Effect of LeERF1 and LeERF2 overexpression in the response to salinity of young tomato (Solanumlycopersicum cv. Micro-Tom) seedlings

• Autorzy: Hu N. , Tang N. , Yan F. , Bouzayen M. , Li Z.
• Języki publikacji: EN

Abstrakty

EN

Ethylene responsive factors (ERFs) are important transcriptional regulators involved in plant responses to abiotic stress. LeERF1 and LeERF2, two members of the ERF family in tomato (Solanum lycopersicum), have previously been cloned. In this study, we investigated the saltstress tolerance of transgenic tomato overexpressing LeERF1 and LeERF2. The transgenic lines had longer roots than wild-type (WT) plants under salt stress conditions. Furthermore, we examined physiological and biochemical indexes in the plants and found that overexpression of LeERF1 and LeERF2 enhanced the release of chlorophyll and free proline, but decreased the malondialdehyde contents of the plants. Transgenic tomato displayed higher superoxide dismutase and guaiacol peroxidase activity than WT tomato under high salinity conditions. Moreover, quantitative RT-PCR analysis revealed that the expression levels of salt stress-related genes, including TAS14, HVA22, LHA1, PR5, and RBOHC, which were upregulated in the transgenic plants. Therefore, overexpression of LeERF1 and LeERF2 positively modulates the ethylene-mediated response to salt stress in tomato.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1703-1712,fig.,ref.

Twórcy

autor

Hu N.

• Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Genetic Engineering Research Center, School of Life Sciences, Chongqing UNiversity, 400030 Chongqing, China

autor

Tang N.

• Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Genetic Engineering Research Center, School of Life Sciences, Chongqing UNiversity, 400030 Chongqing, China

autor

Yan F.

• Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Genetic Engineering Research Center, School of Life Sciences, Chongqing UNiversity, 400030 Chongqing, China

autor

Bouzayen M.

• INP-ENSA Toulouse, Genomique et Biotechnologie des Fruits, University de Toulouse, Avenue de l'Agrobiopole, BP 32607, 31326 Castanet-Tolosan, France
• INRA, UMR990 Genomique et Biotechnologie des Fruits, Chemin de Borde Rouge, 31326 Castanet-Tolosan, France

autor

Li Z.

• Key Laboratory of Functional Gene and New Regulation Technologies Under Chongqing Municipal Education Commission, Genetic Engineering Research Center, School of Life Sciences, Chongqing UNiversity, 400030 Chongqing, China

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Identyfikatory

DOI

10.1007/s11738-014-1545-5