Immunohistochemical assessment of cell death in hepatocytes after anthracycline therapy

• Autorzy: Pedrycz A. , Boratynski Z. , Olesinski I. , Dudzinska M.
• Języki publikacji: EN

Abstrakty

EN

The study material consisted of 32 Wistar female rats The rats were divided into four equal groups. The rats from the experimental groups received adriamycin - 5 mg/kg b.w. to induce apoptosis and were decapitated after 4 and 7 weeks. After decapitation, specimens from the liver were collected, fixed in 10% formalin, and then embedded in paraffin blocks. Caspases 1, 3, 8, 9, and 12, Bcl-2, BAX, and Apaf-1 were detected using the standard three-step immunohistochemical method. Adriamycin-induced apoptosis in the hepatocytes occurred after 4 weeks and increased after 7 weeks (an increase for executing caspase). Irrespective of the time factor, it was induced mainly via the mitochondrial pathway (statistically-significant increase in reaction for BAX, Apaf-1, and caspase 9) and reticular pathway (statistically-significant increase in reaction for caspase 12). Apoptosis via the extrinsic pathway through caspase 8 activation was comparable to that in control (insignificant caspase 8 reaction). There was no increased reaction observed for caspase 1, one of the markers of inflammation.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.725-730,fig.,ref.

Twórcy

autor

Pedrycz A.

• Department of Histology and Embryology with Laboratory of Experimental Cytology, Medical University of Lublin, 20-080 Lublin, Poland

autor

Boratynski Z.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland

autor

Olesinski I.

• Department of Neurology I, Neuropsychiatric Hospital, 20-442 Lublin, Poland

autor

Dudzinska M.

• Department of Lungs Diseases and Tuberculosis, Jan Bozy Hospital Lublin, 20-089 Lublin, Poland

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