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2017 | 86 | 3 |

Tytuł artykułu

Critical factors besides treatment dose and duration need to be controlled in Pb toxicity tests in plant cell suspension cultures

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The study was designed to determine the proper conditions for suspension culture of Viola tricolor cells in toxicity studies of Pb at different concentrations (0, 200, 500, 1000, 2000 μM) and exposure times (24, 48, 72 h). By forming insoluble salts with ions from the medium, lead (II) nitrate added to the medium decreased the initial 5.7–5.8 pH of the medium, depending on the Pb salt concentration and light intensity. In alamarBlue assays, we found no dose- or time-dependent effect of Pb on cell viability when we did not adjust pH and did not standardize the illumination conditions to correct the effect of lead-salt-induced turbidity. When effective illumination was adjusted to correct for turbidity at the highest lead concentration and pH was adjusted to 5.7–5.8, cell viability decreased with the increase of Pb(NO₃)₂ concentration and with treatment time. These experiments demonstrate that the toxic action of lead on cells in suspension depends strongly on culture conditions, and not only on the metal concentration and duration of treatment.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

86

Numer

3

Opis fizyczny

Article 3555 [5p.],fig.,ref.

Twórcy

autor
  • Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland
  • Department of Inorganic Chemistry, Jagiellonian University, Ingardena 3, 30-060 Cracow, Poland
autor
  • Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland
  • Department of Physiology and Toxicology of Reproduction, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland
autor
  • Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Cracow, Poland

Bibliografia

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  • 2. Bolan N, Kunhikrishnan A, Thangarajan R, Kumpiene J, Park J, Makino T, et al. Remediation of heavy metal(loid)s contaminated soils; to mobilize or immobilize? J Hazard Mater. 2014;266:141–166. https://doi.org/10.1016/j.jhazmat.2013.12.018
  • 3. Porrut B, Shahid M, Dumat C, Winterton P, Pinelli E. Lead uptake, toxicity, and detoxification in plants. Rev Environ Contam Toxicol. 2011;213:113–136. https://doi.org/10.1007/978-1-4419-9860-6_4
  • 4. Hettiarachchi GM, Pierzynski GM. Soil lead bioavailability and in situ remediation of lead-contaminated soils: a review. Environ Prog Sustain Energy. 2004;23(1):78–93. https://doi.org/10.1002/ep.10004
  • 5. Harter RD. Effect of soil pH on adsorption of lead, copper, zinc, and nickel. Soil Sci Soc Am J. 1983;47:47–51. http://doi.org/10.2136/sssaj1983.03615995004700010009x
  • 6. Norvell WA. Comparison of chelating agents as extractants for metals in diverse soil materials. Soil Sci Soc Am J. 1984;48:1285–1292. https://doi.org/10.2136/sssaj1984.03615995004800060017x
  • 7. Bernabé-Antonio A, lvarez LA, Buendýa-González L, Maldonado-Magaňa A, Cruz-Sosa F. Accumulation and tolerance of Cr and Pb using a cell suspension culture system of Jatropha curcas. Plant Cell Tissue Organ Cult. 2015;120:221–228. https://doi.org/10.1007/s11240-014-0597-y
  • 8. Lizhong Z, Cullen WR. Effects of some heavy metals on cell suspension cultures of Catharanthus roseus. Journal of Environmental Sciences. 1995;7(1):60–65
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  • 10. Poborilova Z, Opatrilova R, Babula P. Toxicity of aluminium oxide nanoparticles demonstrated using a BY-2 plant cell suspension culture model. Environ Exp Bot. 2013;91:1–11. https://doi.org/10.1016/j.envexpbot.2013.03.002
  • 11. Ding M, Wang X, Li Y. Acquired tolerance to cadmium following long-term acclimation to CdCl₂ in rice suspension cultures. Plant Cell Tissue Organ Cult. 2016;124:47–55. https://doi.org/10.1007/s11240-015-0873-5
  • 12. Clever HL, Johnston FJ. The solubility of some sparingly soluble lead salts: an evaluation of the solubility in water and aqueous electrolyte solution. J Phys Chem Ref Data. 1980;9:3. https://doi.org/10.1063/1.555628
  • 13. Słomka A, Libik-Konieczny M, Kuta E, Miszalski Z. Metallifierous and non-metalliferous populations of Viola tricolor represent similar mode of antioxidative response. J Plant Physiol. 2008;165:1610–1619. https://doi.org/10.1016/j.jplph.2007.11.004
  • 14. Słomka A, Kuta E, Szarek-Łukaszewska G, Godzik B, Kapusta P, Tylko G, et al. Violets of the section Melanium, their colonization by arbuscular mycorrhizal fungi and their occurrence on heavy metal heaps. J Plant Physiol. 2011;168:1191–1199. https://doi.org/10.1016/j.jplph.2011.01.033
  • 15. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962;15:473–479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • 16. Zhang YK, Zhu DF, Zhang YP, Chen HZ, Xiang J, Lin XQ. Low pH-induced changes of antioxidant enzyme and ATPase activities in the roots of rice (Oryza sativa L.) seedlings. PLoS One. 2015;10(2):e0116971. https://doi.org/10.1371/journal.pone.0116971
  • 17. Soares EV, Duarte APRS, Soares HMVM. Study of the suitability of 2-(N-morpholino) ethanesulfonic acid pH buffer for heavy metals accumulation studies using Saccharomyces cerevisiae. Chemical Speciation and Bioavailability. 2000;12(2):59–65. https://doi.org/10.3184/095422900782775535
  • 18. Salonikidis PS, Kirichenko SN, Tatjanenko LV, Schwarz W, Vasilets LA. Extracellular pH modulates kinetics of the Na⁺, K⁺-ATPase. Biochim Biophys Acta. 2000;1509:496–504. https://doi.org/10.1016/S0005-2736(00)00356-4

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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