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2013 | 60 | 4 |

Tytuł artykułu

Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Leptin and leptin receptor genes are considered as production traits markers in dairy or beef cattle. The aim of this study was to verify the associations of polymorphisms in bovine LEP and LEPR genes with production and reproduction traits in Slovak Spotted and Pinzgau cows. Long-life production was evaluated: milk, protein, and fat yield and reproduction traits: age at first calving, calving interval, days open, and insemination interval. In total, 296 blood samples of Slovak Spotted and 85 hair roots samples of Pinzgau cows were analyzed. In order to detect LEP/Sau3AI (BTA 4, inron 2) and LEPR/T945M (BTA 3, exon 20) genotypes PCR-RFLP method was used. In Slovak Spotted and Pinzgau cows allele frequencies were 0.838/0.162 and 0.694/0.306 for A and B LEP variants, and 0.954/0.046 and 0.912/0.088 for C and T LEPR variants, respectively. For testing the associations between SNPs LEP/Sau3AI and LEPR/T945M and evaluated traits, the General Linear Model procedure in SAS Software was used. Statistical analysis showed that SNP LEP/Sau3AI significantly affected milk, protein and fat yield (P<0.05), and age at first calving (P<0.01) in analyzed population of cows. Statistically, SNP LEPR/T945M affected significantly calving interval (P<0.01) only. Results of our study suggest that especially leptin is a candidate gene, which influences mainly milk production traits and might be implemented in breeding strategies to improve the production performance of both analyzed cattle breeds.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

60

Numer

4

Opis fizyczny

p.783-787,ref.

Twórcy

  • Department of Animal Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, The Slovak Republic
  • Department of Animal Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, The Slovak Republic
autor
  • Department of Animal Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, The Slovak Republic

Bibliografia

  • Almeida SEM, Almeida EA, Moraes JCF, Weimer TA (2003) Molecular markers in the LEP gene and reproductive performance of beef cattle. J Anim Breed Genet 12: 106-113.
  • Almeida SEM, Santos LBS, Passos DT, Corbellini AO, Lopes BMT, Kirst C, Terra G, Neves JP, Goncalves PBD, Moraes JCF, Azevedo Weimer T (2008) Genetic polymorphsims at the leptin receptor gene in three beef cattle breeds. Genet Mol Biol 31: 680-685.
  • Anton I, Kovács K, Holló G, Farkas V, Szabó F, Egerszegi I, Rátky J, Zsolnai A, Brüssow KP (2012) Effect of DGAT1, leptin and TG gene polymorphisms on some milk production traits in different dairy cattle breeds in Hungary. Arch Tierz 55: 307-414.
  • Boelhauve M, Sinowatz F, Wolf E, Paula-Lopes FF (2005) Maturation of bovine oocytes in the presence of leptin improves development and reduces apoptosis of in vitro-produced blastocysts. Biol Reprod 73: 737-744. 
  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphism. Amer J Hum Genet 32: 314-331. 
  • Chebel RC, Santos JE (2011) Association between leptin single nucleotide polymorphism and reproductive performance of lactating Holstein cows. Anim Reprod Sci 127: 126-134. 
  • Clempson AM, Pollott GE, Brickell JS, Bourne NE, Munce N, Wathes DC (2011) Evidence that leptin genotype is associated with fertility, growth, and milk production in holstein cows. J Dairy Sci 94: 3618-3628. 
  • Cunningham MJ, Clifton DK, Steiner RA (1999) Leptin's actions on the reproductive axis: perspectives and mechanisms. Biol Reprod 60: 216-222. 
  • Forhead AJ, Fowden AL (2009) The hungry fetus? Role of leptin as a nutritional signal before birth. J Physiol 587: 1145-1152. 
  • Glantz M, Lindmark Månsson H, Stålhammar H, Paulsson M (2012) Effect of polymorphisms in leptin, leptin receptor and acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition. J Dairy Res 79: 110-118. 
  • Glantz M, Lindmark Månsson H, Stålhammar H, Paulsson M (2012) Effect of polymorphisms in leptin, leptin receptor and acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition. J Dairy Res 79: 110-118. 
  • Komisarek J (2010) Imapct of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle. Anim Sci Pap Rep 28: 133-141.
  • Kulig H, Kmieć M (2009) Association between leptin Gene Polymorphisms and growth Traits in Limousin Cattle. Genetika 45: 838-841. 
  • Kulig H, Kmieć M, Wojdak-Maksymiec K (2010) Associations between Leptin Gene Polymorphisms and Somatic Cell Count in Milk of Jersey Cows. Acta Vet Brno 79: 237-242.
  • Liefers SC, Pas MF, Veerkamp RF, Van der Lende T (2002) Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. J Dairy Sci 85: 1633-1638. 
  • Liefers SC, Veerkamp RW, Te Pas MF, Delavaud C, Chilliard Y, Van der Lende T (2004) A missense mutation in the bovine leptin receptor gene is associated with leptin concentrations during late pregnancy. Anim Genet 35: 138-141. 
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  • Matteis G, Scatà MC, Grandoni F, Petrera F, Abeni F, Catillo G, Napolitano F, Moioli B (2012) Association analyses of single nucleotide polymorphisms in the leptin and leptin receptor genes on milk and morphological traits in Holstein cows. Open J Animal Science 2: 174-182.
  • Othman OE, Zayed FA, El Gawead AA (2011) Genetic polymorphism of two genes associated with carcass trait in Egyptian buffaloes. Genet Eng Biotechnol J 9: 15-20.
  • SAS Institute Inc. 2009. Administering SAS® Enterprise Guide® 4.2. Cary, NC: SAS Institute Inc.
  • Silva RC, Ferraz JB, Meirelles FV, Eler JP, Balieiro JC, Cucco DC, Mattos EC, Rezende FM, Silva SL (2012) Association of single nucleotide polymorphisms in the bovine leptin gene and leptin receptor genes with growth and ultrasound carcass traits in Nellore cattle. Genet Mol Res 11: (AOP). 
  • Wayne J, Kuenzel WJ, Fraley GS (1995) Neuropeptid Y: It´s in the Neural Regulation of Reproductive Function and Feed Intake in Mammalian Species. Poultry Avian Biol Rev 6: 185-209.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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