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2014 | 58 | 1 |
Tytuł artykułu

Changes in the expression of galanin and galanin receptors in the wall of the colon in pigs experimentally infected with Brachyspira hyodysenteriae

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The expression of galanin (GAL) and its three receptors (GalR1, GalR2, and GalR3) were studied with real-time PCR in the colonic wall of pigs suffering from experimental colitis caused by the infection with Brachyspira hyodysenteriae. The expression was studied in the muscular membrane, mucosa/submucosa layer, and in lymphocytes isolated from mucosa/submucosa. The expression levels were normalized to glyceraldehyde-6-phosphate dehydrogenase (GAPDH) expression and compared to expression levels in control animals. GAL expression was found in all three studied compartments of the colonic wall. A significant decrease in GAL expression level was found in the mucosa/submucosa and in isolated lymphocytes, whereas the decrease was much less profound in the muscular membrane. In the case of galanin receptors their expression was found in all studied compartments of the colonic wall, however at different levels, as compared to GAPDH expression. The decrease of galanin receptors expression was found in all studied compartments of the colonic wall of the sick animals.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
58
Numer
1
Opis fizyczny
p.23-28,fig.,ref.
Twórcy
autor
  • Department of Pathophysiology, Forensic Veterinary Medicine and Administration
autor
  • Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
  • Department of Pathophysiology, Forensic Veterinary Medicine and Administration
autor
  • Department of Pathophysiology, Forensic Veterinary Medicine and Administration
autor
  • Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
autor
  • Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
autor
  • Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
autor
  • Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
Bibliografia
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  • 3. Benya R.V., Matkowskyj K.A., Danilkovich A., Hecht G.: Galanin causes CI- secretion in the human colon. Potential significance of inflammation-associated NF-kappa B activation on galanin-1 receptor expression and function. Ann N Y Acad Sci 1998, 863, 64-77.
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  • 12. Loftus E.V. Jr., Schoenfeld P., Sandborn W.J.: The epidemiology and natural history of Crohn's disease in population-based patient cohorts from North America: a systematic review. Aliment Pharmacol Ther 2002, 16, 51-60.
  • 13. Marrero J.A., Matkowskyj K.A., Yung K., Hecht G., Benya R.V.: Dextran sulfate sodium-induced murine colitis activates NF- κB and increases galanin-1 receptor expression. Am J Physiol Gastrointest Liver Physiol 2000, 278, 797 804.
  • 14. Matkowskyj K.A., Danilkovich A., Marrero J.. Savkovic S.D., Hecht G., Benya R.V.: Galanin-1 receptor up-regulation mediates the excess colonic fluid production caused by infection with enteric pathogens. Nat Med 2000, 6, 1048-1051.
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  • 20. Sternini C., Anselmi L., Guerrini S., Cervio E., Pham T., Balestra B., Vicini R., Baiardi P., D'agostino G.L., Tonini M.: Role of galanin receptor 1 in peristaltic activity in the guinea pig ileum. Neuroscience 2004, 125, 103-112.
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  • 22. Wang S., Ghibaudi L., Hashemi T., He C., Strader C., Bayne M., Davis H., Hwa J.J.: The GalR2 galanin receptor mediates galanin-induced jejunal contraction, but not feeding behavior, in the rat: differentiation of central and peripheral effects of receptor subtype activation. FEBS Lett 1998, 434, 277-282.
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-65a5ec9a-5a14-4038-86a8-d133332c38c7
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