A novel polymorphism within intron B of growth hormone gene (GH2) of the rainbow trout, Oncorhynchus mykiss

• Autorzy: Panicz R. , Zych S. , Grzesiak W.

Warianty tytułu

PL

Charakterystka polimorfizmu w sekwencji intronu B genu hormonu wzrostu (GH2) pstrąga tęczowego, Oncorhynchus mykiss

• Języki publikacji: EN

Abstrakty

EN

Nucleotide composition of both growth hormone variants of rainbow trout (Oncorhynchus mykiss) has been strongly preserved evolutionally what might suggest that any change within these sequences can have an influence on the functioning of the somatotropic axis. A 121 bp fragment that contained nearly the entire B intron was amplified by the polymerase chain reaction. PCR products were bidirectionally sequenced. PCR products were digested by TaiI according to manufacturer’s instructions and resulting DNA was subjected to electrophoresis. An analysis of the gene fragment for growth hormone 2 showed the presence of SNP, easily identifiable by means of digestion with TaiI restriction enzyme. Statistical analysis confirmed that homozygous GHBB fish were the longest (31.77 cm) and the heaviest (404.70 g) and were statistically significantly different (P ≤ 0.05) from heterozygous GHAB fish. Mean length of GHAA homozygous fish was insignificantly lower (30.06 cm) with mean body weight of 339.12 g than homozygotes GHBB.

PL

Sekwencja nukleotydowa obu wariantów genu hormonu wzrostu pstrąga tęczowego (Oncorhynchus mykiss) jest silnie zakonserwowana ewolucyjnie. Każde nowo powstałe miejsce polimorficzne może mieć więc wpływ na funkcjonowanie osi somatotropowej. Badano niepełną sekwencję intronu B genu hormonu wzrostu typu 2 o długości 121 pz, uzyskaną po przeprowadzeniu dwukierunkowej reakcji sekwencjonowania. Otrzymane produkty PCR poddano analizie z wykorzystaniem endonukleazy TaiI, a wynik tej reakcji zobrazowano poprzez rozdział otrzymanych fragmentów restrykcyjnych w żelu agarozowym. Podczas analiz wykazano obecność mutacji punktowej, którą można łatwo zidentyfikować poprzez trawienie sekwencji nukleotydowej intronu B enzymem restrykcyjnym TaiI. W analizie statystycznej otrzymanych danych potwierdzono, że homozygotyczne GHBB pstrągi o średniej długości 31,77 cm oraz masie 404,70 g różniły się statystycznie istotnie (P ≤ 0,05) od osobników heterozygotycznych GHAB. Średnia długość (30,06 cm) oraz masa (339,12 g) homozygotycznych GHAA ryb były nieistotnie niższe od analogicznych parametrów osobników z genotypem GHBB. Scharakteryzowany polimorfizm ma istotny statystycznie wpływ na tempo wzrostu badanych osobników pstrąga tęczowego.

Słowa kluczowe

EN

polymorphism; growth hormone gene; rainbow trout; Oncorhynchus mykiss; PCR-RFLP method

• Wydawca: -
• Czasopismo: Polish Journal of Natural Sciences
• Rocznik: 2014
• Tom: 29
• Numer: 2
• Opis fizyczny: p.153-160,fig.,ref.

Twórcy

autor

Panicz R.

• Department of Aquaculture, West Pomeranian University of Technology in Szczecin, Kazimierza Krolewicza 4, 71-550 Szczecin, Poland

autor

Zych S.

• Department of Aquaculture, West Pomeranian University of Technology in Szczecin, Kazimierza Krolewicza 4, 71-550 Szczecin, Poland

autor

Grzesiak W.

• Department of Ruminants Science, West Pomeranian University of Technology in Szczecin, Szczecin, Poland

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