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2014 | 67 | 4 |
Tytuł artykułu

Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley

Treść / Zawartość
Warianty tytułu
PL
Wpływ cytokinin na aktywność enzymów antyoksydacyjnych u Pelargonium hortorum L. H. Bayley w warunkach in vitro
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to determine the influence of meta-topolin (mT) and 6-benzyl-aminopurine (BAP) on the hydrogen peroxide (H2O2) level and antioxidant enzymes activities in relation to the shoot formation and senescence process in Pelargonium hortorum cultivars, which differ in their susceptibility to leaf yellowing under in vitro conditions. In an early senescing cultivar ‘Grand Prix’, the addition of an aromatic cytokinin mT to abscisic acid (ABA)-enriched Murashige and Skoog (MS) basal medium more efficiently inhibited leaf yellowing than BAP. In both genotypes, meta-topolin was also the most effective in shoot formation. It was found that Pelargonium species varying in their susceptibility to senescence differ in H2O2 production and antioxidant enzymes activities. Generally, meta-topolin more effectively enhanced H2O2 production and POD activity than BAP and control medium, but its effect depended on genotype. The highest H2O2 production stimulated by mT was observed on day 5 of subculture in late senescing cv. ‘Bergpalais’. In both geranium genotypes, superoxide dismutase (SOD) and catalase (CAT) levels were highest at the beginning of the subculture period, during the initiation of shoot formation. SOD showed the highest activity on day 5 of subculture on the medium without cytokinin and generally being higher in cv. ‘Bergpalais’ than in cv. ‘Grand Prix’. CAT activity was positively regulated by both cytokinins. POD activity was most effectively enhanced by mT, but on different days of subculture - on the 2nd day of subculture in cv. ‘Bergpalais’ and on the 22nd day of subculture in cv. ‘Grand Prix’. The enhanced activity of POD in the presence of mT, 4-fold higher than on control medium, at the end of subculture in P. hortorum ‘Grand Prix’ coincided with the inhibition of leaf senescence.
PL
Badano wpływ cytokinin (mT i BAP) na tworzenie H2O2 i aktywność enzymów antyoksydacyjnych – dysmutazy ponadtlenkowej (SOD), katalazy (CAT) i peroksydaz (POD) w procesie tworzenia i starzenia pędów in vitro u odmiany pelargonii rabatowej wolno ( Bergpalais’) i szybko starzejącej się ( ‚ Grand Prix’). U obydwu odmian najwyższy współczynnik mnożenia pędów i ich jakość uzyskano w obecności mT. Przy łącznym podaniu z ABA, mT znacząco obniżała żółknięcie liści u odmiany ‚ Grand Prix’. Odmiany pelargonii różniły się zdolnością do akumulacji H2O2 i aktywnością enzymów antyoksydacyjnych, a ważnym czynnikiem wpływającym na ich poziom była cytokinina, głównie mT. W obecności meta-topoliny obserwowano najwyższą produkcję H2O2 w 5. dniu pasażu u odmiany Bergpalais’ i najwyższą aktywność POD u obydwu odmian, przy czym u odmiany ‚Bergpalais’ na początku pasażu a u odmiany ‚ Grand Prix’ w 22. dniu pasażu. U odmiany szybko starzejącej się, wzrost aktywności POD w obecności meta-topoliny, czterokrotnie wyższy w porównaniu z pożywką bez cytokininy był zbieżny z opóźnionym starzeniem pędów. U obydwu odmian, aktywność SOD i CAT była najwyższa na początku pasażu, w czasie indukcji tworzenia pędów. Najwyższy poziom SOD obserwowano na pożywce bez cytokininy, natomiast CAT pod wpływem cytokininy, zarówno mT jak i BAP. Sugeruje się, iż indukowana w obecności meta-topoliny produkcja H2O2 i aktywność POD mogą odgrywać istotną rolę w procesie tworzenia i starzenia pędów Pelargonium in vitro.
Słowa kluczowe
EN
Wydawca
-
Czasopismo
Rocznik
Tom
67
Numer
4
Opis fizyczny
p.33-41,fig.,ref.
Twórcy
autor
  • Research Institute of Horticulture, Skierniewice, Poland
autor
  • The F. Gorski Institute of Plant Physiology, Polish Academy of Sciences, Krakow, Poland
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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