The influence of the selected calcium channel blockers on the uterine contraction activity in rats in the in vitro conditions

• Autorzy: Czerski A. , Balcerzak A. , Gnus J. , Wincewicz E. , Zawadzki M. , Zawadzki W.

Warianty tytułu

PL

Wpływ wybranych blokerów kanałów wapniowych na aktywność skurczową macicy szczurów w warunkach in vitro

• Języki publikacji: EN

Abstrakty

EN

The study has been conducted on 20 Bufflo female rats aged 4-6 months, from which the uterus strips have been taken directly after slaughter. Isolated uterus strips were placed in 4 automatic water bath chambers. After recording of spontaneous contraction activity of the isolated strip, calcium channel blockers i.e. diltiazem, nifedypine, verapa­mil were added into the bath organ. Doses of the preparations were determined experi­mentally. The experiments proved that an isolated uterus strip showed spontaneous perio­dical contraction activity for about 8-12 hours. An average contraction frequency amounted to 1.36 ± 0.33 contractions per minute, and strength of a single contraction was on average 2.7 ± 0.7 grams. Administration of oxytocin into the incubation bath organ in­creases the frequency (by 44.85 ± 13.42%) and strength (by 39.5 ± 18.33%) of the sponta­neous uterine contraction activity. Administration of the calcium channel blockers: diltia- zem, nifedypine, verapamil into the incubation bath organ causes a decrease or atony of the frequency and strength of the uterus muscular coat contractions depending on concen­tration of an active substance of the preparation. Nifedypine is the strongest calcium channel blocker. Blocking of the calcium channels with a 2.8 x 10-8 mol/l dose of nifedy­pine resulted in a decrease in frequency by 40 ± 9.86%, whereas a dose ten times higher (2.8 x 10-7) caused total atony of the spontaneous uterine contraction activity. Administra­tion of oxytocin into the incubation bath organ after the prior total blocking of the Ca2+ channels does not result in an increase in the isolated strip contraction activity.

PL

Badania przeprowadzono na 20 szczurzycach linii Bufflo w wieku 4-6 miesięcy, od których pośmiertnie pobrano skrawki macicy. Izolaty montowano w 4 ko­morach automatycznej łaźni wodnej. Po zapisaniu spontanicznej aktywności skurczowej wyizolowanego skrawka do komory podawano blokery kanałów wapniowych: diltiazem, nifedypinę, werapamil. Dawki preparatów ustalano doświadczalnie. W przeprowadzonych badaniach stwierdzono, że wyizolowany skrawek macicy wykazuje spontaniczną, cykliczną kurczliwość przez około 8-12 godzin. Średnia częstotliwość kurczliwości wynosiła 1,36 ± 0,33 skurczu na minutę, a średnia siła skurczu kształtowała się na pozi­omie 2,7 ± 0,7 grama. Podanie do komory inkubacyjnej oksytocyny powoduje wzrost częstotliwości (o 44,85 ± 13,42%) i siły (o 39,5 ± 18,33%) spontanicznej aktywności skurczowej macicy. Podanie do komory inkubacyjnej blokerów kanałów wapniowych: nifedypiny, diltiazemu i werapamilu powoduje spadek lub zanik (atonię) częstotliwości i siły skurczów mięśniówki macicy w zależności od koncentracji substancji czynnej pre­paratu. Najsilniej działającym blokerem kanałów wapniowych jest nifedypina. Zabloko­wanie kanałów wapniowych nifedypiną w dawce 2,8 x 10-8 mol/l powodowało spadek częstotliwości o 40 ± 9,86%, natomiast dawka 10-krotnie wyższa (2,8 x 10-7) powodowała całkowity zanik spontanicznej kurczliwości mięśniówki macicy. Podanie do komory inkubacyjnej oksytocyny po uprzednim całkowitym zablokowaniu kanałów Ca2+ nie powoduje wzrostu aktywności skurczowej wyizolowanego skrawka.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Medicina Veterinaria
• Rocznik: 2009
• Tom: 08
• Numer: 4
• Opis fizyczny: p.33-48,fig.,ref.

Twórcy

autor

Czerski A.

• Department of Animal Physiology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland

autor

Balcerzak A.

• Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

autor

Gnus J.

• Department of General and Vascular Surgery, Regional Specialistic Hospital, Research and Development Centre in Wroclaw, Wroclaw, Poland

autor

Wincewicz E.

• Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

autor

Zawadzki M.

• Department and Clinic of Obsterics, Ruminant Diseases and Animal Health Care, Wroclaw, Poland

autor

Zawadzki W.

• Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

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