In vitro antibacterial activity of several plant extracts and essential oils against Brucella melitensis

• Autorzy: Safi M. , Al-Mariri A.

Warianty tytułu

PL

Aktywność antybakteryjna wybranych wyciągów roślinnych i olejków eterycznych przeciwko Brucella melitensis

• Języki publikacji: EN

Abstrakty

EN

Medicinal plants are considered to be new resources for the production of agents that could act as alternatives to antibiotics in the treatment of antibiotic-resistant bacteria. The aim of this study was to evaluate the efficacy of some plants native to Syria in the treatment of brucellosis. In vitro activities of some essential oils and plant extracts of some medicinal plants against 89 Brucella melitensis isolates was determined by disc diffusion method at a concentration of 5%. The microdilution assay in the fluid medium was used to determine the MICs of essential oils and plant extracts. Among the evaluated herbs, only Thymus syriacus and Cinnamomum zeylanicum essential oils and Laurus nobilis plant extract showed a high activity against B. melitensis strains. Thus, minimal inhibitory concentration (MIC50) values for T. syriacus, C. zeylanicum, and L. nobilis against B. melitensis were 6.25, 3.125 and 6.25 μl/ml, respectively. Among studied essential oils and plant extracts, T. syriacus and C. zeylanicum essential oils, and L. nobilis plant extract were the most effective ones. Moreover, T. syriacus - C. zeylanicum combination was more effective than use of each of them alone. Then, T. syriacus and C. zeylanicum essential oils and L. nobilis plant extract could act as bactericidal agents against B. melitensis.

PL

Rośliny lecznicze są uważane za nowe surowce do produkcji związków, które mogą być używane jako alternatywa dla antybiotyków w leczeniu chorób wywołanych przez bakterie oporne na antybiotyki. Celem niniejszej pracy było określenie skuteczności niektórych roślin rosnących w Syrii w leczeniu brucelozy. Aktywność olejków eterycznych i wyciągów przeciw 89 szczepom Brucella melitensis badano metodą krążków bibułowych, stosując 5-cio% roztwory badanych substancji roślinnych. Do określenia wartości MIC olejków i ekstraktów zastosowano metodę mikrorozcieńczeń w podłożu płynnym. Wśród badanych roślin tylko olejki eteryczne z Thymus syriacus i Cinnamomum zeylanicum i wyciąg z Laurus nobilis wykazały wysoką aktywność przeciwko szczepom B. melitensis. MIC50 dla T. syriacus, C. zeylanicum i L. nobilis wobec B. melitensis wynosiły odpowiednio 6,25, 3,125 i 6,25 μl/ml. Wśród badanych olejków eterycznych i wyciągów roślinnych, olejki eteryczne z T. syriacus i C. zeylanicum oraz wyciąg z L. nobilis były najbardziej aktywne. Co więcej, kombinacja T. syriacus - C. zeylanicum była bardziej skuteczna niż użycie każdego z tych olejków eterycznych osobno. Zatem olejki eteryczne z T. syriacus i C. zeylanicum oraz wyciąg z L. nobilis mogą być stosowane jako środki przeciwbakteryjne przeciwko B. melitensis.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2014
• Tom: 60
• Numer: 1
• Opis fizyczny: p.29-38,ref.

Twórcy

autor

Safi M.

• Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Syria

autor

Al-Mariri A.

• Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Syria

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Identyfikatory

DOI

10.2478/hepo-2014-0003