EN
Vaginal discharge from women with acute or chronic trichomonadosis or those infected with Trichomonas asymptomatically was used for examinations. Using Best's method and Schiff's reagent after the treatment with periodic acid, the Trichomonas cytoplasm was found to contain diffused granules of glycogen; Bale's and Steedman's method revealed the presence of acid mucopolysaccharides with hyaluronic acid in the form of granules, accumulated beneath the cell membrane and along the axostyle; the use of Pappenheim-Unna and Brachet's techniques disclosed paranuclear accumulation of ribonucleinic acids; Feulgen's procedure showed the nucleus to contain granules of desoxyribonucleinic acid. No quantitative variations could be assessed in the level of the chemical substances detected in the Protozoa derived from women with various forms of trichomonadosis. The most clear-cut cytologic reactions were seen in the Protozoa from asymptomatic cases of trichomonadosis.