Molecular variability of the coat protein gene of Prunus necrotic ringspot virus isolates

• Autorzy: Sala-Rejczak K. , Paduch-Cichal E.

Warianty tytułu

PL

Zróżnicowanie genetyczne genu kodującego białko płaszcza izolatów Prunus necrotic ringspot virus

• Języki publikacji: EN

Abstrakty

EN

We have obtained and described the nucleotide sequences corresponding to ilarvirus Prunus necrotic ringspot virus (PNRSV] coat protein (CP) gene in eleven Polish isolates, from different Prunus and Rosa species and four isolates provided in plant material from Australia, Hungary and Italy. Virus identification was possible using specific primers allowed amplifying the 700 bp amplicon of coat protein gene. The product was obtained used IC-RT-PCR. The results indicated no association between the host species or the geographic origin and the PNRSV CP sequence specificity. The CP gene nucleotide sequence of studied isolates allowed for clustering them into the previously reported PV32-I, PV96-II and PE5-III phylogroups.

PL

W pracy uzyskano i opisano sekwencje genu kodującego białko płaszcza 11 izolatów wirusa nekrotycznej pierścieniowej plamistości wiśni (Prunus necrotic ringspot virus, PNRSV) z różnych gatunków roślin z rodzaju Prunus i Rosa pochodzących z Polski oraz czterech izolatów wirusa uzyskanych w materiale roślinnym z Australii, Węgier i Włoch. Wykorzystując specyficzne startery, uzyskano produkt 700 par zasad obejmujący gen kodujący białko płaszcza wirusa. Do amplifikacji zastosowano technikę IC-RT-PCR. Wykazano brak korelacji pomiędzy gatunkiem, pochodzeniem izolatu, a sekwencją nukleotydów białka jego płaszcza. Sekwencje genu kodującego CP badanych izolatów przyporządkowano do trzech głównych filogenetycznych grup wzorcowych: PV32-I, PV96-II i PE5-III.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2013
• Tom: 12
• Numer: 2
• Opis fizyczny: p.35-42,ref.

Twórcy

autor

Sala-Rejczak K.

• Department of Plant Pathology, Warsaw University of Life Sciences WULS - SGGW, ul.Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Paduch-Cichal E.

• Department of Plant Pathology, Warsaw University of Life Sciences WULS - SGGW, ul.Nowoursynowska 159, 02-776 Warsaw, Poland

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