Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 29 | 4 |
Tytuł artykułu

NaCl-induced changes in plasma membrane lipids and proteins of Zea mays L. cultivars differing in their response to salinity

Warianty tytułu
Języki publikacji
Two contrasting maize (Zea mays L.) cultivars, i.e., Giza 2 (salt tolerant) and Trihybrid 321 (salt sensitive), were grown hydroponically to study NaCl effect (100 mM) on root plasma membrane (PM) lipid and protein alterations. The PM total sterols of Trihybrid 321 were decreased while that of Giza 2 was increased in response to salt. Salt imposition had no significant effect on PM total glycolipids and proteins of both cultivars. The PM total phospholipids were increased in Trihybrid 321 but it did not change significantly in Giza 2 after salinity stress. Molecular percentage of PM phospholipids and fatty acids of both cultivars was different in absence (0 mM) and presence (100 mM) of salt. The most abundant phospholipids in untreated Trihybrid 321 PM were phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS), which changed into PG, PS, phosphatidylinositol (PI) and PC after salt treatment. However, the dominant phospholipids of the control PM of Giza 2 were PC, PE, PS and PG, which changed into PG, PE, PS and diphosphatidylglycerol (DPG) after salt imposition. Over 60% of the total fatty acids were saturated in control and salinized PM of both cultivars, which was increased after salt stress. The predominant fatty acid in the control and salinized PM of Trihybrid 321 was C18:1 and C17:0, respectively. However, in control and treated PM of Giza 2, the predominant fatty acid was C17:0 and C20:0, respectively. Qualitative and quantitative differences in PM protein patterns were found in both cultivars with and without salt. PM lipid changes enhanced membrane integrity, reflected in different ion accumulation (Mansour et al. 2005), and hence salt tolerance of Giza 2.
Słowa kluczowe
Opis fizyczny
  • Department of Botany, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
  • Department of Botany, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
  • Department of Botany, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
  • Department of Horticulture, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt
  • Ames BN (1966) Assays of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8:115–118
  • Apte SK, Bhagwat AA (2004) Salinity stress-induced proteins in two nitrogen-fixing Anabaena strains differentially tolerant to salt. J Biol Chem 279:12438–12447
  • Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 160:3–16
  • Bishop DG (1983) Functional role of plant membrane lipids. In: Thomson WW, Mudd JB, Gibbs M (eds) Biosynthesis and function of plant lipids. University of California, Riverside, pp 81–103
  • Blits KC, Gallagher JL (1990) Effect of NaCl on lipid content of plasma membrane isolated from roots and cell suspension cultures of the diocot halophyte kosteletzkya virginca (L.) Presl. Plant Cell Rep 9:156–159
  • Blumwald E (2000) Sodium transport and salt tolerance in plants. Curr Opin Cell Biol 12:431–434
  • Brown DJ, DuPont FM (1989) Lipid composition of plasma membrane and endomembranes prepared from roots of barley (Hordeum vulgare L.) effect of salt. Plant Physiol 90:955–961
  • Cogen V, Shinitzky M, Weber G, Nishida T (1973) Microviscosity and order in the hydrocarbon region of phospholipid and phospholipid-cholesterol dispersions determined with fluorescent probes. Biochemistry 12:521–528
  • Cramer GR, Lauchli A, Polito VS (1985) Displacement of Ca2+ by Na+ from the plasmalemma of root cells. A primary response to salt stress? Plant Physiol 79:207–211
  • Cramer GR, Alberico GJ, Schmidt C (1994) Leaf expansion limits dry matter accumulation of salt-stressed maize. Aust J Plant Physiol 21:663–674
  • Cullis PR, de Kruijff B (1979) Lipid polymorphism and the functional roles of lipid in biological membranes. Biochem Biophys Acta 559:399–420
  • De Kruijuff B, de Greef WJ, van Eyk RK, Demel RA, van Deenen LM (1973) The effect of different fatty acids and sterol composition on the erythritol flux through the cell membrane of Acholeplasma laidlawii. Biochem Biophys Acta 298:479–499
  • Deinstrop EH, Weinheim W (2000) Applied thin layer chromatography. Scottish Crop Research Institute, Dundee
  • Douglas TJ (1985) NaCl effects on sterol composition of plasma membrane-enriched preparation from citrus root plants. Plant Cell Environ 8:687–692
  • Douglas TJ, Walker RR (1984) Phospholipids, free sterols and adenosine triphosphatase of plasma membrane-enriched preparations from roots of Citrus genotypes differing in chloride exclusion ability. Physiol Plant 62:51–58
  • DuPont FM (1992) Salt induced changes in ion transport: regulation of primary pumps and secondary transporters. In: Cooke DT, Clarkson DT (eds) Transport and receptor proteins of plant membranes. Plenum Press, New York, pp 91–100
  • Epstein E (1972) Mineral nutrition of plants. Principles and perspective. Wiley, New York
  • Ericson MC, Alfinito SH (1984) Proteins produced during salt stress in tobacco cell culture. Plant Physiol 74:506–509
  • Gange J, Stamatatos L, Diacovo TSW, Yeagle PL, Silivus JP (1985) Physical properties and surface interactions of bilayer membranes containing N-methylate phosphatidyl-ethanolamine. Biochemistry 24:4400–4408
  • Goncalo A, Filho S, Ferreira BS, Dias JM, Queiroz KS, Branco A T, Bressan-Smith R E, Oliveira J G, Garcia B (2003) Accumulation of SALT protein in rice plants as response environmental stresses. Plant Sci 164:623–628
  • Henry RH, Cannon DC, Winkelman JW (1974) Clinical chemistry. Principles and techniques. Harper and Row, London
  • Hurkman WJ, Tanaka CK, DuPont FM (1988) The effects of salt stress on polypeptides in membrane fractions from barely roots. Plant Physiol 88:1263–1273
  • Iraki NM, Bressan RA, Carpita NC (1989) Extracellular polysaccharides and proteins of tobacco cell cultures and changes in composition associated with growth-limiting adaptation to water and saline stress. Plant Physiol 91:54–61
  • Kates M (1972) Techniques of lipidology. Isolation, analysis and identification of lipids. In: Work TS, Work E (eds) Laboratory techniques in biochemistry and molecular biology. North Holland, Amsterdam, pp 347–390
  • Kerkeb L, Donaire JP, Rodriguez-Rosales MP (2001) Plasma membrane H+-ATPase Activity is involved in adaptation of tomato to NaCl. Physiol Plant 111:483–490
  • Kononowicz AK, Raghothama KG, Casas AM, Nelson DE, Liu D, Narasimhan ML, LaRose PC, Singh NK, Bressan RA, Hasegawa PM (1994) Structure regulation and function of the osmotin gene. In: Cherry JH (ed) Biochemical and cellular mechanisms of stress tolerance in plants. Springer, Berlin, pp 381–413
  • Kuiper PJC (1984) Functioning of plant cell membranes under saline conditions: membrane lipid composition and ATPases. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants. Wiley, New York, pp 77–91
  • Kuiper PJC (1985) Lipids in grape roots in relation to chloride transport. Plant Physiol 43:1367–1371
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the heat bacteriophage T4. Nature 227:680–685
  • Larsson C, Moller I, Widell S (1990) Introduction to the plant plasma membrane. Its molecular composition and organization. In: Larsson C, Moller I (eds) The plant plasma membrane structure, function and molecular biology. Springer, Berlin, pp 1–15
  • Lauchli A (1990) Calcium, salinity and the plasma membrane. In: Leonard T, Hepler PK (eds) Calcium in plant growth and development. Am Soc Plant Physiol Symp, Rockville, pp 26–35
  • Lee AG (2003) Lipid-protein interactions in biological membranes: a structural prospective. Biochim Biophys Acta 1612:1–40
  • Levitt J (1980) Responses of plants to environmental stresses, II. Water, radiation, salt and other stresses. Academic, New York
  • Lin H, Wu L (1996) Effects of salt stress on root plasma membrane characteristics of salt tolerant and salt sensitive buffalo grass clones. Environ Exp Bot 36:239–254
  • Mansour MMF (1995) NaCl alteration of plasma membrane of Allium cepa epidermal cells. Alleviation by calcium. J Plant Physiol 145:726–730
  • Mansour MMF (1997) Cell permeability under salt stress. In: Jaiwl PKR, Singh RP, Gulati A (eds) Strategies for improving salt tolerance in higher plants. Science, Enfield, USA, pp 87–110
  • Mansour MMF, Stadelmann EJ (1994) NaCl-induced changes in protoplasmic characteristics of Hordeum vulgare cultivars differing in salt tolerance. Physiol Plant 91:389–394
  • Mansour MMF, Salama KHA (2004) Cellular basis of salt tolerance in plants. Environ Exp Bot 52:113–122
  • Mansour MMF, Stadelmann EJ, Lee-Stradelmann OY (1993) Salinity stress and cytoplasmic factors. A comparison of cell permeability and lipid partiality in salt sensitive and salt resistant cultivars and lines of Triticum aestivum and Hordeum vulgare. Physiol Plant 88:141–148
  • Mansour MMF, van Hasselt PR, Kuiper PJC (1994) Plasma membrane lipid alterations by NaCl in winter wheat roots. Physiol Plant 92:473–476
  • Mansour MMF, van Hasselt PR, Kuiper PJC (1998) Ca2+ and Mg2+-ATPase activities in winter wheat root plasma membranes as affected by NaCl stress during growth. J Plant Physiol 153:181–187
  • Mansour MMF, van Hasselt PR, kuiper PJC (2000) NaCl effects on root plasma membrane ATPase of salt tolerant wheat. Biol Plant 43:61–66
  • Mansour MMF, Al-Mutawa MM, Salama KHA, Abou Hadid AMF (2002) Effect of NaCl and polyamines on plasma membrane lipid of wheat roots. Biol Plant 45:235–239
  • Mansour MMF, Salama KHA, Al-Mutawa MM (2003) Transport proteins and salt tolerance in plants. Plant Sci 164:891–900
  • Mansour MMF, Salama KHA, Ali FZM, Abou Hadid AF (2005) Cell and plant responses to NaCl in Zea mays L. cultivars differing in salt tolerance. Gen Appl Plant Physiol 31:29–41
  • Mazliak P (1989) Membrane responses to environmental stresses: the lipids view point-introductory overview. In: Blacs PA, Gruiz K, Kramer T (eds) Biological role of plant lipids. Budapest and Plenum, New York, pp 505–509
  • Melchoir DL (1982) Lipid phase transitions and regulation of membrane fluidity in prokaryotes. Curr Top Membr Transp 17:263–315
  • Norberg P, Liljenberg C (1991) Lipid of plasma membranes prepared from oat root cells: effects of induced water-deficit tolerance. Plant Physiol 96:1136–1141
  • Opekarova M, Tanner W (2003) Specific lipid requirements of membranes—a putative bottleneck in heterologous expression. Biochem Biophys Acta 1610:11–22
  • Pitman MG, Lauchli A (2002) Global impact and agricultural ecosystem. In: Lauchli A, Luttge V (eds) Salinity: environment—plants—molecules. Kluwer, The Netherlands, pp 3–20
  • Plesofsky-Vig N (1996) The heat shock proteins and the stress response. In: Bramble R, Marzluf I (eds) The Mycota III, biochemistry and molecular biology. Springer, Berlin, pp 171–190
  • Racagni G, Pedranzani A S, Taleisnik E, Abdala G (2003) Effect of short-term salinity on lipid metabolism and ion accumulation in tomato roots. Biol Plant 47:373–377
  • Ramagopal S (1987) Salinity stress induced tissue-specific proteins in barley. Plant Physiol 84:324–331
  • Ritter D, Yopp JH (1993) Plasma membrane lipid composition of the halophilic cyanobacterium Aphanothece halophytica. Arch Microbiol 159:435–439
  • Rochester CP, Kjellbom P, Larsson C (1987) Lipid composition of plasma membranes from barley leaves and roots, spinach leaves and cauliflower inflorescences. Physiol Plant 71:257–263
  • Ros R, Cooke D, Burden R, James C (1990) Effects of herbicide MCPA and the heavy metals, cadmium and nickel on the lipid composition, Mg2+-ATPase activity and fluidity of plasma membranes from rice, Oryza sativa (cv. Bahia) shoots. J Exp Bot 41:457–462
  • Russell NJ (1989) Functions of lipids: structural roles and membrane functions. In: Ratledge C, Wilkinson SC (eds) Microbial lipids. Academic, London, pp 279–365
  • Sandstorm R, Cleland R (1989) Selective delipiation of the plasma membrane by surfactant. Plant Physiol 90:1524–1531
  • Scarpa A, De Gier J (1971) Cation permeability as a function of the composition of the lipid bilayer. Biochem Biophys Acta 241:787–797
  • Simon EW (1974) Phospholipids and plant membrane permeability. New Phytol 73:377–420
  • Singh NK, Bracken CA, Hasegawa PM, Handa AK, Buckel S, Hermodoson MA, Pfankoch F, Regnier FE, Bressan RA (1987) Characterization of osmotin. A thaumatin-like protein associated with osmotic adjustment in plant cells. Plant Physiol 85:529–536
  • Stadelmann EJ, Lee-Stadelmann OY (1989) Passive permeability. Method Enzymol 174:246–266
  • Sung D, Kaplan F, Guy C (2001) Plant Hsp70 molecular chaperons: protein structure, gene family, expression and function. Physiol Plant 113:443–451
  • Tal M (1985) Genetics of salt tolerance in higher plants: theoretical and practical consideration. Plant Soil 89:199–226
  • Testerink C, Munnik T (2005) Phosphatidic acid: a multifunctional stress signaling lipid in plants. Trends Plant Sci 10:368–375
  • Thiemann B, Imhoff JF (1991) The effect of salt on the lipid composition of Ectothiorhodospira. Arch Microbiol 56:376–384
  • Tyerman SD, Skerrett I M (1999) Root ion channels and salinity. Sci Hort 78:175–235
  • Van Zoelen EJ, DE Jesus CH, De Tonge E, Mulder M, Block MC, De Gier G (1978) Non-electrolyte permeability as a tool for studying membrane fluidity. Biochim Biophys Acta 511:335–347
  • Vazquez-Duhalt R, Alcaraz-Meléndez L, Greppin H (1991) Variation in polar-group content in lipids of cowpea (Vigna unguiculata) cell cultures as a mechanism of haloadaptation. Plant Cell Tissue Organ Cult 26:83–88
  • Wang X (2004) Lipid signaling. Curr Opin Plant Biol 7:329–336
  • Wu J, Seliskar DM, Gallagher JL (1998) Stress tolerance in the Marsh plant Spartina patens: impact of NaCl on growth and root plasma membrane lipid composition. Physiol Plant 102:307–317
  • Wu J, Seliskar DM, Gallagher JL (2005) The response of plasma membrane lipid composition in callus of the halophyte, Spartina patens, to salinity stress. Am J Bot 92:852–858
  • Yang YL, Guo JK, Zhang F, Zhao LQ, Zhang LX (2004) NaCl-induced changes of the H+-ATPase in root plasma membrane of two wheat cultivars. Plant Sci 166:913–918
  • Zhang JS, Xie C, Li ZY, Chen SY (1999) Expression of the plasma membrane H+-ATPase gene in response to salt stress in a rice salt-tolerant mutant and its original variety. Theor Appl Genet 99:1006–1011
  • Zhu B, Chen TH, Li PH (1995) Expression of three osmotin-like protein genes in response to osmotic stress and fungal infection in potato. Plant Mol Biol 28:17–26
  • Zidan I, Shaviv A, Ravina I, Neumann PM (1992) Does salinity inhibit maize leaf growth by reducing tissue concentrations of essential mineral nutrients? J Plant Nutr 15:1407–1419
  • Zlatkis A, Zak B (1969) Study of a new cholesterol reagent. Ana1 Biochem 29:143–148
  • Zongli W, Xiaozhong L, Zhixia W (1987) Physiological studies on salt tolerance in rice. II. Changes in plasmalemma permeability and its relation with membrane lipid peroxidation in leaves under salt stress. J Agric Sci 3:1–9
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.