Immunohistochemical detection of Lawsonia intracellularis in tissue section from pigs

• Autorzy: Szczotka A. , Stadejek T. , Zmudzki J. , Nowak A. , Osinski Z. , Pejsak Z.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to develop an immunohistochemical method (IHC) for detection of Lawsonia intracellularis (L. intracellularis) in formalin-fixed, paraffin embedded sections of intestines from pigs and to implement this method in differential diagnosis of swine diseases with diarrhea in postweaning pigs. The study was conducted on 165 sections of intestines (ileum, caecum and colon) collected from 76 pigs, representing 42 Polish pig farms. The animals included in the analysis suffered from diarrhea, with bloody or grey to brown feces, and were suspected of porcine proliferative enteropathy (PPE). Sections of intestines were analyzed for the presence of L. intracellularis by polymerase chain reaction (PCR) and IHC. Among 165 intestinal samples from pigs with diarrhea, L. intracellularis DNA was detected by PCR in 33 (20.0%) samples. In this group, 30 samples (18.2% of all the samples tested) were also found positive in IHC, while only 3 (1.8%) were IHC-negative. One hundred thirty-two (80.0%) samples were negative in both tests. The PCR- and IHC-positive samples originated from 11 pigs, 4- to 20-week old, from 8 farms. L. intracellularis antigen was visualized by IHC mostly in intestinal crypts and/or in mononuclear cells of the lamina propria). The positive signal in epithelial cells was observed close to the luminal borders, creating typical specifically stained rims around the crypt lumina.ą The results of the present study further confirm the usefulness of IHC in the detection of L. intracellularis antigen in the intestinal tissues.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 4
• Opis fizyczny: p.531-538,fig.,ref.

Twórcy

autor

Szczotka A.

• Department of Swine Diseases, National Veterinary Research institute, Partyzantow 57, 24-100 Pulawy, Poland

autor

Stadejek T.

autor

Zmudzki J.

autor

Nowak A.

autor

Osinski Z.

autor

Pejsak Z.

Bibliografia

• Boesen HT, Jensen TK, Jungersen G, Riber U, Boye M, Mzller K (2005) Development, characterization and diagnostic application of a monoclonal antibody specific for a proteinase K resistant Lawsonia intracellularis antigen. Vet Microbiol 105: 199-206.
• Brandt D, Kaim U, Baumgartner W, Wendt M (2010) Evaluation of Lawsonia intracellularis infection in a group of pigs in a subclinically affected herd from weaning to slaughter. Vet Microbiol 146: 361-365.
• Dittmar M, Hoelzle LE, Hoelzle K, Sydler T, Corboz L, Miserez R, Wittenbrink MM (2003) Diagnosis of porcine proliferative enteropathy: detection of Lawsonia intracellularis by pathological examinations, polymerase chain reaction and cell culture inoculation. J Vet Med B 50: 332-338.
• Guedes RM, Gebhart CJ, Winkelman NL, Mackie-Nuss RA, Marsteller TA, Deen J (2002) Comparison of different methods for diagnosis of porcine proliferative enteropathy. Can J Vet Res 66: 99-107.
• Hidalgo A, Carvajal A, García-Feliz C, Osorio J, Rubio P (2009) Antimicrobial susceptibility testing of Spanish field isolates of Brachyspira hyodysenteriae. Res Vet Sci 87: 7-12.
• Huerta B, Arenas A, Carrasco L, Maldonado A, Tarradas C, Carbonero A, Perea A (2003) Comparison of diagnostic techniques for porcine proliferative enteropathy (Lawsonia intracellularis infection). J Comp Pathol 129: 179-185.
• Jacobson M, Hård af Segertad C, Gunnarson A, Fellström C, de Verdier Klingenberg K, Wallgren P, Jensen-Waern M (2003) Diarrhoea in the growing pig - a comparison of clinical, morphological and microbial findings between animals from good and poor performance herds. Res Vet Sci 74: 163-169.
• Jacobson M, Fellström C, Jensen-Waern M (2010) Porcine proliferative enteropathy: an important disease with questions remaining to be solved. Vet J 184: 264-268.
• Jensen TK, Boesen HT, Vigre H, Boye M (2010) Detection of Lawsonia intracellularis in formalin-fixed porcine intestinal tissue samples: comparison of immunofluorescence and in-situ hybridization, and evaluation of the effects of controlled autolysis. J Comp Pathol 142: 1-8.
• Jensen TK, Vigre H, Svensmark B, Bille-Hansen V (2006) Distinction between porcine circovirus type 2 enteritis and porcine proliferative enteropathy caused by Lawsonia intracellularis. J Comp Pathol 135: 176-182.
• Ladinig A, Sommerfeld-Stur I, Weissenböck H (2009) Comparative evaluation of diagnostic methods for Lawsonia intracellularis infection in pigs, with emphasis on cases lacking characteristic lesions. J Comp Pathol 140: 140-148.
• Laine TM, Lyytikäinen T, Yliaho M, Anttila M (2008) Risk factors for post-weaning diarrhoea on piglet producing farms in Finland. Acta Vet Scand 50: 21.
• Lawson GH, Gebhart CJ (2000) Proliferative enteropathy. J Comp Pathol 122: 77-100.
• McOrist S, Gebhart CJ, Lawson GH (1994) Polymerase chain reaction for diagnosis of porcine proliferative enteropathy. Vet Microbiol 41: 205-212.
• Opriessnig T, Madson DM, Roof M, Layton SM, Ramamoorthy S, Meng XJ, Halbur PG (2011) Experimental reproduction of porcine circovirus type 2 (PCV2)-associated enteritis in pigs infected with PCV2 alone or concurrently with Lawsonia intracellularis or Salmonella typhimurium. J Comp Pathol 145, 261-270.
• Pedersen KS, Holyoake P, Stege H, Nielsen JP (2010) Diagnostic performance of different fecal Lawsonia intracellularis-specific polymerase chain reaction assays as diagnostic tests for proliferative enteropathy in pigs: a review. J Vet Diagn Invest 22: 487-494.
• Pejsak Z, Żmudzki J, Wasak M, Porowski M, Wojciechowski J, Mirt D (2007) Prevalence of Brachyspira hyodysenteriae and Lawsonia intracellularis infections in Polish swine population. Medycyna Wet 63: 994-996.
• Rohde J, Kessler M, Baums CG, Amtsberg G (2004) Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany. Vet Microbiol 102: 25-32.
• Szczotka A, Zmudzki J, Pejsak Z, Stadejek T (2010) Ocurrence of porcine circovirus type 2 (PCV-2) in cases of antibiotic non-responsive diarrhoea in pigs. J Comp Pathol 143: 326.
• Weissenböck H, Mrakovcic M, Ladinig A, Fragner K (2007) In situ hybridization for Lawsonia intracellularis-specific 16S rRNA sequence in parrafin-embedded tissue using a digoxigenin-labeled oligonucleotide probe. J Vet Diagn Invest 19: 282-285.
• Zlotowski P, Correa AMR, Borba MR, Cruz CEF, Driemeier D (2008) Enteritis associated with Lawsonia intracellularis and porcine circovirus type 2 infection in wild boars in Southern Brazil. Cikncia Rural, Santa Maria 38: 2540-2544.