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2012 | 56 | 4 |

Tytuł artykułu

Cytotoxicity of some nitroimidazole derivatives - comparative studies on human and rat hepatoma cell lines

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The cytototoxic potential of metronidazole, tinidazole, ronidazole, and ornidazole, using human and rat hepatoma cell lines (HepG2 and FaO) in culture was assessed. The cells were treated with drugs for 24, 48 and 72 h at 37 °C in 5% CO₂ at concentrations of 0.1 to 200 µg/mL. Following the treatment period, the cells were assayed by four independent assays: MTT reduction, neutral red uptake (NRU), total protein content (TPC), and LDH leakage. The results suggest that nitroimidazoles are of low cytotoxic potential (EC₅₀ >200µg/mL). The exception was ronidazole, which demonstrated a distinct endpoint sensitivity related to the species. EC₅₀ (µg/mL) in human cells were: in MTT assay - 196±5.5 and 122±9.3 at 24 and 48 h, respectively, and in NRU assay - 150±1.25 at 72 h. Based on minimal toxic concentrations (EC₂₀) for ronidazole, determined by all methods used in HepG2 cells, it could be concluded that their sensitivity was as follows: MTT>NRU>LDH>TPC.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

56

Numer

4

Opis fizyczny

p.579-584,fig.,ref.

Twórcy

autor
  • Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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  • 14. Pohjala L., Tammela P., Samanta S.K., Yli-Kauhaluoma J., Vuorela P.: Assessing the data quality in predictive toxicology using a panel of cell lines and cytotoxicity assays. Anal Biochem 2007, 362, 221-228.
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Bibliografia

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