MMP-2 plays an important role during the early acute developmental phase of oligofructose-induced equine laminitis

• Autorzy: Li X. , Jiang R. , Wang G. , Li Y. , Fan X. , Liu X. , Wang J. , Pan J. , Gao L.
• Języki publikacji: EN

Abstrakty

EN

The study was conducted on 24 Mongolian horses, with oligofructose-induced equine laminitis (10 g/kg b.w.). The objective of the study was to investigate the relationships among matrix metalloproteinase 2 (MMP-2), P38 mitogen-activated protein kinases (P38 MAPK), tissue inhibitor of metalloproteinase 2 (TIMP-2), lipopolysaccharides (LPS), and tumour necrosis factor-α (TNF-α) during acute developmental phase of laminitis, and to determine whether there are any characteristic tendencies. Moreover, plasma concentrations of LPS and TNF- α were measured in order to determine the time of leukocytes' activation. Eleven of the 12 horses showed clinical signs of laminitis. The contents of MMP-2 and P38 MAPK increased significantly from 8 h to 64 h, and the content of TIMP-2 decreased significantly at the same time. Plasma LPS concentrations increased significantly between 8 h and 20 h and reached a peak of 0.024 ± 0.009 EU/mL (equivalent to 3.04 ±1.19 pg/mL) at 12 h. TNF- α concentration increased between 20 h and 36 h. This data indicates that MMP-2 plays an important role during the early acute developmental phase of oligofructose-induced equine laminitis.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 1
• Opis fizyczny: p.149-153,fig.,ref.

Twórcy

autor

Li X.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Jiang R.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Wang G.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Li Y.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Fan X.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Liu X.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Wang J.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Pan J.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

autor

Gao L.

• College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

Bibliografia

• 1. Bailey S.R., Adair H.S., Reinemeyer C.R., Morgan S.J., Brooks A.C., Longhofer S.L., Elliott J.: Plasma concentrations of endotoxin and platelet activation in the developmental stage of oligofructose-induced laminitis. Vet Immunol Immunopathol 2009, 129, 167-173.
• 2. Belknap J.K.: Black walnut extract: an inflammatory model. Vet Clin North Am Equine Pract 2010, 26, 95-101.
• 3. Brooks A.C., Menzies-Gow N.J., Wheeler-Jones C., Bailey S.R., Cunningham F.M., Elliott J.: Endotoxin-induced activation of equine platelets: evidence for direct activation of p38 MAPK pathways and vasoactive mediator production. Inflamm Res 2007, 56, 154-161.
• 4. Collins S.N., van Eps A.W., Pollitt C.C., Kuwano A.: The lamellar wedge. Vet Clin North Am Equine Pract 2010, 26, 179-195.
• 5. Antonella D., Rosa D., Michele Q., Vittorio M., Porzia C., Angelo P., Vito M.G., Cosimo D.G., Gennaro G.C., Eufemia S., Rosanna M., Antonia B., Vito F.: Clinical and prognostic role of circulating MMP-2 and its inhibitor TIMP-2 in HCC patients prior to and after trans-hepatic arterial chemo-embolization. Clin Biochem 2013, 47, 184-190.
• 6. De Laat M.A., Clement C.K., McGowan C.M., Sillence M.N., Pollitt C.C., Lacombe V.A.: Toll-like receptor and proinflammatory cytokine expression during prolonged hyperinsulinaemia in horses: Implications for laminitis. Vet Immunol Immunopathol 2014, 157, 78-86.
• 7. De Laat M.A., Kyaw-Tanner M.T., Nourian A.R., McGowan C.M., Sillence M.N.. Pollitt C.C.: The developmental and acute phases of insulin-induced laminitis involve minimal metalloproteinase activity. Vet Immunol Immunopathol 2011, 140, 275-281.
• 8. De Laat M.A., Patterson-Kane J.C., Pollitt C.C., Sillence M.N., McGowan C.M.: Histological and morphometric lesions in the pre-clinical, developmental phase of insulin-induced laminitis in Standardbred horses. Vet J 2013, 195, 305-312.
• 9. Donnini S., Morbidelli L., Taraboletti G., Ziehe M.: ERKI-2 and p38 MAPK regulate MMP/TIMP balance and function in response to thrombospondin-l fragments in the microvascular endothelium. Life Sci 2004, 74, 2975-2985.
• 10. Dugdale A.H., Grove-White D., Curtis G.C., Harris P.A., Argo C.M.: Body condition scoring as a predictor of body fat in horses and ponies. Vet J 2012, 194, 173-178.
• 11. Eades S.C.: Overview of current laminitis research. Vet Clin North Am Equine Pract 2010, 26, 51-63.
• 12. Eps A.V., Pollitt C.C.: Equine laminitis induced with oligofructose. Equine Vet J 2006, 38, 203-208.
• 13. Howard E.W., Crider B.J., Updike D.L., Bullen E.C., Parks E.E., Haaksma C.J., Sherry D.M., Tomasek J.J.: MMP-2 expression by fibroblasts is suppressed by the myofibroblast phenotype. Exp Cell Res 2012, 318, 1542-1553.
• 14. Loftus J.P., Johnson P.J., Belknap J.K., Pettigrew A., Black S.J.: Leukocyte-derived and endogenous matrix metalloproteinases in the lamellae of horses with naturally acquired and experimentally induced laminitis. Vet Immunol Immunopathol 2009, 129, 221-230.
• 15. Longland A.C., Byrd B.M.: Pasture nonstructural carbohydrates and equine laminitis. J Nutr 2006, 136, 2099-2102.
• 16. Menzies-Gow N.J., Bailey S.R., Katz L.M., Marr C.M., Elliott J.: Endotoxin-induced digital vasoconstriction in horses: associated changes in plasma concentrations of vasoconstrictor mediators. Equine Vet J 2004, 36, 273-278.
• 17. Moore J.N., Garner H.E., Berg J.N., Sprouse R.F.: Intracecal endotoxin and lactate during the onset of equine laminitis: a preliminary report. Am J Vet Res 1979, 40, 722-723.
• 18. Morris D.D.: Endotoxemia in horses: a review of cellular and humoral mediators involved in its pathogenesis. J Vet Intern Med 1991, 5, 167-181.
• 19. Pollitt C.C.: The anatomy and physiology of the suspensory apparatus of the distal phalanx. Vet Clin North Am Equine Pract 2010, 26, 29-49.
• 20. Pollitt C.C., Visser M.B.: Carbohydrate alimentary overload laminitis. Vet Clin North Am Equine Pract 2010, 26, 65-78.
• 21. Pons M., Cousins S.W., Alcazar O., Striker G.E., Marin-Castaño M.E.: Angiotensin Il-lnduced MMP-2 activity and MMP-14 and Basigin protein expression are mediated via the angiotensin II receptor type I-mitogen-activated protein kinase I pathway in retinal pigment epithelium: implications for age-related macular degeneration. Am J Pathol 2011, 178, 2665-2681.
• 22. Radziwon-Balicka A., Ramer C., Moneada de la Rosa C., Zielnik-Drabik B., Jurasz P.: Angiostatin inhibits endothelial MMP-2 and MMP-14 expression: a hy poxia specific mechanism of action. Vascul Pharmacol 2013, 58, 280-291.
• 23. Stewart A.J., Pettigrew A., Cochran A.M., Belknap J.K.: Indices of inflammation in the lung and liver in the early stages of the black walnut extract model of equine laminitis. Vet Immunol Immunopathol 2009, 129, 254-260.
• 24. Tadros E.M., Frank N., Horohov D.W.: Inflammatory cytokine gene expression in blood during the development of oligofructose-induced laminitis in horses. J Equine Vet Sci 2013, 33, 802-808.
• 25. Taylor D., Hood D.M., Wagner I.P.: Short-term effect of therapeutic shoeing on severity of lameness in horses with chronic laminitis. Am J Vet Res 2002, 63, 1629-1633.
• 26. Toth F., Frank N., Chameroy K.A., Boston R.C.: Effects of endotoxaemia and carbohy drate overload on glucose and insulin dynamics and the development of laminitis in horses. Equine Vet J 2009, 41, 852-858.
• 27. Visser M.B., Pollitt C.C.: Lamellar leukocyte infiltration and involvement of IL-6 during oligofructose-induced equine laminitis development. Vet Immunol Immunopathol 2011, 144, 120-128.
• 28. Wang L., Pawlak E.A., Johnson P.J., Belknap J.K., Alfandari D., Black S.J.: Expression and activity of metalloproteinases in the digital laminae of horses with starch gruel-induced laminitis. J Equine Vet Sci 2011, 31, 566.
• 29. Wilkinson J.M., Davidson R.K., Swingler T.E., Jones E.R., Corps A.N., Johnston P., Riley G.P., Chojnowski A.J., Clark I.M.: MMP-14 and MMP-2 are key metalloproteases in Dupuytren's disease fibroblast-mediated contraction. Biochim Biophys Acta 2012, 1822, 897-905.
• 30. Yang J., Wang G., Gao C., Shao G., Kang N.: Effects of hyperbaric oxygen on MMP-2 and MMP-9 expression and spinal cord edema after spinal cord injury. Life Sci 2013, 93, 1033-1038.

Identyfikatory

DOI

10.1515/bvip-2015-0022