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Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd

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Micropropagation system of Malus zumi was optimized by studying the influence of plant growth regulators and culture conditions. The axillary buds were used for mutiplication of in vitro shoot culture on agar Murashige and Skoog (1962) (MS) medium with combination of 1 mg l⁻¹ BAP, 0.5 mg l⁻¹ NAA or 0.5 mg l⁻¹ IAA or 0.5 mg l⁻¹ IBA under 16 h photoperiod. The shoot growth in culture was not significantly affected within a broad range (5.0–7.0) of initial medium pH. The highest shoot (13) was obtained on medium containing 1.0 mg l⁻¹ BAP and 0.5 mg l⁻¹ IAA. Welldeveloped shoots, 35–50 mm in length, were successfully rooted ex vitro at 86.3% by a 2-h-treatment with aqueous solution containing MS salts and 100 mg l⁻¹ IBA prior to their planting in growing substrate composed of soil and vermiculite (1:1 v/v). The survival rate of transplantation reached 88.0% when transferred to field condition.
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  • Department of Biology, Graduate University of Chinese Academy of Sciences, 1000049 Beijing, China
  • Center for Agricultural Resources Research, Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, 050021 Shijiazhuang, China
  • Department of Biological Engineering, School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), 100083 Beijing, China
  • Department of Biology, Graduate University of Chinese Academy of Sciences, 1000049 Beijing, China
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