Isolation and characterization of pyrroline-5-carboxylate synthetase gene from perennial ryegrass (Lolium perenne L.)

• Autorzy: Cao L. , Han L. , Zhang H.-I. , Xin X.-b. , Imtiaz M. , Yi M.-F. , Sun Z.-Y. , Ju G.-S. , Qian Y.-Q. , Liu J.-X.
• Języki publikacji: EN

Abstrakty

EN

Perennial ryegrass is an important turf grass and also used as a forage plant. However, abiotic stresses such as salt and drought are main limitations to its cultivation. In the present work, we cloned the gene encoding pyrroline-5-carboxylate synthetase (P5CS) from Lolium perenne, which is responsible for proline biosynthesis. This gene had a coding sequence (CDS) of 2151 base pairs (bp) encoding 716 amino acids. Multi-alignment analyses showed that the putative Lolium perenne P5CS (LpP5CS) contain all conserved functional sites and regions, and also displayed considerable similarities to Triticum astevum P5CS (TaP5CS) and Oryza sativa P5CSs (OsP5CSs). The real-time polymerase chain reaction (PCR) showed that the LpP5CS was highly expressed in leaves than in other tissues under normal conditions, and induced by sodium chloride (NaCl), abscisic acid (ABA), polyethylene glycol and cold treatments. Furthermore, ectopic expression of LpP5CS led to proline accumulation in tobacco under normal conditions. The transgenic tobacco over-expressing LpP5CS exhibited stronger tolerance to salt and drought as compared to control. These results showed, that LpP5CS responds to stress signals involving salt, drought, cold and ABA in perennial ryegrass. Our data indicate that LpP5CS might be a candidate gene for stressassociated molecular breeding in perennial ryegrass.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 03
• Opis fizyczny: Article: 62 [9 p.], fig.,ref.

Twórcy

autor

Cao L.

• Department of Horticulture and Landscape, Yanbian University, Yanji 13300, China
• State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
• Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100091, China

autor

Han L.

• State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

autor

Zhang H.-I.

• Beijing Institute of Landscape Architecture, Beijing 100102, China
• Beijing Key Laboratory of Greening Plants Breeding, Beijing 100102, China

autor

Xin X.-b.

• Beijing Institute of Landscape Architecture, Beijing 100102, China
• Beijing Key Laboratory of Greening Plants Breeding, Beijing 100102, China

autor

Imtiaz M.

• Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100091, China

autor

Yi M.-F.

• Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100091, China

autor

Sun Z.-Y.

• State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

autor

Ju G.-S.

• State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

autor

Qian Y.-Q.

• State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

autor

Liu J.-X.

• State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

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Identyfikatory

DOI

10.1007/s11738-015-1808-9