The C3-CAM intermediate plant Clusia fluminensis under well-watered at low light conditions opens stomata during the light period. In leaf extracts of this plant we have found two copper-zinc superoxide dismutases (CuZnSODs) and two manganese SODs: MnSOD-like protein (MnSOD II) and MnSOD I. Daily rhythm of the MnSOD I shows maximum activity during the afternoon hours and it is accompanied by only a very small tendency to increase in catalase (CAT) activity and lowering of citrate level.
Institute of Biology, Pedagogical University, Podbrzezie 3, 31-054 Krakow, Poland
Bibliografia
Aebi H (1984) Catalase in vitro. In: Methods in enzymology, vol 105. Academic, New York, pp 121–126
Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341
Bartosz G (1997) Oxidative stress in plants. Acta Physiol Plant 19:47–64
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Borland AM, Taybi T (2004) Synchronization of metabolic processes in plants with crassulacean acid metabolism. J Exp Bot 55:1255–1265
Borland AM, Griffiths H, Maxwell C, Broadmeadow MSJ, Fordham MC (1996) CAM induction in Clusia minor L. during the transition from wet to dry season in Trynidad: the role of organic acid speciation and decarboxylation. Plant Cell Environ 19:655–664
Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Castillo F (1996) Antioxidative protection in the inducible CAM plant Sedum album L. following the imposition of severe water stress and recovery. Oecologia 107:469–477
Dat J, Vandenabeele S, Vranová E, van Montagu M, Inzé D, van Breusegem F (2000) Dual action of active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
Desikan R, Mackerness SA-H, Hancock JT, Neill SJ (2001) Regulation of the Arabidopsis trancriptome by oxidative stress. Plant Physiol 127:159–172
Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine. Oxford University Press, New York, p 936
Herzog B, Grams TEE, Haag-Kerwer A, Ball E, Franco AC, Lüttge U (1999) Expression of modes of photosynthesis (C₃, CAM) in Clusia cruiva Camb. in a cerrado gallery forest transect. Plant Biol 1:357–364
Inzé D, van Montagu M (1995) Oxidative stress in plants. Curr Opin Biotechnol 6:153–158
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227:680–685
Libik M, Konieczny R, Surówka E, Miszalski Z (2005) Superoxide dismutase activity in organs of Mesembryanthemum crystallinum L. at different stages of CAM development. Acta Biol Cracov Ser Bot 47:199–204
Lüttge U (1999) One morphotype, three physiotypes: sympatric species of Clusia with obligate C₃ photosynthesis, obligate CAM and C₃-CAM intermediate behaviour. Plant Biol 1:138–148
Lüttge U (2000) Light-stress and crassulacean acid metabolism. Phyton 40:65–82
Lüttge U (2004) Ecophysiology of crassulacean acid metabolism (CAM). Ann Bot 93:629–652
de Mattos EA, Lüttge U (2001) Chlorophyll fluorescence and organic acid oscillation during transition from CAM to C₃-photosynthesis in Clusia minor L. (Clusiaceae). Ann Bot 88:457–463
Maxwell K, Borland AM, Haslam RP, Helliker BR, Roberts A, Griffiths H (1999) Modulation of rubisco activity during the diurnal phases of the crassulacean acid metabolism plant Kalanchoe daigremontiana. Plant Physiol 121:849–856
Miszalski Z, Ślesak I, Niewiadomska E, Baczek-Kwinta R, Lüttge U, Ratajczak R (1998) Subcellular localization and stress responses of superoxide dismutase isoforms from leaves in the C₃-CAM intermediate halophyte Mesembryanthemum crystallinum L. Plant Cell Environ 21:169–179
Miszalski Z, Kornas A, Gawronska K, Ślesak I, Niewiadomska E, Kruk J, Christian AL, Fischer-Schliebs E, Krish R, Lüttge U (2007) Ecophysiological aspects of mitochondrial MnSOD activity in species of Clusia with obligate C₃-photosynthesis and C₃/CAM intermediate behaviour. Biol Plant 51:86–92
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
Möllering H (1974) Malat. Bestimmung mit Malat-Dehydrogenase und Glutamat-Oxalacetat-Transaminase. In: Bergmeyer HU (ed) Methoden der Enzymologie. Academic, New York, pp 1636–1639
Möllering H (1985) Citrate. Determination with citrate lyase, MDH and LDH. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic, New York, pp 2–12
Niewiadomska E, Miszalski Z, Ślesak I, Ratajczak R (1999) Catalase activity during C₃-CAM transition in Mesembryanthemum crystallinum L. leaves. Free Rad Res 31:S251–S256
Niewiadomska E, Karpinska B, Romanowska E, Ślesak I, Karpinski S (2004) A salinity-induced C₃-CAM transition increases energy conservation in the halophyte Mesembryanthemum crystallinum L. Plant Cell Physiol 45:789–794
Roberts A, Borland AM, Griffiths H (1997) Discrimination processes and shifts in carboxylation during the phases of crassulacean acid metabolism. Plant Physiol 113:1283–1292
Salin ML, Bridges SM (1981) Localization of superoxide dismutases in chloroplasts from Brassica campestris. Z Pflanzenphysiol 99:37–45
Schröder Ch (2000) Vergleich von Isoenzymmustern verschidener Vertreter der Gattung Clusia L.: taxonomischer Vergleich und Versuch einer Stammbaumerstellung. University of Technology of Darmstadt, Germany, pp 1–35
Streller S, Krömer S, Wingsle G (1994) Isolation and purification of mitochondrial Mn-superoxide dismutase from the gymnosperm Pinus sylvestris L. Plant Cell Physiol 35(6):859–867
Ślesak I, Miszalski Z (2003) Superoxide dismutase-like protein from roots of the intermediate C₃-CAM plant Mesembryanthemum crystallinum in in vitro culture. Plant Sci 164:497–505
Ślesak I, Miszalski Z, Karpinska B, Niewiadomska E, Ratajczak R, Karpinski S (2002) Redox control of oxidative stress responses in the C₃-CAM intermediate plant Mesembryanthemum crystallinum. Plant Physiol Biochem 40:669–677
Ślesak I, Libik M, Karpinska B, Karpinski S, Miszalski Z (2007) The role of hydrogen peroxide in regulation of plant metabolism and cellular signalling in response to environmental stresses. Acta Biochim Pol (in press)
Van Breusegem F, Vranová E, Dat JF, Inzé D (2001) The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414
Winter K, Smith JAC (1996) Crassulacean acid metabolism. Current status and perspectives. In: Winter K, Smith JAC (eds) Crassulacean acid metabolism. Biochemistry, ecophysiology and evolution. Springer, Berlin, pp 389–426