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2015 | 18 | 3 |

Tytuł artykułu

Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Lactoferrin (Lf) gene promoter was screened for the presence of single nucleotide polymphism in indigenous and crossbred cattle from North India and to evaluate its association with Mastitis. Study revealed the presence of genetic variation in regulatory region of bovine Lactoferrin gene using PCR-RFLP technique. Three genotypes namely GG, GH and HH were identified. A single nucleotide change, from guanine to adenine at 25th position was found to be significantly associated (p<0.05) with clinical mastitis in indigenous Sahiwal and crossbred Karan Fries cattle maintained at organised herd of National Dairy Research Institute, Karnal. A non-significant association was observed between subclinical mastitis, somatic cell score (SCS), and GG genotype in Karan Fries cattle, however, a lower SCS was observed in animals having GG genotype. Overall a lower incidence of clinical mastitis was recorded in those animals having GG genotype of Lf in Sahiwal and Karan Fries (KF) cattle. The SNP identified in the promoter region may effect expression lactoferrin protein, which may lead to different levels of antibacterial and anti- nflammatory activity of Lf gene. Results from this study indicated the probable role played by Lactoferrin promoter to serve as candidate gene for mastitis susceptibility among indigenous and crossbred milch cattle.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

18

Numer

3

Opis fizyczny

p.465-471,fig.,ref.

Twórcy

autor
  • ICAR - National Dairy Research Institute, Karnal – 132001 (Haryana), India
autor
  • ICAR - National Dairy Research Institute, Karnal – 132001 (Haryana), India
autor
  • ICAR - National Dairy Research Institute, Karnal – 132001 (Haryana), India
  • ICAR - National Dairy Research Institute, Karnal – 132001 (Haryana), India
autor
  • ICAR - National Bureau of Animal Genetic Resources, Karnal – 132001 (Haryana), India

Bibliografia

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  • Bruckmaier RM (2005) Gene expression of factors related to the immune reaction in response to intramammary Escherichia coli lipopolysaccharide challenge. J Dairy Res 72: 120-124.
  • Changhong Zhao, Gaoming He Yanliang Wang, Zhaoxia, Zhang (2009) Polymorphism of lactoferrin gene with PCR – RFLP and its association with subclinical mastitis in dairy cows. Modern Applied Sci 3: 144-148.
  • Chopra A, Gupta ID, Verma A, Vohra V (2014) Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis. Indian J Anim Sci 84: 1068-1070.
  • Concha C (1986) Cell types and their immunological functions in bovine mammary tissues and secretions- a review of the literature. Nord Vet Med 38: 257-272.
  • Daly M, Ross P, Giblin L, Buckley F (2006) Polymorphisms within the lactoferrin gene promoter in various cattle breeds. Anim Biotechnol 17: 33-42.
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  • Harvey WR (1987) Least-squares analysis of data with unequal sub-class numbers. Agricultural research services, USDA, USA.
  • Huang J, Wang H, Wang C, Li J, Li Q, Hou M, Zhong J (2010) Single nucleotide polymorphisms, haplotypes and combined genotypes of lactoferrin gene and their associations with mastitis in Chinese Holstein cattle. Mol Biol Rep 37: 477-483.
  • Li GH, Zhang Y, Sun DX, Li N (2004) Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis. Anim Biotechnol 15: 67-76.
  • Martin-Burriel I, Osta R, Barendse W, Zaragoza P (1997) New polymorphism and linkage mapping of the bovine lactotransferrin gene. Mamm Genome 8: 704-705.
  • Molenaar AJ, Kuys YM, Davis SR, Wilkins RJ, Mead PE, Tweedie JW (1996) Elevation of lactoferrin gene expression in developing, ductal, resting, and regressing parenchymal epithelium of the ruminant mammary gland. J Dairy Sci 79: 1198-1208.
  • Sharma N, Kang TY, Lee S, Kim JN, Hur CH, Ha JC, Vohra V, Jeong DK (2013) Status of bovine mastitis and associated risk factors in subtropical Jeju Island, South Korea. Trop Anim Health Prod 45: 1829-1832.
  • Rahmani SF, Jayashankar MR, Nagaraja CS, Bhaskaran R, Byregowda SM, Satyanarayan K (2012) Association of genotypes at promoter region of lactoferrin gene with mastitis in crossbred cattle. Indian J Anim Sci 82: 718-720.
  • Sambrook J and Russel DW (2001) Molecular Cloning: A laboratory manual. 3rd ed. vol 1., Cold Spring Harbor Laboratory press. New York, USA.
  • Schwerin M, Solinas Toldo S, Eggen A, Brunner R, Seyfert HM, Fries R (1994) The bovine lactoferrin gene (LTF) maps to chromosome 22 and synthetic group U12. Mam Genome 5: 486-489.
  • Seyfert HM, Henke M, Interthal H, Klussmann H, Koczan D, Natour S, Pusch W, Senft B, Steinhoff UM, Tuckoricz A, Hobom G (1996) Defining candidate genes for mastitis resistance in cattle: the role of lactoferrin and lysozyme. J Anim. Breed. Genet 113: 269-276.
  • Zabolewicz T, Barcewicz M, Brym P, Puckowska P, Kamiński S (2014) Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows. Pol J Vet Sci 17: 633-641.
  • Wojdak-Maksymie K, Kmiec M, Ziemak J (2006) Associations between bovine lactoferrin gene polymorphism and somatic cell count in milk. Vet Med 51: 14-20.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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