Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

• Autorzy: Niedzwiedz A. , Jaworski Z. , Kubiak K.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the levels of circulating immune complexes (CICs) and concentration of haptoglobin, fibrinogen and C-reactive protein in the serum of horses with recurrent airway obstruction and healthy controls. The study was conducted on a group of 14 adult Polish Konik horses, kept in uniform environmental and living conditions. Horses were divided into two groups: 7 horses were not affected by any respiratory problem (control group) and 7 horses had a history of recurrent airway obstruction (RAO) (study group). A clinical and laboratory evaluation, endoscopic examination and bronchoalveolar lavage (BAL) were performed in all horses. Levels of circulating immune complexes were significantly (p=0.0057) increased in heaves-affected horses compared to healthy controls (median [25th - 75th percentiles]) (3.96 [3.96 - 4.43] vs. 7.46 [5.13 - 11.9]). No significant difference was observed in the levels of the examined acute phase proteins between the groups. Moreover, all results were within the reference range established for horses. The results of this study indicate that heaves in horses is associated with the formation and high level of CICs. Haptoglobin, fibrinogen and C-reactive protein failed as markers of early stage systemic inflammation in the course of RAO.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 4
• Opis fizyczny: p.697-702,fig.,ref.

Twórcy

autor

Niedzwiedz A.

• Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

autor

Jaworski Z.

• Department of Horse Breeding and Riding, University of Warmia and Mazury in Olsztyn, Prawochenskiego 2, 10-720 Olsztyn, Poland

autor

Kubiak K.

• Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

Bibliografia

• Cray C (2012) Acute phase proteins in animals. Prog Mol Biol Transl Sci 105: 113-150.
• Cywińska A, Szarska E, Górecka R, Witkowski L, Hecold M, Bereznowski A, Schollenberger A, Winnicka A (2012) Acute phase protein concentrations after limited distance and long distance endurance rides in horses. Res Vet Sci 93: 1402-1406.
• Fernandez NJ, Hecker KG, Gilroy CV, Warren AL, Léguillette R (2013) Reliability of 400-cell and 5-field leukocyte differential counts for equine bronchoalveolar lavage fluid. Vet Clin Pathol 42: 92-98.
• Hašková V, Kašlik J, Riha I, Matl I, Rovenský J (1978) Simple method of circulating immune complex detection in human sera by polyethylene glycol precipitation. Z Immunitatsforsch Immunobiol. 154: 399-406.
• Heyman B (1990) The immune complex: possible ways of regulating the antibody response. Immunol Today 11: 310-313.
• Jones GE, Mould DL (1984) Adaptation of the guaiacol (peroxidase) test for haptoglobins to a microtitration plate system. Res Vet Sci 37: 87-92.
• Lavoie-Lamoureux A, Leclere M, Lemos K, Wagner B, Lavoie JP (2012) Markers of systemic inflammation in horses with heaves. J Vet Intern Med 26: 1419-1426.
• Leclere M, Lavoie-Lamoureux A, Lavoie JP (2011) Heaves, an asthma-like disease of horses. Respirology 16: 1027- -1046.
• Léguillette R (2003) Recurrent airway obstruction--heaves. Vet Clin North Am Equine Pract 19: 63-86.
• Maheswari H, Eswaran MA, Srividhya S, Malavika R, Prabhu R, Geetha KR (2014) Estimation of Circulating Immune Complexes in patients with Oral Leukoplakia and Oral Submucous Fibrosis: A Case Control Study. J Clin Diagn Res 8: 224-227
• Murunikkara V, Rasool M (2014) Trikatu, an herbal compound as immunomodulatory and anti-inflammatory agent in the treatment of rheumatoid arthritis-An experimental study. Cell Immunol 287: 62-68.
• Navolotskaya EV (2014) The second life of antibodies. Biochemistry (Mosc) 79: 1-7.
• Niedzwiedz A, Jaworski Z, Tykalowski B, Smialek M (2014) Neutrophil and macrophage apoptosis in bronchoalveolar lavage fluid from healthy horses and horses with recurrent airway obstruction (RAO). BMC Vet Res 10: 29.
• Nowak-Łoś L, Odrowaz-Sypniewska G, Zegarska J, Zalewska-Zacharek M, Kłyszejko-Molska J, Grabiec M, Adamczak R (2013) Analysis of serum protein fractions from women with recurrent miscarriage. Ginekol Pol 84: 851-856.
• Pandey MK (2013) Molecular basis for downregulation of C5a-mediated inflammation by IgG1 immune complexes in allergy and asthma. Curr Allergy Asthma Rep 13: 596-606.
• Pollock PJ, Prendergast M, Schumacher J, Bellenger CR (2005) Effects of surgery on the acute phase response in clinically normal and diseased horses. Vet Rec 156: 538-542.
• Riihimäki M, Raine A, Elfman L, Pringle J (2008) Markers of respiratory inflammation in horses in relation to seasonal changes in air quality in a conventional racing stable. Can J Vet Res 72: 432-439.
• Samuel AM, Ashtekar MD, Ganatra RD (1984) Significance of circulating immune complexes in pulmonary tuberculosis.Clin Exp Immunol 58: 317-324.
• Wener MH (2014) Tests for circulating immune complexes. Methods Mol Biol 1134: 47-57.

Identyfikatory

DOI

10.2478/pjvs-2014-0101