Fine-tuned MMP-9 activity level is a premissive factor for induction and maintenance of LTP in hippocampal mossy fiber-CA3 pathway

• Autorzy: Wiera G. , Dziegiel P. , Kaczmarek L. , Mozrzymas J. W.
• Języki publikacji: EN

Abstrakty

EN

Matrix metalloproteinases have been implicated in physiological and pathological functions of neuronal networks. To get an insight into the specific role of protease MMP-9 in the plasticity of the mossy fiber-CA3 pathway (mf-CA3), we used field recordings to measure LTP in slices from wild type, MMP-9 KO and overexpressing (OVX) animals. Both lack of protease (KO) and its overexpression impaired the early and maintenance phase of mf-CA3 LTP (128% vs. 181% of respective baseline in KO and WT mice; and 136% vs. 189% in OVX and WT rats respectively, P<0.05). Importantly, impaired LTP in transgenic rodents could be rescued by administration of recombinant MMP-9 to the KO slices (152% of respective baseline). Moreover, autoactive MMP-9 protein application induced a stable autopotentiation of synaptic response in KO and WT mice. β-dystroglycan is a synaptic membrane protein and its MMP-mediated cleavage has been described during pathological, epileptic activity. In mf-CA3 pathway we observed a MMP-dependent significant decrease in 30 kDa β-dystroglycan digestion product as early as 5 minutes after LTP induction. Altogether, these results suggest that in mfCA3 pathway during first minutes after tetanus fine-tuned level of MMP-9 activity acts as a permissive factor for LTP establishment. Supported by Polish Ministry for Science and Higher Education grant N N401 541540.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2012
• Tom: 72
• Numer: 2
• Opis fizyczny: p.204

Twórcy

autor

Wiera G.

• Wroclaw Medical University, Wroclaw, Poland
• Wroclaw University, Wroclaw, Poland

autor

Dziegiel P.

• Wroclaw University, Wroclaw, Poland

autor

Kaczmarek L.

• Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Mozrzymas J. W.

• Wroclaw Medical University, Wroclaw, Poland
• Wroclaw University, Wroclaw, Poland

Uwagi

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