Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 68 | 08 |

Tytuł artykułu

Badania nad występowaniem werotoksycznych Escherichia coli w żywności w Polsce

Warianty tytułu

Study of occurrence of verotoxigenic Escherichia coli in food in Poland

Języki publikacji



Verotoxin-producing Escherichia coli (VTEC) strains are enteric bacteria which cause severe consequences of food-borne infections in humans. Most of the illnesses caused by VTEC are attributed to serotype O157:H7 and O157:H-. In recent years the strains belonging to non-O157 serogroups, including the highly virulent E. coli O104:H4 strain which was the cause of an epidemic in Germany in 2011, also have an important significance. The present study shows the examination results of samples from cattle carried out between 2004-2010. The investigations were conducted under the direction of VTEC under two monitoring programs. In the first of them, a total of 1,584 samples collected from bovine carcasses as well as beef meat were tested. In the second study, 406 swabs from carcasses and 417 from beef meat were used. It was found that in the first monitoring program 51 (3.2%) samples possessed the vtx gene. In the second project, from 69 positive samples for VTEC obtained after the first PCR reaction only 29 bacterial isolates were received. These strains were used to determine the serotypes. The most frequent verotoxigenic E. coli belonged to serotypes O181:H49 (n = 4), O2:H[32] (n = 4), as well as serogroups O91 (n = 3) and O113 (n = 3). The detection of VTEC, including E. coli O104:H4, in vegetables was based on the real-time PCR method. The examined material consisted of 443 fresh vegetable samples. The vtx1 and vtx2 genes were not identified, which indicates that the analyzed samples were not contaminated with VTEC strains, including O104:H4 serotype.








Opis fizyczny



  • Zakład Higieny Żywności Pochodzenia Zwierzęcego Państwowego Instytutu Weterynaryjnego - Państwowego Instytutu Badawczego, Al.Partyzantów 57, 24-100 Puławy


  • 1.Arthur T. M., Barkocy-Gallagher G. A., Rivera-Betancourt M., Koohmaraie M.: Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli on carcasses in commercial beef cattle processing plants. Appl. Environ. Microbiol. 2002, 68, 4847-4852.
  • 2.Bae W. K., Lee Y. K., Cho M. S., Ma S. K., Kim S. W., Kim N. H., Choi K. C.: A case of hemolytic uremic syndrome caused by Escherichia coli O104:H4. Yonsei Med. J. 2006, 47, 437-439.
  • 3.Beutin L., Miko A., Krause G., Pries K., Haby S., Steege K., Albrecht N.: Identification of human-pathogenic strains of Shiga toxin-producing Escherichia coli from food by a combination of serotyping and molecular typing of Shiga toxin genes. Appl. Environ. Microbiol. 2007, 73, 4769-4775.
  • 4.Caprioli A., Morabito S., Brugere H., Oswald E.: Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Vet. Res. 2005, 36, 289-311.
  • 5.Cobbold R. N., Davis M. A., Rice D. H., Szymanski M., Tarr P. I., Besser T. E., Hancock D. D.: Associations between bovine, human, and raw milk, and beef isolates of non-O157 Shiga toxigenic Escherichia coli within a restricted geographic area of the United States. J. Food Prot. 2008, 71, 1023-1027.
  • 6.Crowley H., Cagney C., Sheridan J. J., Anderson W., McDowell D. A., Blair I. S., Bishop R. H., Duffy G.: Enterobacteriacae in beef products from retail outlets in the Republic of Ireland and comparison of the presence and counts of E. coli O157:H7 in these products. Food Microbiol. 2011, 22, 409-414.
  • 7.DebRoy C., Fratamico P. M., Roberts E., Davis M. A., Liu Y.: Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups. Appl. Environ. Microbiol. 2005, 71, 4919-4924.
  • 8.DebRoy C., Roberts E., Kundrat J., Davis M. A., Briggs C. E., Fratamico P. M.: Detection of Escherichia coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes. Appl. Environ. Microbiol. 2004, 70, 1830-1832.
  • 9.Decyzja Wykonawcza 2011/402/UE Komisji z dnia 6 lipca 2011 r. w sprawie środków nadzwyczajnych mających zastosowanie do nasion kozieradki i niektórych nasion lub ziaren przywożonych z Egiptu. Dz. U. Unii Europejskiej 2011, L179, 10-12.
  • 10.Detection and identification of Verocytotoxin-producing Escherichia coli (VTEC) O104:H4 in food by Real-time PCR., EU RL Method_food_2Rev.2, 2011.
  • 11.Dyrektywa 2003/99/EC Parlamentu Europejskiego i Rady z dnia 17 listopada 2003 r. w sprawie monitorowania chorób odzwierzęcych i odzwierzęcych czynników chorobotwórczych, zmieniająca decyzję Rady 90/424/EWG i uchylająca dyrektywę Rady 92/117/EWG. Dz. U. Unii Europejskiej 2003, L325, 31-40.
  • 12.Frank C., Werber D., Cramer J. P., Askar M., Faber M., an der Heiden M., Bernard H., Fruth A., Prager R., Spode A., Wadl M., Zoufaly A., Jordan S., Kemper M. J., Follin P., Müller L., King L. A., Rosner B., Buchholz U., Stark K., Krause G., for the HUS investigation team: Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany. N. Engl. J. Med. 2011, 365, 1771-1780.
  • 13.Fratamico P. M., Briggs C. E., Needle D., Chen Ch.-Y., DebRoy C.: Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes. J. Clin. Microbiol. 2003, 41, 3379-3383.
  • 14.Hussein H. S.: Prevalence and pathogenicity of Shiga toxin-producing Escherichia coli in beef cattle and their products. J. Anim. Sci. 2007, 85, 63-72.
  • 15.Maurer J. J., Schmidt D., Petrosko P., Sanchez S., Bolton L., Lee M. D.: Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl. Environ. Microbiol. 1999, 65, 2954-2960.
  • 16.Outbreaks of Escherichia coli O104:H4 infection: update 30. World Health Organization. Geneva 2011.
  • 17.Paton A., Paton J.: Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, entherohemorrhagic E. coli hlyA, rfbO111 and rfbO157. J. Clin. Microbiol. 1998, 36, 598-602.
  • 18.Paton A., Paton J.: Direct detection of Shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157 and O113 by multiplex PCR. J. Clin. Microbiol. 1999, 37, 3362-3365.
  • 19.Paton J., Paton A.: Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 1998, 11, 450-479.
  • 20.Perelle S., Dilasser F., Grout J., Fach P.: Detection by 5'-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world’s most frequent clinical cases. Mol. Cell. Probes 2004, 18, 185-192.
  • 21.Perelle S., Dilasser F., Grout J., Fach P.: Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction. J. Appl. Microbiol. 2005, 98, 1162-1168.
  • 22.Pierard D., Greve H., Haesebrouck F., Mainil J.: O157:H7 and O104:H4 Vero/Shiga toxin-producing Escherichia coli outbreaks: respective role of cattle and humans. Vet. Res. 2012, w druku.
  • 23.Riley L. W., Remis R. S., Helgerson S. D., McGee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargett N. T., Blake P. A., Cohen M. L.: Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 1983, 308, 681-685.
  • 24.Rivera-Betancourt M., Shackelford S. D., Arthur T. M., Westmoreland K. E., Bellinger G., Rossman M., Reagan J. O., Koohmaraie M.: Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella in two geographically distant commercial beef processing plants in the United States. J. Food Prot. 2004, 67, 295-302.
  • 25.Scheutz F., Møller Nielsen E., Frimodt-Møller J., Boisen N., Morabito S., Tozzoli R., Nataro J. P., Caprioli A.: Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011. Euro Surveill. 2011, 16, pii=19889. Available online:
  • 26.Scientific Opinion of the Panel on Biological Hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic VTEC types. EFSA Journal 2007, 579, 1-61.
  • 27.Shiga toxin/verotoxin-producing Escherichia coli in humans, food and animals in the EU/EEA, with special reference to the German outbreak strain STEC O104. Technical Report. European Centre for Disease Prevention and Control and European Food Safety Authority. Stockholm 2011.
  • 28.Sobieszczańska B. M., Gryko R., Malek C. W.: Isolation of verotoxigenic strains of Escherichia coli O26 in Poland. Clin. Microbiol. Infect. 2006, 6, 227-229.
  • 29.Szych J., Paciorek J., Cieślik A, Kałużewski S.: Charakterystyka szczepów E. coli O157 izolowanych w Polsce z próbek materiału klinicznego i z żywności. Med. Dośw. Microbiol. 1999, 50, 179-196.
  • 30.The European Union Summary Report on Trends and Sources of Zoonoses and Zoonotic Agents and Food-borne Outbreaks in 2009. EFSA Journal 2011, 9, 1-378.
  • 31.Wieczorek K., Beutin L., Osek J.: Rare VTEC serotypes of potential zoonotic risk isolated from bosine hides and carcases. Vet. Rec. 2011, 168, 80-81.
  • 32.Wieczorek K., Osek J.: Simultaneous occurrence of selected food-borne bacterial pathogens on bovine hides, carcasses and beef meat. Pol. J. Vet. Sci. 2010, 13, 645-651.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.