Comparison of diagnostic methods (wet mount, trichrome staining, formol-ether, PCR, and xenic in vitro culture) for the detection of Blastocystis in stool samples in Urmia educational hospitals, the Northwest of Iran

• Autorzy: Zamani R. , Khademvatan S. , Tappeh K.H. , Diba K. , Abasi E.
• Języki publikacji: EN

Abstrakty

EN

Blastocystis spp. is known as a common intestinal protozoan parasite in human and animals. The parasite has a worldwide distribution and is frequently detected in faecal samples in clinical parasitology laboratories. The goal of the study was to compare the sensitivity and specificity of formol-ether technique (FECT), trichrome staining, xenic in vitro culture (XIVC), microscopy of faecal smears, and polymerase chain reaction (PCR) methods for detecting Blastocystis spp. in human stool samples. The prevalence of the parasite in the stool samples referred to educational hospitals was also determined. A total of 575 cases were assessed to detect the parasite. After collecting from patients referring to Urmia educational hospitals, the samples were examined by microscopy of faecal smears, trichrome staining, FECT, XIVC using Jones’ medium, and PCR, to evaluate the presence of Blastocystis spp. Microscopy of faecal smears, trichrome staining, FECT, and PCR technique detected 94, 100, 96, and 44 positive cases, with the sensitivity of 71.3%, 74.4%, 74.4%, and 80.4% and the specificity of 99.6%, 99.1%, 100%, and 93.1%, respectively. XIVC method identified the highest number of positive cases (129 cases) among the other methods. Our findings indicates that XIVC technique is more sensitive method for the detection of Blastocystis spp. in human stool, as compared to direct smear, trichrome staining, FECT, and PCR methods.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2021
• Tom: 67
• Numer: 4
• Opis fizyczny: p.795-803,fig.,ref.

Twórcy

autor

Zamani R.

• Department of Parasitology and Mycology, Cellular and Molecular Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

autor

Khademvatan S.

• Department of Parasitology and Mycology, Cellular and Molecular Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

autor

Tappeh K.H.

• Department of Parasitology and Mycology, Cellular and Molecular Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

autor

Diba K.

• Department of Parasitology and Mycology, Cellular and Molecular Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

autor

Abasi E.

• Department of Parasitology and Mycology, Cellular and Molecular Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
• Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Identyfikatory

DOI

10.17420/ap6704.398