Effect of matrix metalloprotease inhibition on short and long term plasticity of mossy fiber-CA3 synapses

• Autorzy: Wojtowicz T. , Piotrowska A. , Podborska-Okolow M. , Dziegiel P. , Kaczmarek L. , Mozrzymas J.
• Języki publikacji: EN

Abstrakty

EN

Matrix metalloproteinases (MMPs) are a large family of extracellularly-acting endopeptidases. MMPs were shown to play an important role in hippocampal plasticity phenomena such as LTP, learning and memory. In particular, in Shaffer collateral/ CA1 (Sch/CA1) synapses, MMPs are crucial for maintenance of long term plasticity requiring NMDA receptor (NMDAR) activation. However, it is not known whether MMPs affect plastic changes in mossy-fi ber/CA3 (mf/CA3) synapses, where LTP is basically presynaptic and NMDAR-independent. Field potential (fEPSPs) recordings in CA3 region (mf stimulation) revealed no effect of FN439 (panmetalloprotease inhibitor) on the short-term plasticity but the late phase of LTP was abolished by this agent similar to Sch/CA1 pathway. Since maintenance of LTP in Sch/CA1 was specifi cally dependent on MMP-9 activity, we have performed immunostaining for MMP-9 in mf/CA3 pathway. Semi-quantitative analysis revealed that induction of LTP resulted in several fold increase in MMP-9 immunoreactivity within hilar and CA3 region relative to control slices. We conclude that MMPs are crucial for long- but not short-term plasticity in mf/CA3 synapses and that LTP induction in this pathway is accompanied by a robust increase in MMP-9 immunoreactivity. It seems thus that consolidation phase of LTP is critically dependent on MMP activity in the two major hippocampal pathways (Sch/CA1 and mf/CA3) where LTP induction mechanisms show profound differences. Support: Grant No. P-N/030/2006.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.307

Twórcy

autor

Wojtowicz T.

• Laboratory of Neuroscience,,Department of Biophysic, Wroclaw Medical University, Wroclaw, Poland

autor

Piotrowska A.

• Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland

autor

Podborska-Okolow M.

• Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland

autor

Dziegiel P.

• Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland

autor

Kaczmarek L.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Mozrzymas J.

• Laboratory of Neuroscience,,Department of Biophysic, Wroclaw Medical University, Wroclaw, Poland