Strawberry anthocyanin determination by pH differential spectroscopic method - how to get true results?

• Autorzy: Tonutare T. , Moor U. , Szajdak L.

Warianty tytułu

PL

Określenie poziomu antocyjanów w truskawkach metodą spektroskopii różnicowej – jak uzyskać prawdziwe wyniki?

• Języki publikacji: EN

Abstrakty

EN

The aim of the research was to analyse the weaknesses of the pH differential method for strawberry anthocyanin determination. The work is based on practical experiments with 12 strawberry cultivars and on analysis of published papers. We used following molar absorption coefficients ( ƹ values): 26900 and 29600 M⁻¹ cm⁻¹ for cyanidin 3 glycoside (C3g) and 15600, 22400, 27300 and 36000 M⁻¹ cm⁻¹ for pelargonidin 3 glycoside (P3g). In order to show how the calculated value of total anthocyanins depends on the predominant anthocyanin used for the calculations, we compared the results of spectroscopic and chromatographic analysis. Present research demonstrated that different ƹ values may influence the results of total anthocyanins even more than cultivar properties. The most frequently used ƹ values 26900 M⁻¹ cm⁻¹ and 29600 M⁻¹ cm⁻¹ gave underestimated values. C3g was present in minor amounts in all cultivars. Conclusively, P3g with the ƹ = 15600 M⁻¹ cm⁻¹ should be used for ensuring most precise estimation of total anthocyanin content in strawberries.

PL

Celem niniejszego badania była analiza słabości metody różnicowego pH dla ustalenia antocyjanów w truskawkach. Praca powstała na podstawie doświadczeń na 12 odmianach truskawek oraz na podstawie analizy opublikowanych opracowań. Użyto następujących współczynników absorpcji molowej (wartości ƹ): 26900 i 29600 M⁻¹cm⁻¹ dla cyjanidyno 3-glukozydu (C3g) oraz 15600, 22400, 27300 i 36000 M⁻¹cm⁻¹ dla 3-glukozydu pelargonidyny (P3g). Aby wykazał, w jaki sposób wyliczona całkowita wartość antocyjanów zależy od dominujących antocyjanów, porównano wyniki analizy spektroskopowej i chromatograficznej. W niniejszym badaniu wykazano, że różne wartości ƹ mogą wpływać na wyniki całkowitych antocyjanów nawet bardziej niż cechy odmiany. Najczęściej stosowane wartości ƹ 26900 M⁻¹cm⁻¹ oraz 29600 M⁻¹cm⁻¹ dały wartości zaniżone. C3g był obecny w niewielkich ilościach we wszystkich odmianach. Podsumowując, można stwierdzić, że P3g o wartości ƹ = 15600 M⁻¹cm⁻¹ należy stosować, aby zapewnić najbardziej precyzyjne oszacowanie całkowitej zawartości antocyjanów w truskawkach.

Słowa kluczowe

EN

strawberry; Fragaria x ananassa; anthocyanin; determination; molar absorption coefficient; pelargonidin; plant pigment

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 3
• Opis fizyczny: p.35-47,fig.,ref.

Twórcy

autor

Tonutare T.

• Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estoni

autor

Moor U.

• Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia

autor

Szajdak L.

• Polish Academy of Sciences, Poznan, Poland

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