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2008 | 30 | 1 |
Tytuł artykułu

Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd

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Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Micropropagation system of Malus zumi was optimized by studying the influence of plant growth regulators and culture conditions. The axillary buds were used for mutiplication of in vitro shoot culture on agar Murashige and Skoog (1962) (MS) medium with combination of 1 mg l⁻¹ BAP, 0.5 mg l⁻¹ NAA or 0.5 mg l⁻¹ IAA or 0.5 mg l⁻¹ IBA under 16 h photoperiod. The shoot growth in culture was not significantly affected within a broad range (5.0–7.0) of initial medium pH. The highest shoot (13) was obtained on medium containing 1.0 mg l⁻¹ BAP and 0.5 mg l⁻¹ IAA. Welldeveloped shoots, 35–50 mm in length, were successfully rooted ex vitro at 86.3% by a 2-h-treatment with aqueous solution containing MS salts and 100 mg l⁻¹ IBA prior to their planting in growing substrate composed of soil and vermiculite (1:1 v/v). The survival rate of transplantation reached 88.0% when transferred to field condition.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
30
Numer
1
Opis fizyczny
p.129-132,fig.,ref.
Twórcy
autor
  • Department of Biology, Graduate University of Chinese Academy of Sciences, 1000049 Beijing, China
autor
  • Center for Agricultural Resources Research, Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, 050021 Shijiazhuang, China
autor
  • Department of Biological Engineering, School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), 100083 Beijing, China
autor
  • Department of Biology, Graduate University of Chinese Academy of Sciences, 1000049 Beijing, China
Bibliografia
  • Casado JP, Navarro MC, Utrilla MP, Martinez A, Jimenez J (2002) Micropropagation of Santolina canescens Lagasca and in vitro volatiles production by shoot explants. Plant Cell Tissue Organ Cult 69:147–153
  • Hofer M (1995) In-vitro-androgenesis in apple. Gartenbauwissenschaft 60:12–15
  • Liu Z, Li Z (2001) Micropropagation of Camptotheca acuminate Decaisne from axillary buds, shoot tips, and seed embryos in a tissue culture system. In Vitro Cell Dev Biol Plant 37:84–88
  • Martin KP (2003) Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep 21:415–420
  • MatsumotoT, Okunishi K, Nishida K, Noguchi M (1972) Effects of physical factors and antibiotics on the growth of higher plant cells in suspension culture. Agric Biol Chem J 36:2177–2183
  • Minocha SC (1987) pH of the medium and growth and metabolism of cells in culture. Cell Tissue Cult For 1:125–144
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nordstrom A, Tarkowski P, Tarkowska D, Norbaek R, Astot C, Dolezal K, Sandberg G (2004) Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin–cytokinin-regulated development. Proc Natl Acad Sci USA 101:8039–8044
  • Salvi ND, George L, Eapen S (2002) Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell Tissue Organ Cult 68:143–151
  • Samir CD (2004) Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. Plant Growth Regul 43:179–186
  • Sanchez MC, San-Jose MC, Ballester A, Vieitez AM (1996) Requirements for in vitro rooting of Quercus robur and Q. rubra shoots derived from mature trees. Tree Physiol 16:673–680
  • Scheewe P, Ketzel A (1994) In vitro screening for resistance against powdery mildew (Podosphaera leucotricha (Ell. et Ev.) Salm.) in apple. Zeitschrift Fuer Pflanzenkrankheiten and Pflanzenschutz 101:368–377
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Bibliografia
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bwmeta1.element.agro-6411f4c1-a226-444d-a5a8-da644ad03ea1
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