The effect of T-2 toxin on percentages of CD4plus, CD8plus, CD4plusCD8plus and CD21plus lymphocytes, and mRNA expression levels of selected cytokines in porcine ileal Peyer’s patches

• Autorzy: Obremski K. , Podlasz P. , Zmigrodzka M. , Winnicka A. , Wozny M. , Brzuzan P. , Jakimiuk E. , Wojtacha P. , Gajecka M. , Zielonka L. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

The immune system is one of the main toxicity targets of the T-2 toxin. In view of scant research data demonstrating the effect of T-2 on cellular and humoral responses in gut-associated lymphoid tissue (GALT), this study set out to investigate the effects of chronic exposure to low doses of the T-2 toxin (200 μg T-2 toxin kg⁻¹ feed) on percentages of CD4⁺ and CD8⁺ T lymphocytes, CD4⁺/CD8⁺ double-positive T lymphocytes, CD21⁺ B cells, and IL-2, IFN-γ, IL-4 and IL-10 mRNA expression levels in porcine ileal Peyer’s patches. The investigated material comprised ileum sections sampled from piglets (aged 8-10 weeks, body weight of 15-18 kg) on days 14, 28 and 42 of the experiment. After 42 days of exposure to T-2, a significant drop in the quantity of the IL-10 product was observed (R=0.94; S.E. 0.49-0.79; p<0.001). A gradual decrease in the amount of IL-4 and IFN-γ cytokine transcripts was found throughout the experiment, but the reported trend was not significant. On experimental days 14 and 42, a significant increase in the percentage of CD8⁺ T lymphocytes was observed in comparison with the control (p=0.04 and p=0.05, respectively), whereas on day 28, a significant decrease in the percentage of the above subpopulation was noted (p=0.00). The percentage of CD21⁺ B cells in the experimental group decreased steadily in comparison with the control, and the observed drop was significant on days 28 and 42 (p=0.06 and p=0.00, respectively). On days 14 and 28, the percentages of CD4⁺ and CD8⁺ T lymphocytes were lower in the experimental animals than in the control group, and the drop reported on day 28 was statistically significant (p=0.03).

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 2
• Opis fizyczny: p.341-349,fig.,ref.

Twórcy

autor

Obremski K.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Podlasz P.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Zmigrodzka M.

• Department of Pathology and Veterinary Diagnostics, Division of Animal Pathophysiology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland

autor

Winnicka A.

• Department of Pathology and Veterinary Diagnostics, Division of Animal Pathophysiology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland

autor

Wozny M.

• Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Słoneczna 45g, 10-709 Olsztyn, Poland

autor

Brzuzan P.

• Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Słoneczna 45g, 10-709 Olsztyn, Poland

autor

Jakimiuk E.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Wojtacha P.

• Department of Immunology, Genetics and Microbiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Jagielońska 78, 10-357 Olsztyn, Poland

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Zielonka L.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Gajecki M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

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