Expression of caspase-1 and caspase-3 mRNA in the developing and regressing ovary of the chicken embryo

• Autorzy: Grzegorzewska A. K.
• Języki publikacji: EN

Abstrakty

EN

In the chicken female embryo the gonads develop asymmetrically. Only the left ovary becomes a functional gonad and the right one regresses. The aim of this study was to evaluate the participation of apoptosis in the processes of development and regression by detection of mRNA expression of initiator caspase-1 and executive caspase-3 in the left and right ovaries at days 12 (E12) and 15 (E15) of embryogenesis. Total RNA from the ovaries was isolated and the mRNA expression of the analyzed genes was detected by using the RT-PCR method. The expression of caspase-1 and caspase-3 was observed in the left and right ovaries of the chicken embryo at days 12 and 15 of embryogenesis. In the left ovary the expression of the two analyzed genes did not differ between the two analyzed stages. In the right ovary the expression of caspase-1 mRNA significantly decreased on the 15th day of incubation in comparison with the 12th day, whereas the expression of caspase-3 significantly increased on the 15th day. The observed changes in mRNA expression of caspases suggest that initiator caspase-1 is expressed in the early phase of ovary regression, while caspase-3 is expressed in the later stage, which affects the execution of apoptosis.

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Zoologia
• Rocznik: 2012
• Tom: 54
• Opis fizyczny: p.53-58,fig.,ref.

Twórcy

autor

Grzegorzewska A. K.

• Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al.Mickiewicza 24/28, 30-059 krackw, Poland

Uwagi

PL

Rekord w opracowaniu