Detection and molecular identification of Entamoeba species in faecal samples from Duhok province, Kurdistan Region, Iraq

• Autorzy: Hasan H.K. , Mohammed A.B. , Mero W.M.S.
• Języki publikacji: EN

Abstrakty

EN

The study involved the estimation of the prevalence of Entamoebaspp. using microscopy and moleculartechniques among symptomatic outpatients from April 2021 to March, 2022. Stool samples were collected from 2592outpatients with amoebosis symptoms of both sexes and different ages (≤ l to 60). Also, 207 stool samples were takenrandomly from asymptomatic individuals and examined microscopically to detect infection with Entamoebaspp. thepositive specimens were used for molecular analysis with positive symptomatic samples targeting the 18S rRNA geneby nested PCR. Microscopically 21.68% (562/2592) were positive, for Entamoebaspp. Males showed highest infectionrate than females (67.43% vs 32.56%). Ages from 1–10 years showed the highest rate (54.09%), and urban inhabitanthad somewhat a higher rate than rural one (58.54% vs 41.45%) which was statistically non-significant (P>0.05). Amongasymptomatic individuals, 57% (61/107) were positive for Entamoebaspp. Nested PCR analysis yielded 73% positivesamples for Entamoebaspp. with a fragment size of 897 bp. Three fragment sizes were produced, for E. histolytica, E.disparand E. moshkovskiiwhich were 439, 174 and 553 bps, respectively. Single infection occurred with, E. histolyticain 46%, of symptomatic and 6% of asymptomatic cases, E. dispar in 38% of asymptomatic and 10% of symptomaticcases, E. moshkovskii, was reported at very low rate among both groups.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2024
• Tom: 70
• Numer: 2
• Opis fizyczny: p.91-101,fig.,ref.

Twórcy

autor

Hasan H.K.

• Department of Biology, College of Science, University of Zakho, Zakho 42002, Kurdistan Region, Iraq

autor

Mohammed A.B.

• Department of Biology, College of Science, University of Zakho, Zakho 42002, Kurdistan Region, Iraq

autor

Mero W.M.S.

• Department of Biology, College of Science, University of Zakho, Zakho 42002, Kurdistan Region, Iraq
• College of Science, Nawroz University, Duhok 42001, Kurdistan Region, Iraq

Identyfikatory

DOI

10.17420/ap7002.526