Antioxidant response to drought in red white clover

• Autorzy: Vaseva I. , Akiscan Y. , Simova-Stoilova L. , Kostadinova A. , Nenkova R. , Anders I. , Feller U. , Demirevska K.
• Języki publikacji: EN

Abstrakty

EN

Antioxidant response to drought in red (Trifolium pratense L., cv. ‘‘Start’’) and white clover (Trifolium repens L, cv. ‘‘Haifa’’ and cv. ‘‘Debut’’) grown as soil cultures was evaluated in water-deprived and recovered plants. Drought provoked oxidative stress in leaves confirmed by the considerable changes in electrolyte leakage, malondialdehyde, hydrogen peroxides and proline contents. Immunoblot of Δ-1-pyrroline-5-carboxylate synthetase (P5CS), which catalyzes the first two steps in proline biosynthesis, revealed strong induction of the enzyme in red clover plants submitted to drought. Water-deprived white clover plants exhibited distinct P5CS profiles. This was related to different drought tolerance of the studied T. repens cultivars. Isoenzyme analyses of superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) demonstrated certain differences in antioxidant defence among the tested varieties. It was confirmed that MnSOD (in both T. repens and T pratense) and FeSOD (in T. repens) isoforms were the most affected by drought. The red clover cultivar ‘‘Start’’ exhibited the lowest FeSOD and POX activities which could contribute to its poor performance under water deprivation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 5
• Opis fizyczny: p.1689-1699,fig.,ref.

Twórcy

autor

Vaseva I.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

autor

Akiscan Y.

• Department of Field Crops, Faculty of Agriculture, Mustafa Kemal University, 31120 Antakya, Hatay, Turkey

autor

Simova-Stoilova L.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

autor

Kostadinova A.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

autor

Nenkova R.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

autor

Anders I.

• Institute of Plant Sciences and Oeschger Centre for Climate Change Research (OCCR), University of Bern, Altenbergrain 21, 3013 Bern, Switzerland

autor

Feller U.

• Institute of Plant Sciences and Oeschger Centre for Climate Change Research (OCCR), University of Bern, Altenbergrain 21, 3013 Bern, Switzerland

autor

Demirevska K.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

Bibliografia

• Abass SM, Mohamed HI (2011) Alleviation of adverse effects of drought stress on common bean (Phaseolus vulgaris L.) by exogenous application of hydrogen peroxide. Bangladesh J Bot 41(1):75–83
• Acar O, Tiirkan I, Ozdemir F (2001) Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgate L.) varieties. Acta Physiol Plant 23(3): 351–356
• Alia PM, Matysik J (2001) Effect of proline on the production of singlet oxygen. Amino Acids 21:195–200
• Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutase (SODs) in controlling oxidative stress in plants. Exp Bot 53:133–141
• Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
• Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396
• Bailly C, Benamar A, Corbineau F, Dome D (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seed as related to deterioration during accelerated aging. Physiol Plant 97:104–110
• Bajji M, Kinet JM, Lutts S (2002) The use of electrolyte leakage method for assessing cell membrane stability as water stress tolerance test in durum wheat. Plant Growth Regul 36:61–70
• Bandurska H (2000) Does proline accumulated in leaves of water deficits stressed barley plants confine cell membrane injury? I. Free proline accumulation and membrane injury index in drought and osmotically stressed plants. Acta Physiol Plant 22:409–415
• Bates LS, Waldren RO, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
• Bermejo R, Irigoyen JJ, Santamarıa JM (2006) Short-term drought response of two white clover clones, sensitive and tolerant to O₃. Physiol Plant 127:658–669
• Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194
• Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Cázares BX, Ortega FAR, Elenes LF, Medrano RR (2010) Drought tolerance in crop plants. Am J Plant Physiol 5:241–256
• Chaves MM, Maroco JP, Pereira JS (2003) Understanding plant responses to drought—from genes to whole plant. Funct Plant Biol 30:239–264
• Chen JB, Wang SM, Jing RL, Mao XG (2009) Cloning the PvP5CS gene from common bean (Phaseolus vulgaris) and its expression patterns under abiotic stresses. J Plant Physiol 166(1):12–19
• Cruz de Carvalho MH (2008) Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signal Behav 3(3):156–165
• Cushman JC, DeRocher EJ, Bohnert HJ (1990) Gene expression during adaptation to salt stress. In: Katterrman F (ed) Environmental Injury to Plants. Academic, San Diego, pp 173–203
• Dat J, Vandenbeele S, Vranova E, Van Montagu M, Inzé D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
• Edjolo A, Laffray D, GuerrierG(2001) The ascorbate-glutathione cycle in the cytosolic and chloroplastic fractions of drought-tolerant and drought-sensitive poplars. J Plant Physiol 158:1511–1517
• Edmeades DC, Blamey FPC, Asher CJ, Edwards DG (1991) Effect of pH and aluminium on the growth of temperate pasture species: II. Growth and nodulation of legumes. Aust J Agric Res 42:893–900
• Erdei L, Tari I, Csiszar J, Pecsvaradi A, Horvath F, Szabo M, Ordog M, Cseuz L, Zhiponova M, Szilak L, Gyorgyey J (2002) Osmotic stress responses of wheat species and cultivars differing in drought tolerance: 20 some interesting genes (advices for gene hunting). Acta Biol Szegediensis 46:63–65
• Esfandiari E, Shakiba MR, Mahboob S, Alyari H, Toorchi M (2007) Water stress, antioxidant enzyme activity and lipid peroxidation in wheat seedling. J Food Agric Environ 5:149–153
• Falaknaz M, Heidari P, Mehrabi AA, Kahrizi D, Yari K (2011) Phylogeny of D-1-pyrroline-5-carboxylate synthase (P5CS) gene in differential spices of plant using cDNA AFLP analysis. Am J Sci Res 19:13–21
• Foyer CH, Noctor G (2009) Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications. Antioxid Redox Signal 11(4):862–878
• Fu J, Huang B (2001) Involvement of antioxidants and lipid peroidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45:105–114
• Fujita T, Maggio A, Garcia-Rios M, Bressan RA, Csonka LN (1998) Comparative analysis of the regulation of expression and structures of two evolutionarily divergent genes for Δ-1-pyrroline-5-carboxylate synthetase from tomato. Plant Physiol 118:661–674
• Ginzberg I, Stein H, Kapulnik Y, Szabados L, Strizhov N, Schell J, Koncz C, Zilberstein A (1998) Isolation and characterization of two different cDNAs of Δ-1-pyrroline-5-carboxylate synthase in alfalfa, transcriptionally induced upon salt stress. Plant Mol Biol 38:755–764
• González A, Steffen KL, Lynch JP (1998) Light and excess manganese. Implications for oxidative stress in common bean. Plant Physiol 118:493–504
• Guo Z, Ou W, Lu S, Zhong Q (2006) Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem 44:828–836
• Halliwell B, Gutteridge JMC (1989) Free radicals in biology and medicine, 2nd edn. Clarendon Press, Oxford
• Hamdy A, Ragab R, Scarascia-Mugnozza E (2003) Coping with water scarcity: water saving and increasing water productivity. Irrig Drain 52:3–20
• Hanson AD, Hitz WD (1982) Metabolic responses of mesophytes to plant water deficits. Ann Rev Physiol 33:163–203
• Hart MA, Tyson H, Bloomberg B (1971) Measurement of activity of peroxidase isoenzymes in flax. Can J Bot 49:2129–2137
• He L, Gao Z, Li E (2009) Pretreatment of seed with H₂O₂ enhances drought tolerance of wheat (Triticum aestivum L.) seedlings. Afri J Biotech 8(22):6151–6157
• Hmida-Sayari A, Gargouri-Bouzid R, Bidani A, Jaoua L, Savourè A, Jaoua S (2005) Overexpression of Δ-1-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants. Plant Sci 169:746–752
• Hodges DM, de Long JM, Forney ChF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611
• Hoekstra FA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccation tolerance. Trends Plant Sci 6:431–438
• Hong Z, Lakkineni K, Zhang Z, Verma DPS (2000) Removal of feedback inhibition of Δ¹-Pyrroline-5-Carboxylate Synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol 122:1129–1136
• Hung SH, Yu CW, Lin CH (2005) Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin 46:1–10
• Hur J, Jung KH, Lee CH, An GH (2004) Stress-inducible OsP5CS2 gene is essential for salt and cold tolerance in rice. Plant Sci 167:417–426
• Ishibashi Y, Yamaguchi H, Yuasa T, Iwaya-Inoue M, Arima S, Zheng SH (2011) Hydrogen peroxide spraying alleviates drought stress in soybean plants. J Plant Physiol 168(13):1562–1567
• Jaeger EB, Seneviratne SI (2011) Impact of soil moisture–atmosphere coupling on European climate extremes and trends in a regional climate model. Clim Dyn 36:1919–1939
• Jain M, Mathur G, Koul S, Sarin NB (2001) Ameliorative effects of proline on salt stress-induced lipid peroxidation in cell lines of groundnut (Arachis hypogaea L.). Plant Cell Rep 20:463–468
• Kishor KPB, Hong Z, Miao CH, Hu CAA, Verma DPS (1995) Overexpression of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394
• Kishor KPB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao SS, Rao S, Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438
• Koleva E, Alexandrov V (2008) Drought in the Bulgarian low regions during the 20th century. Theor Appl Clim 92:113–120
• Laloi Ch, Apel K, Danon A (2004) Reactive oxygen signalling: the latest news. Curr Opin Plant Biol 7:323–328
• Lascano HR, Antonicelli GE, Luna CM, Melchiorre MN, Gomez LD, Racca RW, Trippi VS, Casano LM (2001) Antioxidant system response of different wheat cultivars under drought: field and in vitro studies. Aust J Plant Physiol 28:1095–1102
• Lee BR, Kim KY, Jung WJ, Avice JC, Ourry A, Kim TH (2007) Peroxidases and lignification in relation to the intensity of waterdeficit stress in white clover (Trifolium repens L.). J Exp Bot 58:1271–1279
• Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol 119:1091–1099
• Malaviya DR, Roy AK, Kaushal P, Kumar B, Tiwari A (2008) Genetic similarity among Trifolium species based on isozyme banding pattern. Plant Syst Evol 276:125–136
• Martinez CA, Loureiro ME, Oliva MA, Maestrip M (2001) Differential responses of superoxide dismutase in freezing resistant Solanum curtilobum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Sci 160:505–515
• Mitsuhashi W, Feller U (1992) Effects of light and external solutes on the catabolism of nuclear-encoded stromal proteins in intact chloroplasts isolated from pea leaves. Plant Physiol 100:2100–2105
• Mittler R (2002) Oxidative stress, antioxidants, and stress tolerance. Trends Plant Sci 7:405–410
• Neill SJ, Desikan R, Clarke A, Hurst RD, Hancock JT (2002) Hydrogen peroxide and nitric oxide as signalling molecules in plants. J Exp Bot 53(372):1237–1247
• Noctor G, Veljovic-Jovanovic S, Driscoll S, Novitskaya L, Foyer CH (2002) Drought and oxidative load in the leaves of C₃ plants: a predominant role for photorespiration? Ann Bot 89:841–850
• Nunes MES, Smith GR (2003) Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Sci 43:1349–1357
• Pérez-Arellano I, Carmona-Alvarez F, Martínez AI, Rodríguez-Díaz J, Cervera J (2010) 1,2, Pyrroline-5-carboxylate synthase and proline biosynthesis: From osmotolerance to rare metabolic disease (Review). Prot Sci 19:372–382
• Reddy RA, Chaitanya KV, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202
• Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plan Physiol 101(1):7–12
• Seneviratne SI, Lüthi D, Litschi M, Schär C (2006) Land–atmosphere coupling and climate change in Europe. Nature 443:205–209
• Simova-Stoilova L, Demirevska K, Kingston-Smith A, Feller U (2012) Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Sci 183(7):43–49
• Ślesak I, Libik M, Karpinska B, Karpinski S, Miszalski Z (2007) The role of hydrogen peroxide in regulation of plant metabolism and cellular signalling in response to environmental stresses. Acta Biochem Pol 54(1):39–50
• Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057–1060
• Strizhov N, Ábrahám E, Ökresz L, Blickling S, Zilberstein A, Schell J, Koncz C, Szabados L (1997) Differential expression of two P5CS genes controlling proline accumulation during salt-stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis. Plant J 12:557–569
• Szèkely G, Abraham E, Cseplo A, Rigo G, Zsigmond L, Csiszar J, Ayaydin F, Strizhov N, Jasik J, Schmelzer E, Koncz C, Szabados L (2008) Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis. Plant J 53:11–28
• Turchetto-Zolet AC, Margis-Pinheiro M, Margis R (2009) The evolution of pyrroline-5-carboxylate synthase in plants: a key enzyme in proline synthesis. Mol Genet Genom 281:87–97
• Türkan İ, Bor M, Özdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of droughttolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231
• Vaseva I, Akiscan Y, Demirevska K, Anders I, Feller U (2011) Drought stress tolerance of red and white clover—comparative analysis of some chaperonins and dehydrins. Sci Hortic 130(3):653–659
• Verdoy D, De la Pena TC, Redondo FJ, Lucas MM, Pueyo JJ (2006) Transgenic Medicago truncatula plants that accumulate proline display nitrogen-fixing activity with enhanced tolerance to osmotic stress. Plant Cell Environ 29:1913–1923
• Voigt PW, Mosjidis JA (2002) Acid-soil resistance of forage legumes as assessed by a soil-on-agar method. Crop Sci 42:1631–1639
• Wang ChQ, Chang LR (2008) Enhancement of superoxide dismutase activity in the leaves of white clover (Trifolium repens L.) in response to polyethylene glycol-induced water stress. Acta Physiol Plant 30:841–847
• Wang ChQ, Zhang YF, Zhang YB (2008) Scavenger enzyme activities in subcellular fractions of white clover (Trifolium repens L.) under PEG-induced water stress. J Plant Growth Regul 27:387–393
• Wolff SP (1994) Ferrous ion oxidation in presence of ferric ion indicator xylenolorange for measurement of hydroperoxides. In: Packer L (ed) Methods in enzymol 233. Academic Press, New York, pp 182–189
• Woodbury W, Spencer AK, Stahmann MA (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal Biochem 44:301–305
• Yu J, Mosjidis JA, Klinger KA, Woods FM (2001) Isozyme diversity in North American cultivated red clover. Crop Sci 41:1625–1628
• Zhang J, Kirkham MB (1996) Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol 132:361–373

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