Comparison of PCR-based AMOS, Bruce-ladder, and MLVA assays for typing of Brucella species

• Autorzy: Weiner M. , Iwaniak W. , Szulowski K.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was the comparison between three PCR assays: AMOS, Bruce-ladder, and MLVA for typing Brucella reference strains representing different species. The B. abortus bv1, 2, 3, 4, 5, 6, and 9, B. melitensis bv1, 2, and 3, B. suis bv1, 2, 3, 4, and 5, B. ovis, B. canis, and B. neotomae strains were used. It was found that AMOS PCR could correctly identify only B. abortus bv1, 2, and 4, B. suis bv1, three biovars of B. melitensis (bv1, bv2, and bv3), and B. ovis. The remaining reference strains did not generated predicted amplicons or generated unspecific bands. In contrast to AMOS PCR, Bruce-ladder PCR was able to detect DNA from B. canis, B. neotomae, B. abortus bv3, 5, 6, and 9, and B. suis bv2, 3, 4, and 5. MLVA technique correctly recognised the species among B. aborus, B. melitensis, B. ovis, and B. neotomae using VNTR6, VNTR8, VNTR11, VNTR12, VNTR42, VNTR43, VNTR 45, and VNTR55 except the B. abortus bv5, which generated the same VNTR profile as B. abortus bv9. Moreover, MLVA did not differentiate B. canis from B. suis bv4. Only Bruce-ladder correctly identified the species of all tested Brucella strains and could be a useful tool for the rapid identification of species of Brucella strains.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.625-630,fig.,ref.

Twórcy

autor

Weiner M.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Iwaniak W.

autor

Szulowski K.

Bibliografia

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