Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 59 | 2 |
Tytuł artykułu

Occurrence and characterisation of MRSA and extended-spectrum beta-lactamases producing Escherichia coli isolated from mastitic cows’ milk

Warianty tytułu
Języki publikacji
The aim of study was the preliminary evaluation of the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum ß-lactamases (ESBL) - producing Escherichia coli in 650 milk and inflammatory secretions from cows with clinical or subclinical mastitis. One millilitre of the sample was added to Mueller-Hinton broth supplemented with 6.5% NaCl, Tryptone Soya Broth with cefoxitin and aztreonam, and then to MRSA ID agar. Presumptive MRSA colonies were analysed for the presence of mecA gene. Parallel to MRSA identification, the samples were incubated in buffered peptone water, lauryl tryptose broth and McConkey agar supplemented with cefotaxim for ESBL-producing E. coli isolation. These bacteria were identified using API Rapid 32 E and the ability of ESBL production was initially established using disc test D68C and confirmed by MIC technique using Sensititre ESBL plates. The primers (blaCTX, blaTEM, blaSHV, and blaCMY-2-group) for the detection of some of the genes encoding ESBL production were used. The 45 strains of S. aureus with mecA gene and 41 strains of E. coli with blaTEM gene were detected.
Słowa kluczowe
Opis fizyczny
  • Department of Microbiology, 24-100 Pulawy, Poland
  • Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Department of Microbiology, 24-100 Pulawy, Poland
  • Department of Microbiology, 24-100 Pulawy, Poland
  • Department of Microbiology, 24-100 Pulawy, Poland
  • Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • 1. Abdel-Rady A., Saved M.: Epidemiological studies on subclinical mastitis in dairy cows in Assiut Governorate. Vet World 2009, 2, 373-380.
  • 2. Alekish M.O., Al.-Quadah K.M., Al.-Saleh A.: Prevalence of antimicrobial resistance among bacterial pathogens isolated from bovine mastitis in northern Jordan. Revue Méd Vét 2013, 164, 319-326.
  • 3. Bardiau M., Yamazaki K., Duprez J.N., Taminiau B., Mainil J.G., Ote I.: Genotypie and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolated from milk of bovine mastitis. Lett Appl Microbiol 2013, 57, 181-186.
  • 4. Bengtsson B., Ericsson Unnerstad H., Ekman T., Artusson K., Nilsson-Öst M., Persson Waller K.: Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol 2009, 136, 142-149.
  • 5. Beuron D.C., Cortinhas C.S., Botaro B.G., Macedo S.N., Gonçalves J.L., Brito M.A.V.P., Santos M.V.: Risk factors associated with the antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis. Pesq Vet Bras 2014, 34, 947-952.
  • 6. Carattoli A.: Animal reservoirs for extended spectrum ß-lactamase producers. Clin Microbiol Infect 2008, 14 (Suppl. 1), 117-123.
  • 7. Chandrasekaran D., Venkatesan P., Tirumurugaan K.G., Nambi A.P., Thirunavukkarasu P.S., Kumanan K., Vairamuthu S., Ramesh S.: Pattern of antibiotic resistant mastitis in dairy cows. Vet World 2014, 7, 389-394 doi: 10.14202/vetworld.2014.389-394.
  • 8. Dahmen S., Métayer V., Gay E., Madec J.Y., Haenni M.: Characterization of extended-spectrum beta-lactamase (ESBL)- carrying plasmids and clones of Enterobacteriaceae causing cattle mastitis in France. Vet Microbiol 2013, 162, 793-799.
  • 9. Ericsson Unnerstad H., Bengtsson B., Horn M., Börjesson S.: Methicillin-resistant Staphylococcus aureus containing mecC in Swedish dairy cows. Acta Vet Scand 2013, 55:6 doi: 10.1186/1751-0147-55-6.
  • 10. Ganguly S.: Mastitis, an economically significant bacterial disease of dairy cattle having immense public health importance: a comprehensive & exclusive review. Int J Pure App Biosci 2014, 2, 82-85.
  • 11. Geser N., Stephan R., Hächler H.: Occurrence and characteristics of extended-spectrum ß-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Vet Res 2012, 8, 21 doi:10.1186/1746-6148- 8-21.
  • 12. Gindonis V., Taponen S., Myllyniemi A.L., Nykasenoja S., Salmenlinna S., Lindholm L., Rantala M.: Occurrence and characterisation of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland. Acta Vet Scand 2013, 55, 61.
  • 13. Halasa T., Huijps K., Osterås O., Hogeveen H.: Economic effects of bovine mastitis and mastitis management: A review. Vet Quarterly 29:1, 18-31 doi: 10.1080/01652176.2007. 9695224.
  • 14. Jossi L.R., Devkota S.P.: Methicillin resistant Staphylococcus aureus (MRSA) in cattle: epidemiology and zoonotic implications. Int J Appl Sci Biotechnol 2:29-33. doi: 10.3126/ijasbt.v2i1.9861.
  • 15. Juhász-Kaszanyitzki E., Jánosi S., Somogyi P., Dán A., van der Graaf-van Blois L., van Dujkaren E., Wagenaar J.A.: MRSA Transmission between cows and humans. Emerg Infect Dis 2007, 13,630-632.
  • 16. Kalmus P.: Clinical mastitis in Estonia: diagnosis, treatment efficacy and antimicrobial resistance of pathogens in Estonia. Thesis, Estonian University of Life Sciences, 2013.
  • 17. Kurjogi M.M., Kaliwal B.B.: Prevalence and antimicrobial susceptibility of bacteria isolated from bovine mastitis. Adv Appl Sci Res 2011, 2, 229-235.
  • 18. Malinowski E., Lassa H., Tomaszewska J., Malkińska- Horodyńska M.: Phenotypical identification of atypical Staphylococcus aureus strains isolated from milk of cows from one herd. Bull Vet Inst Pulawy 2011, 55, 211-215.
  • 19. Modi C.M., Patel H.B., Patel H.B., Mody S.K.: A comprehensive review on pharmacotherapeutics of bovine mastitis. Mol Microbiol Res 2012, 2, 1-9.
  • 20. Ohnishi M., Okatani A.T., Harada K., Sawada T., Marumo K., Murakami M., Sato R., Esaki H., Shimura K., Kato H., Uchida N., Takahashi T.: Genetic characteristics of CTX-M- type extended-spectrum-ß-lactamase (ESBL-)-producing Enterobacteriaceae involved in mastitis cases on Japanese dairy farms, 2007 to 2011. J Clin Microbiol 2013, 51, 3117-3122.
  • 21. Oliver S.P., Jayarao B.M., Almeida R.A.: Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Path Dis 2005, 2, 115-124.
  • 22. Paul I., Ganguly S.: Bovine mastitis, an economically important bacterial infection of udder in cattle: a review. Ind J Sci Res Tech 2014, 2, 1-2.
  • 23. Saini V., McClure J.T., Léger D., Keefe G.P., Scholl D.T., Morek D.W., Barkema H.W.: Antimicrobial resistance proflies of common mastitis pathogens on Canadian dairy farms. J Dairy Sci 2012, 95, 4319-4332.
  • 24. Su Y., Yu C-Y., Tsai Y., Wang S-H, Lee C., Chu C: Fluoroquinolone resistant and extended spectrum β-lactamase (ESBL)-producing Escherichia coli from milks of cow with clinic mastitis in northern Taiwan. J Microbiol Immunol Infect 2014 doi: 10.1016/j.jmii.2014.10.003.
  • 25. Tavakol M., Olde Riekerink R.G.M., Sampimon O.C., van Wamel W.J.B., van Belkum A., Lam T.J.G.M.: Bovine- associated MRSA ST398 in the Netherlands. Acta Vet Scand 2012, 54:28 doi: 10.1186/1751-0147-54-28.
  • 26. Timofte D., Maciuca I.E., Evans N.J., Williams H., Wattret A., Fick J.C., Williams N.J.: Detection and molecular characterisation of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 producing β-lactamases isolated from cases of bovine mastitis in the United Kingdom. Antimicrob Agents Chemother 2013 doi: 10.1128/AAC.00752-13.
  • 27. Turkyilmaz S., Tekbiyik S., Oryasin E., Bozdogan B.: Molecular epidemiology and antimicrobial resistance mechanism of methicillin-resistant Staphylococcus aureus isolated from bovine milk. Zoonoses Public Hlth 2010, 57, 197-203.
  • 28. Wang D., Wang Z., Yan Z., Wu J., Ali T., Li J., Lv Y., Han B.: Bovine mastitis Staphylococcus aureus: Antibiotic susceptibility profile, resistant genes and molecular typing of methicillin- resistant and methicillin-sensitive strains in China. Infect Genet Evol 2015, 31, 9-16.
  • 29. Wasiński B., Różańska H., Osek J.: Occurrence of extended spectrum ß-lactamase and AmpC-producing Escherichia coli in meat samples. Bull Vet Inst Pulawy 2013, 57, 513-517.
  • 30. Watson E., Jeckel S., Snow L., Stubbs R., Teale Ch., Wearing H., Horton R., Toszeghv M., Tearne O., Ellis- Iversen J., Coldham N.: Epidemiology of extended spectrum beta-lactamase E. coli (CTX-M-15) on a commercial dairy farm. Vet Microbiol 2012, 154, 339-346.
  • 31. Zadoks R.N., Fitzpatrick J.L.: Changing trends in mastitis. Irish Vet J 2009, 62, Suppl, 59-70.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.