Protein changes and proteolytic degradation in red and white clover plants subjected to waterlogging

• Autorzy: Stoychev V , Simova-Stoilova L. , Vaseva I. , Kostadinova A. , Nenkova R. , Feller U. , Demirevska K.
• Języki publikacji: EN

Abstrakty

EN

Red (Trifolium pratense L., cv. ‘‘Start’’) and white clover varieties (Trifolium repens L., cv. ‘‘Debut’’ and cv. ‘‘Haifa’’) were waterlogged for 14 days and subsequently recovered for the period of 21 days. Physiological and biochemical responses of the clover varieties were distinctive, which suggested different sensitivity toward flooding. The comparative study of morphological and biochemical parameters such as stem length, leaflet area, dry weight, protein content, protein pattern and proteolytic degradation revealed prominent changes under waterlogging conditions. Protease activity in the stressed plants increased significantly, especially in red clover cv. ‘‘Start’’, which exhibited eightfold higher azocaseinolytic activity compared to the control. Changes in the protein profiles were detected by SDS-PAGE electrophoresis. The specific response of some proteins (Rubisco, Rubisco-binding protein, Rubisco activase, ClpA and ClpP protease subunits) toward the applied stress was assessed by immunoblotting. The results characterized the red clover cultivar ‘‘Start’’ as the most sensitive toward waterlogging, expressing reduced levels of Rubisco large and small subunits, high content of ClpP protease subunits and increased activity of protease isoforms.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 06
• Opis fizyczny: p.1925-1932,fig.,ref.

Twórcy

autor

Stoychev V

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg 21, 1113 Sofia, Bulgaria

autor

Simova-Stoilova L.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg 21, 1113 Sofia, Bulgaria

autor

Vaseva I.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg 21, 1113 Sofia, Bulgaria

autor

Kostadinova A.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg 21, 1113 Sofia, Bulgaria

autor

Nenkova R.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg 21, 1113 Sofia, Bulgaria

autor

Feller U.

• Institute of Plant Sciences and Oeschger Centre for Climate Changes Research (OCCR), University of Bern, Altenbergrain 21, 3013 Bern, Switzerland

autor

Demirevska K.

• Plant Stress Molecular Biology Department, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg 21, 1113 Sofia, Bulgaria

Bibliografia

• Ahsan N, Lee DG, Lee SH, Kang KY, Bahk JD, Choi MS, Lee IJ, Renaut J, Lee BH (2007a) A comparative proteomic analysis of tomato leaves in response to waterlogging stress. Physiol Plant 131(4):555–570
• Ahsan N, Lee DG, Lee SH, Lee KW, Bahk JD, Lee BH (2007b) A proteomic screen and identification of waterlogging-regulated proteins in tomato roots. Plant Soil 295:37–51
• Aschi-Smiti S, Chaibi W, Brouquisse R, Ricard B, Saglio P (2003) Assessment of enzyme induction and aerenchyma formation as mechanisms for flooding tolerance in Trifolium subterraneum ‘Park’. Ann Bot 91(2):195–204
• Bailey-Serres J, Voesenek LACJ (2008) Flooding stress: acclimations and genetic diversity. Annu Rev Plant Biol 59:313–339
• Beers EP, Jones AM, Dickerman AW (2004) The S8 serine, C1A cysteine and A1 aspartic protease families in Arabidopsis. Phytochem 65:43–58
• Beha EM, Theodorou MK, Thomas BJ, Kingston-Smith AH (2002) Grass cells ingested by ruminants undergo autolysis which differs from senescence: implications for grass breeding targets and livestock production. Plant Cell Environ 25:1299–1312
• Beyene G, Foyer ChH, Kunert KJ (2006) Two new cysteine proteinases with specific expression patterns in mature and senescent tobacco (Nicotiana tabacum L.) leaves. J Exp Bot 57(6):1431–1443
• Bogeat-Triboulot MB, Brosche M, Renaut J, Jouve L, Le Thiec D, Fayyaz P, Vinocur B, Witters E, Laukens K, Teichmann T, Altman A, Hausman J-F, Polle A, Kangasjarvi J, Dreyer E (2007) Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology, and growth performance in Populus euphratica, a poplar growing in arid regions. Plant Physiol 143:876–892
• Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254
• Colmer TD, Voesenek LACJ (2009) Flooding tolerance: suites of plant traits in variable environments. Funct Plant Biol 36: 665–681
• Demirevska K, Simova-Stoilova L, Vassileva V, Feller U (2008a) Rubisco and some chaperone protein responses to water stress and rewatering at early seedling growth of drought sensitive and tolerant wheat varieties. Plant Growth Regul 56(2):97–106
• Demirevska K, Simova-Stoilova L, Vassileva V, Vaseva I, Grigorova B, Feller U (2008b) Drought induced leaf protein alterations in sensitive and tolerant wheat varieties. Gen Appl Plant Physiol 34(1–2):79–102
• Directive 2007/60/EC of the European parliament and of the council of 23 October 2007 on the assessment and management of flood risks. Off J Eur Union L: 27–34
• Feller U, Anders I, Demirevska K (2008) Degradation of Rubisco and other chloroplast proteins under abiotic stress. Gen Appl Plant Physiol 34(1–2):5–18
• Fisher A, Feller U (1993) The pattern of peptide hydrolase activities in shoots of field-grown winter wheat during the cold season. Agronomie 13:293–299
• Hashiguchi A, Ahsan N, Komatsu S (2010) Proteomics application of crops in the context of climatic changes. Food Res Int 43:1803–1813
• Irfan M, Hayat S, Hayat Q, Afroz S, Ahmad A (2010) Physiological and biochemical changes in plants under waterlogging. Protoplasma 241:3–17
• Jackson MB (2003) The impact of flooding stress on plants and crops. School of Biological Sciences, University of Bristol, Bristol, pp 1–15
• Kingston-Smith AH, Bollard AL, Minchin FR (2005) Stress-induced changes in protease composition are determined by nitrogen supply in non-nodulating white clover. J Exp Bot 56(412): 745–753
• Kokubun N, Ishida H, Makino A, Mae T (2002) The degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase into the 44-kDa fragment in the lysates of chloroplasts incubated in darkness. Plant Cell Physiol 43(11): 1390–1395
• Kosová K, Vıtámvás P, Prášil IT, Renaut J (2011) Plant proteome changes under abiotic stress—contribution of proteomics studies to understanding plant stress response. J Proteom 74:1301–1322
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680–685
• Mitsuhashi W, Feller U (1992) Effects of light and external solutes on the catabolism of nuclear-encoded stromal proteins in intact chloroplasts isolated from pea leaves. Plant Physiol 100: 2100–2105
• Nakano R, Ishida H, Kobayashi M, Makino A, Mae T (2010) Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions. Plant Biol 12:35–45
• Rijke E, Aardenburg L, van Dijk J, Ariese F, Ernst WHO, Gooijer C, Brinkman UATH (2005) Changed isoflavone levels in red clover (Trifolium pratense L.) leaves with disturbed root nodulation in response to waterlogging. J Chem Ecol 31(6): 1285–1298
• Salvucci ME, Portis AR, Ogren WL (1985) A soluble chloro-plast protein catalyses ribulosebisphosphate carboxylase/oxygenase activation in vivo. Photosynt Res 7:193–201
• Sanderson MA, Byers RA, Skinner RH, Elwinger GF (2003) Growth and complexity of white clover stolons in response to biotic and abiotic stress. Crop Sci 43:2197–2205
• Seker H, Rowe DE, Brink GE (2003) White clover morphology changes with stress treatments. Crop Sci 43:2218–2225
• Simova-Stoilova L, Demirevska K, Kingston-Smith A, Feller U (2012) Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Sci 183:43–49
• Sullivan ML, Hatfield RD (2006) Polyphenol oxidase and o-diphenols ihibit postharvest proteolysis in red clover and alfalfa. Crop Sci 46:662–670
• Van der Hoorn RAL (2008) Plant proteases: from phenotypes to molecular mechanisms. Annu Rev Plant Biol 59:191–223
• Vaseva I, Akiscan Y, Demirevska K, Anders I, Feller U (2011) Drought stress tolerance of red and white clover—comparative analysis of some chaperonins and dehydrins. Scient Horticult 130:653–659
• Vaseva I, Akiscan Y, Simova-Stoilova L, Kostadinova A, Nenkova R, Anders I, Feller U, Demirevska K (2012) Antioxidant response to drought in red and white clover. Acta Physiol Plant 34(5):1689–1699. doi:10.1007/s11738-012-0964-4
• Vassileva V, Demirevska K, Simova-Stoilova L, Petrova T, Tsenov N, Feller U (2011) Winter wheat cultivars under long-term field drought—biochemical and ultrastructural constraints affecting yield. J Agron Crop Sci 198(2):104–117. doi:10.1111/j.1439-037X.2011.00492.x
• Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response (Review). Trends Plant Sci 9(5):244–251

Uwagi

rekord w opracowaniu