Acid alpha-galactosidase is involved in D-chiro-inositol accumulation during tartary buckwheat germination

• Autorzy: Jia C.-F. , Hu W.-H. , Chang Z. , Gao H.-L.
• Języki publikacji: EN

Abstrakty

EN

Tartary buckwheat seed and especially its sprouts are rich in D-chiroinositol (DCI). The research was to evaluate when DCI was most accumulated in tartary buckwheat sprouts. In addition, we explored the activity and expression pattern of α-galactosidase during tartary buckwheat seed germination. The results showed that DCI contents steadily increased at early stage of germination and reached the highest level of 33.42 μg/seed at 24 h during the 72 h trail. However, the total fagopyritol contents sharply decreased from 214.6 μg/seed to 46 μg/seed at the end of the germination. The activity of acid α-galactosidase increased gradually to the peak of 0.36 nkat/seed at 24 h after the primed seed imbibition. We cloned the gene fragment of α-galactosidase in tartary buckwheat for the first time. The deduced amino acid sequence is 93% identical to that of P. vulgaris. The quantitative PCR result of gene expression pattern was consistent with its enzyme activity during seed germination.

Słowa kluczowe

EN

alpha-galactosidase; D-chiro-inositol; accumulation; tartary buckwheat; Fagopyrum tataricum; duckwheat zob.tartary buckwheat; India buckwheat zob.tartary buckwheat; India wheat zob.tartary buckwheat; green buckwheat zob.tartary buckwheat; germination; gene expression

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2015
• Tom: 84
• Numer: 1
• Opis fizyczny: p.53-58,fig.,ref.

Twórcy

autor

Jia C.-F.

• School of Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, China

autor

Hu W.-H.

• School of Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, China

autor

Chang Z.

• School of Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, China

autor

Gao H.-L.

• School of Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, China

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Identyfikatory

DOI

10.5586/asbp.2015.002