The effect of methyl jasmonate on enzyme activities in wheat genotypes infected by the crown and root rot pathogen Fusarium culmorum

• Autorzy: Motallebi P. , Niknam V. , Ebrahimzadeh H. , Enferadi S.T. , Hashemi M.
• Języki publikacji: EN

Abstrakty

EN

The hemibiotrophic pathogen Fusarium culmorum (Fc) causes crown and root rot (CRR) in wheat. In this study, MeJA treatment was done 6 h after pathogen inoculation (hai) to focus the physiological and biochemical responses in root tissue of the susceptible wheat cv Falat, partially resistant cv Pishtaz and the tolerant cv Sumai3 at the beginning of the necrotrophic stage. The results indicate that treatment with MeJA at 6 hai significantly delayed the necrotic progress in cv Falat, whereas no significant difference was seen in other cultivars. The activities of pathogen responsive defense-related enzymes (SOD, CAT, POX, PPO, LOX and PAL), total phenols and callose contents were higher in Sumai3, while treatment with MeJA significantly increased these enzymes activities and total phenols content in Falat, signifying the most sensitive cultivar which had a weak reaction to the pathogen but a strong response to MeJA treatment. Additionally, MeJA treatment decreased the level of H2O2 and MDA contents particularly in cv Falat. This is the first work reporting the regulation of defense-related enzymes by MeJA treatment at particular time point of 6 hai suggests the possible role of JA in regulating basal resistance in CRR pathogen–wheat interaction. Taken together, our data add new insights into the mechanism of wheat defense including enzymatic events controlling wheat protection against Fc infection.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 11
• Opis fizyczny: Article: 237 [11 p.], fig.,ref.

Twórcy

autor

Motallebi P.

• Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms, School of Biology, College of Science, University of Tehran, 14155-6455, Tehran, Iran

autor

Niknam V.

• Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms, School of Biology, College of Science, University of Tehran, 14155-6455, Tehran, Iran

autor

Ebrahimzadeh H.

• Department of Plant Biology, and Center of Excellence in Phylogeny of Living Organisms, School of Biology, College of Science, University of Tehran, 14155-6455, Tehran, Iran

autor

Enferadi S.T.

• National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e- Pajoohesh, 15th km, Tehran-Karaj Highway, 14965-161, Tehran, Iran

autor

Hashemi M.

• Department of Plant Pathology, Seed and Plant Improvement Institute, Karaj, Iran

Bibliografia

• Abeles FB, Biles CL (1991) Characterization of peroxidase in lignifying peach fruit endocarp. Plant Physiol 95:269–273
• Aebi H (1974) Methods of enzymatic analysis. Academic Press, New York, pp 673–677
• Babitha MP, Prakash HS, Shetty HS (2004) Purification and properties of lipoxygenase induced in downy mildew resistant pearl millet seedlings due to infection with Sclerospora graminicola. Plant Sci 166:31–39
• Backhouse D, Burgess LW (2002) Climatic analysis of the distribution of Fusarium graminearum, F. pseudograminearum and F.culmorum on cereals in Australia. Aust Plant Pathol 31:321–327
• Balali GR, Iranpoor M (2006) Identification and genetic variation of Fusarium species in Isfahan, Iran, Using pectic zymogram technique. Iranian J Sci Technol 30:91–102
• Beccari G, Covarelli L, Nicholson P (2011) Infection processes and soft wheat response to root rot and crown rot caused by Fusarium culmorum. Plant Pathol 60:671–684
• Bell E, Mullet JE (1993) Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding. Plant Physiol 103:1133–1137
• Bradford MM (1976) A dye binding assay for protein. Anal Biochem 72:248–254
• Broukanloui Madloo P, Behboudi K, Tohidfar M, Salehi Jouzani Gh, Ahmadzadeh M (2013) Response of some important Iranian wheat cultivars to Fusarium culmorum under genetic diversity of indigenous bio-control agent Fluorescent pseudomonas spp. Aust J Crop Sci 7(7):1003–1009
• Chaturvedi R, Shah J (2007) Salicylic acid in plant disease resistance. In: Hayat S, Ahmad A (eds) Salicylic acid—a plant hormone. Springer, Dordrecht, pp 335–370
• Chen C, Belanger RR, Benhamou N, Paulitz TC (2000) Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol Mol Plant Pathol 56(1):13–23
• Desmond OJ, Edgar CI, Manners JM, Maclean DJ, Schenk PM, Kazan K (2006) Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiol Mol Plant Pathol 67:171–179
• Devi PUM, Reddy PS, Rani NU, Reddy K, Reddy MN, Reddanna P (2000) Lipoxygenase Metabolites of a-linolenic acid in the development of resistance in pigeonpea, Cajanus cajan (L.) mill sp, seedlings against Fusarium udum infection. Eur J Plant Pathol 106(9):857–865
• Ding L, Xu H, Yi H, Yang L, Kong Z, Zhang L, Xue S, Jia H, Ma Z (2011) Resistance to hemi-biotrophic F. graminearum infection is associated with coordinated and ordered expression of diverse defense signaling pathways. PLoS One 6(4):e19008. doi:10.1371/journal.pone.0019008
• Gadzouska S, Delaunnay A, Spasenoski M, Maury S, Joseph C, Hagege D (2007) Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tiss Org 89(1):1–13
• Giannopolitis CN, Ries SK (1977) Superoxide dismutases I. Occurrence in higher plants. Plant physiol 59(2):309–314
• Glazebrook J (2005) Contrasting mechanism of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol 43:205–227
• Grossman S, Zakut R (1979) Determination of the activity of lipoxygenase (lipoxidase). Methods Biochem Anal 25:303–329
• Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Biol 40(1):347–369
• Heath RL, Packer L (1986) Photoperoxidation in isolated chloroplasts. Arch Biochem Biophys 125:189–198
• Jaiti F, Verdeil JL, Ie Hadrami (2009) Effect of jasmonic acid on the induction of polyphenoloxidase and peroxidase activities in relation to date palm resistance against Fusarium oxysporum f.sp. albedinis. Physiol Mol Plant Pathol 74:84–90
• Jalalpour Z, Shabani L, Afghani L, Sharifi M, Amini SA (2014) Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turk J Biol 38:76–82
• Kavitha R, Umesha S (2008) Regulation of defense-related enzymes associated with bacterial spot resistance in tomato. Phytoparasitica 36(2):144–159
• Kohler A, Schwindling S, Conrath U (2002) Benzothiadiazoleinduced priming for potentiated responses to pathogen infection, wounding, and infiltration of water into leaves require the NPR1/NIM1 gene in Arabidopsis. Plant Physiol 128(3):1046–1056
• Li G, Yen Y (2008) Jasmonate and ethylene signaling pathway may mediate Fusarium head blight resistance in wheat. Crop Sci 48:1888–1896
• Li X, Zhang JB, Song B, Li HP, Xu HQ, Qu B, Dang FJ, Liao YC (2010) Resistance to Fusarium head blight and seedling blight in wheat is associated with activation of a cytochrome P450 gene. Phytopathol 100:183–191
• Madadkhah E, Lotfi M, Nabipour A, Rahmanpour S, Banihashemi Z, Shoorooei M (2012) Enzymatic activities in roots of melon genotypes infected with Fusarium oxysporum f.sp. melonis race 1. Sci Horti 135:171–176
• Makandar R, Nalam VJ, Lee H, Trick HN, Dong Y, Shah J (2012) Salicylic acid regulates basal resistance to Fusarium head blight in wheat. Mol Plant-Microbe Interact 25(3):431–439
• Mandal S, Dahuja A, Santha I (2014) Lipoxygenase activity in soybean is modulated by enzyme-substrate ratio. J Plant Biochem Biotechnol 23(2):217–220
• McKinney HH (1923) Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. J Agri Res 26:195–217
• Mohammadi M, Kazemi H (2002) Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Sci 162:491–498
• Motallebi P, Alkadri D, Pisi A, Nipoti P, Tonti S, Niknam V, Hashemi M, Prodi A (2015) Biomolecular study on Iranian Fusarium culmorum strains on durum wheat and comparison with Italian and Syrian populations. Phyto med. (In Press)
• Ochoa-Alejo N, Gómez-Peralta JE (1993) Activity of enzymes involved in capsaicin biosynthesis in callus tissue and fruits of chili pepper (Capsicum annuum). J Plant Physiol 141(2):147–152
• Petti C, Reiber K, Ali SS, Berney M, Doohan FM (2012) Auxin as a player in the biocontrol of Fusarium head blight disease of barley and its potential as a disease control agent. BMC Plant Biol 12:224
• Raymond J, Pakariyathan N, Azanza JL (1993) Purification and some properties of polyphenol oxidases from some flowers seed. Phytochem 34:927–931
• Reimers PJ, Guo A, Leach JE (1992) Increased activity of a cationic peroxidase associated with an incompatible interaction between Xanthomonas oryzae pv oryzae and rice (Oryza sativa). Plant Physiol 99(3):1044–1050
• Samia M, Khallal EL (2007) Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (Arbuscular mycorrhiza) and/or hormonal elicitors (Jasmonic acid and Salicylic acid) changes in the antioxidant enzymes, phenolic compounds and pathogen related-proteins. Aust J Basic Appl Sci 1(4):717–732
• Scherm B, Balmas V, Spanu F, Pani G, Delogu G, Pasquali M, Migheli Q (2012) Fusarium culmorum: causal agent of foot and root rot and head blight on wheat. Mol Plant Pathol 10:1–19
• Shimada K, Fujikawa K, Yahara K, Nakamura T (1992) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agri Food Chem 40(6):945–948
• Shivakumar PD, Geetha HM, Shetty HS (2002) Peroxidase activity and isozymes analysis of pearl millet seedlings and their implication in downy mildew disease resistance. Plant Sci 160:1–9
• Swain T, Hillis WE (1959) The phenolic constituents of Prumus domestica and quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68
• Ton J, Mauch-Mani B (2004) b-amino-butyric acid-induced resistance against necrotrophic pathogens is based on ABA-dependent priming for callose. Plant J 38(1):119–130
• Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Sci 151(1):59–66
• Verhagen BWM, Glazebrook J, Zhu T, Chang HS, Van Loon LC, Pieterse CMJ (2004) The transcriptome of rhizobacteria induced systemic resistance in Arabidopsis. Mol Plant-Microbe Interact 17:895–908
• Wasternack C, Hause B (2002) Jasmonates and octadecanoids: signals in plant stress responses and development. Prog Nucleic Acid Res Mol Biol 72:165–221
• Wegener M (1992) Optimierung Von Saatgutpillierungen mit mikrobiellen antagonisten zur biologischen Bekampfung Von Fusarium culmorum in weizen. Diplomarbeit, Universitat Gottingen, Gottingen
• Wheatherley PE (1950) Studies in the water relations of cotton plants. I. The field measurement of water deficit in leaves. New Phytol 49:81–87
• Zeyen R, Carver T, Lyngkjaer M, Be´langer R, Bushnell W, Dik A (2002) Epidermal cell papillae. The powdery mildews: a comprehensive treatise. pp 107–125

Identyfikatory

DOI

10.1007/s11738-015-1988-3