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2012 | 47 | 2 |

Tytuł artykułu

Isolation of bacteria from ectomycorrhizae of Tuber aestivum Vittad.

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Fifteen different cultivation media were used to isolate bacteria with the idea to obtain taxa specifically associated with ectomycorrhizae of Tuber aestivum. Ectomycorrhizae were collected at the sampling points previously analyzed for bacterial molecular diversity. We isolated 183 bacterial strains and identified them on the basis of the partial sequence of 16S rDNA. Out of these isolates, only 4 corresponded to operational taxonomic units significantly associated with T. aestivum ectomycorrhizae in previous molecular study. Preliminary study of the effect of 12 selected isolates on growth of T. aestivum mycelium showed no stimulation and one isolate induced the damage of hyphae. Different isolation strategy has to be developed to increase the probability of cultivation of potentially important components of T. aestivum mycorrhizosphere.

Słowa kluczowe

Wydawca

-

Czasopismo

Rocznik

Tom

47

Numer

2

Opis fizyczny

p.155-160,fig.,ref.

Twórcy

autor
  • Institute of Microbiology ASCR, Videnska 1083, CZ-14220 Prague 4, Czech Republic
autor
  • Institute of Microbiology ASCR, Videnska 1083, CZ-14220 Prague 4, Czech Republic

Bibliografia

  • Bedini S., Bagnoli G., Sbrana C., Leporini C., Tola E., Dunne C., Filippi C., D’Andrea F., O’Gara F., Nuti M.P. 1999. Pseudomonads isolated from within fruit bodies of Tuber borchii are capable of producing biological control or phytostimulatory compounds in pure culture. Symbiosis 26: 223–236.
  • Chalot M., Brun A. 1998. Physiology of organic nitrogen acquisition by ectomycorrhizal fungi and ectomycorrhizas. FEMS Microbiol. Rev. 22: 21–44.
  • Citterio B., Malatesta M., Battistelli S., Marcheggiani F., Baffone W., Saltarelli R., Stocchi V., Gazzanelli G. 2001. Possible involvement of Pseudomonas fluorescens and Bacillaceae in structural modifications of Tuber borchii fruit bodies. Can. J. Microbiol. 47: 264–268.
  • Deveau A., Palin B., Delaruelle C., Peter M., Kohler A., Pierrat J.C., Sarniguet A., Garbaye J., Martin F., Frey-Klett P. 2007. The mycorrhiza helper Pseudomonas fluorescens BBc6R8 has a specific priming effect on the growth, morphology and gene expression of the ectomycorrhizal fungus Laccaria bicolor S238N. New Phytol. 175: 743–755.
  • Dowd S.E., Callaway T.R., Wolcott R.D., Sun Y., McKeehan T., Hagevoort R.G., Edrington T.S. 2008. Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). BMC Microbiol. 8: 125.
  • Garbaye J. 1994. Helper bacteria: a new dimension to the mycorrhizal symbiosis. New Phytol. 128: 197–210.
  • Gryndler M., Soukupová L., Hršelová H., Gryndlerová H., Borovička J., Streiblová E., Jansa J. 2012. A quest for indigenous truffle helper prokaryotes. Environ. Microbiol. Rep., OnlineFirst, DOI: 10.1111/1758-2229.12014
  • Jarerat A., Pranamida H., Tokiwa Y. 2002. Poly(L-lactide)-degrading activity in various actinomycetes. Macromolec. Biosci. 2: 420–428.
  • Linderman R.G. (1988) Mycorrhizal interactions with the rhizosphere microflora: the mycorrhizosphere effect. Phytopathology 78: 366–371.
  • Mello A., Miozzi L., Vizzini A., Napoli C., Kowalchuk G., Bonfante P. 2010. Bacterial and fungal communities associated with Tuber magnatum-productive niches. Plant Biosyst. 144: 323–332.
  • Murat C., Vizzini A., Bonfante P., Mello A. 2005. Morphological and molecular typing of the below-ground fungal community in a natural Tuber magnatum truffle-ground. FEMS Microbiol. Lett. 245: 307–313.
  • Napoli C., Mello A., Bonfante P. 2008. Dissecting the rhizosphere complexity: the truffle-ground study case. Rend. Lincei Sci. Fis. Nat. 19: 241–259.
  • Sukkhum S., Tokuyama S., Tamura T., Kitpreechavanich V. 2009. A novel poly(L-lactide) degrading actinomycetes isolated from Thai forest soils, phylogenic relationship and enzyme characterization. J. Gen. Appl. Microbiol. 55: 459–467.
  • Sbrana C., Agnolucci M., Bedini S., Lepera A., Toffanin A., Giovannetti M., Nuti M.P. 2002. Diversity of culturable bacterial populations associated to Tuber borchii ectomycorrhizas and their activity on T. borchii mycelial growth. FEMS Microbiology Letters 211: 195–202.
  • Zacchi L., Vaughan-Martini A., Angelini P. 2003. Yeast distribution in a truffle-field ecosystem. Ann. Microbiol. 53: 275–282.

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Bibliografia

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