HUCB-NSCS growth control by biofunctionalized surfaces

• Autorzy: Zychowicz M. , Ruiz A. , Cerioti L. , Lisboa P. , Mehn D. , Coecke S. , Rausher H. , Colpo P. , Rossi F. , Domanska-Janik K. , Buzanska L.
• Języki publikacji: EN

Abstrakty

EN

Cell growth platforms with biofunctionalized surfaces were fabricated to control and direct HUCB-NSC fate decisions. Two different nano/micro techniques: microcontact printing and piezoelectric non-contact spotting were used to allocate biomolecules (poly-Llysine and fi bronectin) on cell-repellent, non adhesive substrate. Such methods allow controlling the spatial distribution and content of the biomolecules on the microarray and governing cell adhesion in unspecifi c (electrostatic) or specifi c (receptor-mediated) manner. Patterning of biomolecules in different conditions on the single growth platforms enables to infl uence and compare stem cells developmental processes (proliferation /differentiation) at variable environments. To refl ect/mimic stem cell niche we applied functional domains containing ECM protein spotted together with the small signaling molecules (notch, wnt, shh). Such approach enable directing of neural stem cell developmental program by inducing intracellular molecular pathways leading to either self renewing- or differentiating- (neuronal or astrocytic) state. We characterized active biofunctionalized domains on fabricated microarrays by applying two methods of surface analysis: ellipsometry measurement and surface plasmon resonance system. This type of bioengineered cell growth platforms can be used for screening the mechanisms governing neural stem cell fate decisions and adverse reactions upon environmental stimuli. Grant No 0141/B/ P01/2008/35.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.294

Twórcy

autor

Zychowicz M.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Ruiz A.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Cerioti L.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Lisboa P.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Mehn D.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Coecke S.

• In Vitro Toxicology Methods Unit, Institure fo Health and Consumer Protection, European Commission, Joint Research Centre, Ispra, Italy

autor

Rausher H.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Colpo P.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Rossi F.

• Nanobiosciences Unit, Joint Research Centre, Ispra, Italy

autor

Domanska-Janik K.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Buzanska L.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland