
• Key Laboratory of Fruit Plant and Cucurbit, Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

autor

Si S.-W.

• Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China
• Key Laboratory of Fruit Plant and Cucurbit, Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

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Identyfikatory

DOI

10.1007/s11738-010-0525-7