The effects of swimming exercise and supraphysiological doses of nandrolone decanoate on the testis in adult male rats: a transmission electron microscope study

• Autorzy: Naraghi M.A. , Abolhasani F. , Kashani I. , Anarkooli I. J. , Hemadi M. , Azami A. , Barbarestani M. , Aitken R. J. , Shokri S.
• Języki publikacji: EN

Abstrakty

EN

Anabolic-androgenic steroids (AAS) are used in high doses by athletes to improve athletic ability, physical appearance, and muscle mass. Unfortunately, the abuse of these agents has significantly increased. It has been established that exercise and high doses of AAS may influence the hypothalamic-pituitary gonadal (H-P-G) axis, which can in turn affect the ultrastructure of the testes. However, the effect of the combination of exercise and high doses of AAS on the ultrastructure of the testes is not known. This study was undertaken in order to examine the combination effects of swimming exercise and supraphysiological doses of nandrolone decanoate on the ultrastructural changes in rat testes. Five groups of male Wistar strain albino rats were treated as follows for 8 weeks: solvent of nandrolone decanoate (peanut oil) as a vehicle (sham); nandrolone decanoate (ND) (10 mg/kg/week) — ND; exercise (1 h/day, 5 days a week) — exercise; ND (10 mg/kg/week) and exercise (1 h/day, 5 days a week) — ND-EX; and sedentary control without any injection or exercise — control. Ultrastructural changes in the rat testes were characterised by transmission electron microscopy. The number and size of Leydig cells were considerably decreased in the interstitial space in the experimental rats. The increased thickness and irregular wavy multilaminar appearance of basement membrane in the treated animals, especially in the ND-EX group, are associated with well developed myoid cells. Cytoplasm vacuolisation, vesicular-like crista of the mitochondria, numerous lipid droplets, and lysosome and phagolysosome in Sertoli cells were significantly observed in the experimental groups. Several apoptotic germ cells were considerably observed in the experimental rats (p ≤ 0.05). Exercise training seems to increase the extent of ultrastructural changes caused by supraphysiological doses of ND in rats, which in turn may affect fertility. (Folia Morphol 2010; 69, 3: 138–146)

Słowa kluczowe

EN

exercise effect; swimming exercise; supraphysiological dose; nandrolone decanoate; testis; adult; male; rat; transmission electron microscope

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2010
• Tom: 69
• Numer: 3
• Opis fizyczny: p.138-146,fig.,ref.

Twórcy

autor

Naraghi M.A.

• Anatomy Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Abolhasani F.

• Anatomy Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Kashani I.

• Anatomy Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Anarkooli I. J.

• Anatomy Department, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

autor

Hemadi M.

• Anatomy Department, School of Medicine, Ahvaz University of Medical Sciences, Ahvaz, Iran

autor

Azami A.

• Anatomy Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Barbarestani M.

• Anatomy Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Aitken R. J.

• ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, University of Newcastle, NSW, Australia

autor

Shokri S.

• Anatomy Department, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

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