In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

• Autorzy: Abdelkhalek A.E. , Gabr S.A. , Khalil W.A. , Shamiah S.H. , Pan L. , Qin G. , Farouk M.H.
• Języki publikacji: EN

Abstrakty

EN

Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in a blastocyst stage. This study represented a successful in vitro production of Sudanese dromedary camel embryos from epididymal sperm cells and in vitro matured oocytes recovered from slaughtered camels.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 1
• Opis fizyczny: p.95-101,fig.,ref.

Twórcy

autor

Abdelkhalek A.E.

• Animal Production Deptartement, Faculty of Agriculture, Mansoura University, Egypt

autor

Gabr S.A.

• Animal Production Department, Faculty of Agriculture Tanta University, Egypt

autor

Khalil W.A.

• Animal Production Deptartement, Faculty of Agriculture, Mansoura University, Egypt

autor

Shamiah S.H.

• Agriculture Research Center, Animal Production Research Institute, Giza, Egypt

autor

Pan L.

• College of Animal Science and Technology, Jilin Agricultural University, Changchun, China

autor

Qin G.

• College of Animal Science and Technology, Jilin Agricultural University, Changchun, China

autor

Farouk M.H.

• College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
• Animal Production Deptartment, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt

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Identyfikatory

DOI

10.1515/pjvs-2017-0013