Influence of medium salt strength and nitrogen source on biomass and metabolite accumulation in adventitious root cultures of Pseudostellaria heterophylla

• Autorzy: Yin S. , Liang Y. , Gao W. , Wang J. , Jing S. , Zhang Y. , Liu H.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effects of medium salt strength and ammonia/nitrate ratio on biomass production and metabolites accumulation of adventitious root. The medium with full-strength Murashige and Skoog (MS) reached the highest growth rate (16.77), and the contents of saponin and polysaccharide reached the peak (i.e., 0.65 and 24.85 %) at 3/4 MS and 1 MS, respectively. In case of ammonia/nitrate ratio, a NH4+/NO3- ratio of 20:40 was optimal for the production of biomass and polysaccharide (23.27 %). In contrast, the content of saponin achieved the optimum (0.74 %) at a NH4+/NO3 - ratio of 30:30. In 5-L balloon-type bubble bioreactor (BTBB) cultures, an approximately 23-fold increase in biomass was recorded. The fresh weight (FW) and dry weight (DW) were 72.78 and 6.79 g per bioreactor with the contents of saponin (0.62 %) and polysaccharide (17.32 %), respectively. It indicated potential application to produce adventitious roots of pseudostellaria heterophylla with bioreactors on large scale in commercial.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 08
• Opis fizyczny: p.2623-2628,fig.,ref.

Twórcy

autor

Yin S.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China

autor

Liang Y.

• Tongren Polytechnic, Tongren 554300, China

autor

Gao W.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China

autor

Wang J.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China

autor

Jing S.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China

autor

Zhang Y.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China

autor

Liu H.

• Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China

Bibliografia

• Ahn JK, Lee WY, Park EJ (2009) Effect of nitrogen on eleutheroside production and adventitious root growth in Eleutherococcus koreanum Nakai bioreactor cultures. J Korean For Soc 98:363–369
• Baque MA, Lee EJ, Paek KY (2010) Medium salt strength induced changes in growth, physiology and secondary metabolite content in adventitious roots of Morinda citrifolia: the role of antioxidant enzymes and phenylalanine ammonia lyase. Plant Cell Rep 29:685–694
• Baque MA, Moh SH, Lee EJ, Zhong JJ, Paek KY (2012) Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol Adv 30:1255–1267
• Chen JH, Xie MY, Nie SP, Wang YX, Peng RH (2005) Determination of polysaccharides in Panax quinquefolium L. J Food Sci Biotechnol 24:72–76
• Choi SM, Son SH, Yun SR, Kwon OW, Seon JH, Paek KY (2000) Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell Tiss Org cult 62:187–193
• Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010a) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresource Technol 101:4708–4716
• Cui XH, Murthy HN, Wu CH, Paek KY (2010b) Adventitious root suspension cultures of Hypericum perforatum: effect of nitrogen source on production of biomass and secondary metabolites. In Vitro Cell Dev Biol Plant 46:437–444
• Fu XS, Liu XH, Xu H, Zhou YZ, Chen F (2012) The present situation and development trend of Pseudostellaria heterophylla. Chin J New Drug 21:757–760
• Han C, Chen JH, Liu J, Lee FSC, Wang XR (2007) Isolation and purification of Pseudostellarin B (cyclic peptide) from Pseudostellaria heterophylla (Miq.) Pax by high-speed counter-current chromatography. Talanta 71:801–805
• Huang T, Gao WY, Wang J (2010) Selection and optimization of a high-producing tissue culture of Panax ginseng C. A. Meyer. Acta Physiol Plant 32:765–772
• Kim JH, Chang EJ, Oh HI (2005) Saponin production in submerged adventitious root culture of Panax ginseng as affected by culture conditions and elicitors. Asia Pac J Mol Biol and Biotech 13:87–91
• Kronzucker HJ, Siddiqi MY, Glass ADM, Kirk GJD (1999) Nitrateammonium synergism in rice: a subcellular flux analysis. Plant Physiol 119:1041–1104
• Lee EJ, Paek KY (2012a) Effect of nitrogen source on biomass and bioactive compound production in submerged cultures of Eleutherococcus koreanum Nakai adventitious roots. Biotechnol Prog 28:508–514
• Lee EJ, Paek KY (2012b) Enhanced productivity of biomass and bioactive compounds through bioreactor cultures of Eleutherococcus koreanum Nakai adventitious roots affected by medium salt strength. Ind Crops Prod 36:460–465
• Lin CF, Zhong AQ, Wei ZP (2005) Study on in vitro culture and rapid propagation of Pseudostellaria heterophylla growing point. Fujian J Agr Sci 20:113–117
• Liu XH, Tan XH, Zeng YP (2007) Comparison of quality of Pseudostellaria heterophylla from different habitats. Res Pract Chin Med 22:36–38
• Ma XM, Wu CF, Wang GR (2011) Application of artificial seeds in rapid multiplication of Pseudostellaria heterophylla. Afr J Biotech 10:15744–15748
• Panda AK, Mishra S, Bisaria VS (1992) Alkaloid production by plant cell cultures of Holarrhena antidysenterica: Effects of major nutrients. Biotechnol Bioeng 39:1043–1051
• Qin MJ, Yu YB, Huang WZ, Zhu Y, Yan L, Pu SB (2005) Quality assay of Pseudostellaria heterophylla collected from different regions. Res Pract Chin Med 19:29–32
• Shen Y, Han C, Liu JD, Liu A, Ji XW, Liu CP (2008) Analysis of volatile components of Pseudostellaria heterophylla (Miq.) Pax by microwave-assisted solvent extraction and GC–MS. Chromatographia 68:679–682
• Wang GR, Qi NM (2010) Influence of mist intervals and aeration rate on growth and second metabolite production of Pseudostellaria heterophylla adventitious roots in a siphon-mist bioreactor. Biotechnol Bioproce E 15:1059–1064
• Wang J, Gao WY, Zuo BM, Liu H, Zhang LM, Huang LQ (2012) Gradually scale-up culture in a bioreactor promotes radical scavenging activity of Panax ginseng (C. A. Meyer) adventitious roots on 1, 1-diphenyl-2-picrylhydrazyl. Plant Growth Regul 67:101–105
• Wen XS, Zhao HY, Li YY, Huo DL (2003) Studies on seed dormancy of Pseudostellaria heterophylla. Chin Tradit Herb Drug 34:762–763
• Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199
• Wu SQ, Lian ML, Gao R, Park SY, Piao XC (2011) Bioreactor application on adventitious root culture of Astragalus membranaceus. In Vitro Cell Dev Biol Plant 47:719–724
• Yamamoto O, Kamura K (1997) Production of saikosaponin in cultured roots of Bupleurum falcatum L. Plant Tiss Cult Biotechnol 3:138–147
• Ye Z, Wang YY, Tian HQ (2009) Regenration of plantlets and tetraploidy induction in Pseudostellaria heterophylla. Acta Biol Cracov Bot 51:13–18
• Yu KW, Hahn EJ, Paek KY (2000) Production of adventitious ginseng roots using bioreactors. Korean J Plant Tiss Cult 27:309–315
• Zhang CL, Zheng XX (2011) Effects of polysaccharides from Pseudostellaria heterophylla on exercise endurance capacity and oxidative stress in forced swimming rats. Sci Res Essays 6:2360–2365
• Zhang YH, Zhong JJ, Yu JT (1996) Effect of nitrogen source on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnol Prog 12:567–571
• Zhang J, Gao WY, Wang J, Li XL, Xiao PG (2011) Improvement of growth and periplocin yield of periploca sepium adventitious root cultures by altering nitrogen source supply. Chin Herb Med 3:226–231

Uwagi

rekord w opracowaniu