The cellular pathway and enzymatic activity for phloem-unloading transition in developing Camellia oleifera Abel. Fruit

• Autorzy: Wang X. , You H. , Yuan Y. , Zhang H. , Zhang L.
• Języki publikacji: EN

Abstrakty

EN

The phloem-unloading pathway of sucrose and mechanism of sugar-to-oil transition are still unknown in Camellia oleifera Fruit. Here, transmission electronic microscopy (TEM) and confocal laser-scanning microscopy (CLSM) were used to observe the cellular structure of vascular bundles and symplastic tracer, carboxyfluorescein (CF), transport in phloem zone. The results showed that sucrose was transported via symplast system in the early and late phases, whereas apoplast system exerted the function in middle stage. Moreover, enzymatic assays showed that acid invertase had a higher activity at the transition stage during the whole fruit development. The cell wall bound invertase (CWI) activity reached the highest at the middle stage of fruit development and the switch in phloem-unloading coincided with fruit developmental phase change and oil accumulation. Correlation analysis showed that the oil accumulation was significantly negatively correlated with content of soluble sugar at P < 0.05 level. However, the soluble acid invertase (SAI), CWI, and neutral invertase showed a significant positive correlation with oil accumulation at P < 0.01 level. In summary, our data provide new cytological insights into the transition of unloading transfer between symplasmic and apoplasmic patterns in C. oleifera fruit and suggest that invertases are positively involved in sugar–oil transition process.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2018
• Tom: 40
• Numer: 02
• Opis fizyczny: Article 23 [11p.], fig.,ref.

Twórcy

autor

Wang X.

• Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China

autor

You H.

• Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China

autor

Yuan Y.

• Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China

autor

Zhang H.

• Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China

autor

Zhang L.

• Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China

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Identyfikatory

DOI

10.1007/s11738-017-2598-z